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UnknownShotgun Proteomics of Human Dentin with Different Prefractionation Methods.
Not available
S-EPMC6418255 | biostudies-literature
ProteomicsSpin column-based prefractionation alternatives for Streamlined Tandem Mass Tag (SL-TMT) sample processing
Fractionation is essential to achieving deep proteome coverage for sample multiplexing experiments where currently up to 18 samples can be analyzed concurrently. However, prefractionation (i.e., upstream of LC-MS/MS analysis) with a liquid chromatography system constrains sample processing as only a...
ORGANISM(S): Homo sapiens (Human) 
2023-12-28 | PXD038383 | Pride
Faims Tmtpro Hprp Isobaric tagging Sax 
ProteomicsProteome-wide evaluation of plasma depletion methods vis-à-vis fractionation techniques
Human plasma is one of the most widely used tissues in clinical analysis, and plasma-based biomarkers are used for monitoring patient health status and/or response to medical treatment to avoid unnecessary invasive biopsy. Data driven plasma proteomics has suffered from a lack of throughput and dete...
ORGANISM(S): Homo sapiens (Human) 
2021-02-17 | PXD022469 | Pride
Electrophoresis Plasma Chromatography Mass spectrometry Prefractionation techniques Label-free quantitation Biomarkers Proteome 
ProteomicsPEPPI-MS- and GELFrEE-fractionated E. coli MG1655 whole cell lysate
Supplemental Files accompanying manuscript “PEPPI-MS: polyacrylamide gel-based prefractionation for analysis of intact proteoforms and protein complexes by mass spectrometry”.
ORGANISM(S): Escherichia Coli Str. K-12 Substr. Mg1655 (ncbitaxon:511145) 
2020-04-08 | MSV000085258 | MassIVE
ProteomicsK562 cell lysates prefractionated by various methods
K562 cell lysates were prefractionated by protein reversed-phase chromatography (Prot-RP), SDS-PAGE, and high-pH peptide RP (Pept-RP) to compare their method characteristics. The starting material was prefractionated to 10 fractions for each method. Each prefractionation method was conducted in trip...
ORGANISM(S): Homo Sapiens (human) 
2024-10-15 | PXD049312 | JPOST Repository
ProteomicsUnveiling the unfolded proteome: evaluation and comparison of methods for the enrichment of intrinsically disordered proteins
Intrinsically disordered proteins (IDPs) comprise a significant fraction of the eukaryotic proteome and are characterised by the absence of stable secondary or tertiary structures under physiological conditions. Despite their functional importance, comprehensive proteomic analysis of IDPs remains a ...
ORGANISM(S): Homo sapiens (Human) 
2026-02-13 | PXD070700 | Pride
Heat extraction Lc-ms Intrinsically disordered proteins Prefractionation methods Label-free quantification Perchloric acid extraction Disorder prediction algorithm Instrinsically disordered proteins enrichment 
ProteomicsPEPPI-SP3: Robust and Simple Sample Prefractionation Workflow for Top-Down Proteomics
In this study, we developed a complete, robust, and simple sample preparation workflow named PEPPI-SP3 for top-down proteomics by combining PEPPI-MS with the magnetic bead-based protein purification approach used in SP3 (single-pot, solid-phase-enhanced sample preparation), now one of the standard s...
ORGANISM(S): Homo Sapiens (human) 
2025-01-23 | PXD056413 | JPOST Repository
ProteomicsComplementary metaproteomic approaches to assess marine bacteriopankton response towards algal bloom
In this project, the metaproteome of the marine bacterioplankton was analyzed to assess its respone towards an algal bloom in the southern North Sea in spring 2010. Proteins were extracted applying two different methods: (i) applying chemical cell lysis using trifluoroethanol in combination with in-...
ORGANISM(S): environmental samples 
2017-04-11 | PXD004944 | Pride
Metaproteome Algal bloom Marine bacterioplankton 
ProteomicsProteome-wide evaluation of plasma depletion methods vis-à-vis fractionation techniques
Human plasma is one of the most widely used tissues in clinical analysis, and plasma-based biomarkers are used for monitoring patient health status and/or response to medical treatment to avoid unnecessary invasive biopsy. Data driven plasma proteomics has suffered from a lack of throughput and dete...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-06-25 | MSV000098333 | MassIVE
UnknownComparison of strong cation exchange and SDS-PAGE fractionation for analysis of multiprotein complexes.
Not available
S-EPMC3707127 | biostudies-literature
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