Sort   by:  
 Page size 
TranscriptomicsPolysome profiling and ribosome footprinting of Drosophila mature oocyte and activated egg
We use mRNA-seq in combination with polysome profiling to determine translational status for all mRNAs in Drosophila mature oocytes and activated eggs. Puromycin-treated lysates are used as a negative control in polysome profiling experiments. Additionally, we use ribosome footprinting to globally m...
ORGANISM(S): Drosophila melanogaster 
2014-06-12 | E-GEOD-52799 | biostudies-arrayexpress
ProteomicsProteomic analysis of polysomes isolated from human cells infected with polio, zika or dengue viruses
Viral pathogens are an ongoing threat to public health worldwide. Dissecting their dependence on host biosynthetic pathways could lead to effective antiviral therapies. To define how entero- and flaviviruses redirect host ribosomes to synthesize viral proteins and disable host protein production, we...
ORGANISM(S): Homo sapiens (Human) 
2021-08-17 | PXD024546 | Pride
Lc-ms/ms Ribosomes Viruses Polysomes Human Infection Protein synthesis Proteostasis Protein folding Translation 
TranscriptomicsMouse cardiac tissue, polysomes and monosomes: Vehicle treated control vs. 25mg/kg DMNQ
Translational profiling of mouse cardiac tissue treated with 25mg/kg DMNQ in 10 ml/kg arachis oil over an acute time course (0.5-120 hours) compared to time matched control animals treated with 10ml/kg saline Two colour microarrays with time matched controls vs 25mg/kg DMNQ cardiac tissue. Before mi...
ORGANISM(S): Mus musculus 
2010-08-06 | E-GEOD-17830 | biostudies-arrayexpress
TranscriptomicsMouse cardiac tissue, polysomes and monosomes: Vehicle treated control vs. 15mg/kg doxorubicin
Translational profiling of mouse cardiac tissue treated with 15mg/kg doxorubicin in 10 ml/kg saline over an acute time course (0.5-120 hours) compared to time matched control animals treated with 10ml/kg saline. Two colour microarrays with time matched controls vs 15mg/kg doxorubicin cardiac tissue....
ORGANISM(S): Mus musculus 
2010-08-05 | E-GEOD-17823 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mRNAs that remained associated with polysomes isolated from human cells after infection with poliovirus
Although most eukaryotic mRNAs need a functional cap binding complex eIF4F for efficient 5' end- dependent scanning to initiate translation, picornaviral, hepatitis C viral, and a few cellular RNAs have been shown to be translated by internal ribosome entry, a mechanism that can operate in the prese...
ORGANISM(S): Homo sapiens 
2005-06-24 | E-SMDB-59 | biostudies-arrayexpress
GenomicsPolysomes after EIF4E6 RNAi
Polysomes after EIF4E6 RNAi
PRJEB52756 | ENA
ProteomicsRibo Mega-SEC Provides a Rapid, Efficient and Reproducible Analysis of Mammalian Polysomes and Ribosomal Subunits
We describe Ribo Mega-SEC, a powerful approach for the separation and biochemical analysis of mammalian polysomes and ribosomal subunits using Size Exclusion Chromatography and uHPLC, which was achieved within 15 min from sample injection to fraction collection. Ribo Mega-SEC reproducibly shows tran...
ORGANISM(S): Mus musculus (Mouse) 
2018-08-10 | PXD008913 | Pride
Ribosome 60s 40s Polysome Liver Sec 
TranscriptomicsPolysomes
Translational profiles of wild type yeast strains were identified by microarray analysis by isolating polysomes using sucrose gradients to recover polysome-associated RNA and comparing polysome-associated RNA vs total RNA
ORGANISM(S): Saccharomyces cerevisiae 
2013-04-05 | GSE45797 | GEO
TranscriptomicsFunctional characterization of C. elegans Y-box binding proteins reveals tissue-specific functions and a critical role in the formation of polysomes (ribosome)
We present a basic characterization of the function of Y-box binding proteins in C. elegans. Besides playing an important role for fertility in the germline (all four CEY proteins), we found that the presence of CEY-1 and CEY-4 is essential for the assembly of larger polysomes in the soma. We theref...
ORGANISM(S): Caenorhabditis elegans 
2015-01-08 | E-GEOD-62859 | biostudies-arrayexpress
GenomicsRNA-seq of Trypanosoma brucei polysomes in response to ZC3H5 RNAi knockdown
RNA-seq of Trypanosoma brucei polysomes in response to ZC3H5 RNAi knockdown
PRJEB33092 | ENA
Sort   by:  
 Page size