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ProteomicsPost-column solvent addition enhances sensitivity of microflow-based bottom-up proteomics
We demonstrate that post-column addition of organic solvents leads to a strong enhancement of electrospray stability in microflow LC-MS experiments improving the sensitivity across the entire gradient, for early eluting peptides by up to tenfold. Post-column solvent addition did not negatively affec...
ORGANISM(S): Homo sapiens (Human) 
2019-05-28 | PXD011970 | Pride
Post-column solvent addition Hela digest Microflow lc-ms Bottom-up proteomics 
TranscriptomicsIn vitro antiplasmodial activity of Dicoma anomala subsp. gerrardii (Asteraceae): identification of its main active constituent and gene expression profiling.
To determine the transcriptional effects of a novel plant-based compound, dehydrobrachylaenolide, on P. falciparum, parasite cultures were treated with the compound over time. Samples were taken for analysis 2, 6, and 12 hours post-invasion of human red blood cells. Control cultures were treated sim...
ORGANISM(S): Plasmodium falciparum 3D7 
2011-11-01 | E-GEOD-29874 | biostudies-arrayexpress
MetabolomicsMetabolic Profiling of Escherichia coli-Based Cell-Free Expression Systems for Process Optimization
Biotechnology has transformed the production of various chemicals and pharmaceuticals due to its efficient and selective processes, but it is inherently limited by its use of live cells as 'biocatalysts.' Cell-free expression (CFE) systems, which use a protein lysate isolated from whole cells, have ...
2020-06-02 | MTBLS1079 | MetaboLights
UnknownThe comparison of CHCA solvent compositions for improving LC-MALDI performance and its application to study the impact of aflatoxin B1 on the liver proteome of diabetes mellitus type 1 mice.
Not available
S-EPMC5524319 | biostudies-literature
ProteomicsProteome characterization of HCMV virus microenvironment with addition of cidofovir
The control of host processes which influence cell-cell communication is central to determining the outcome of a virus infection in tissue. Despite this, it remains unclear how cells either in close or distant proximity to an infected cell differentially respond to the primary infection. Here, we es...
ORGANISM(S): Homo sapiens (Human) 
2023-05-11 | PXD040582 | Pride
Hcmv Innate immunity Membrane contact sites Mass spectrometry Virus microenvironment Cell cycle Cidofovir 
MetabolomicsMitochondrial metabolism sustains DNMT3A-R882-mutant clonal haematopoiesis
Somatic DNMT3A R882 codon mutations drive the most common form of clonal haematopoiesis (CH) and are associated with increased acute myeloid leukaemia (AML) risk1,2. Preventing expansion of DNMT3A-R882-mutant haematopoietic stem/progenitor cells (HSPCs) may therefore avert progression to AML. To ide...
2025-02-06 | MTBLS12201 | MetaboLights
TranscriptomicsMechanistic analysis of the model obesogen Tributyltin in the 3T3-L1 cell line
Obesogenic compounds are chemicals that might have an influence on obesity development through in utero or chronic lifetime exposure. Tributyltin (TBT) is one of the most studied obesogenic compounds, inducing adipogenesis in vitro and increasing body fat after in utero exposure of mice. This study ...
ORGANISM(S): Mus musculus 
2013-02-25 | E-GEOD-41340 | biostudies-arrayexpress
UnknownCharacterization of therapeutic proteins by cation exchange chromatography-mass spectrometry and top-down analysis.
Not available
S-EPMC7188404 | biostudies-literature
TranscriptomicsTranscriptional response of Escherichia coli O157 TUV93-0 to small molecule inhibitors of TTS
The transcriptional profile of Escherichia coli O157 treated with small molecule inhibitors of type III secretion was determined. Four variations of the small molecule inhibitor were assessed for global changes in transcription by treating cells with 20uM of inhibitor or an equivalent volume of DMSO...
ORGANISM(S): Escherichia coli 
2009-08-10 | E-GEOD-10319 | biostudies-arrayexpress
ProteomicsCounterion Optimization Dramatically Improves Selectivity for Phosphopeptides and Glycopeptides in Electrostatic Repulsion-Hydrophilic Interaction Chromatography (ERLIC)
A well-hydrated counterion can selectively and dramatically increase retention of a charged analyte in hydrophilic interaction chromatography (HILIC). The effect is enhanced if the column is charged, as in electrostatic repulsion-hydrophilic interaction chromatography (ERLIC). This combination was...
ORGANISM(S): Mus musculus (Mouse) 
2021-06-01 | PXD022988 | Pride
Peptide Fraction Ptm Glyco Phosphate Erlic 
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