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ProteomicsHydrophobic tagging-assisted N-termini enrichment (HYTANE) for in-depth N-terminome analysis
The analysis of protein N-termini is of great importance for understanding the protein function and elucidating the proteolytic processing. Herein, we develop a negative enrichment strategy, termed as hydrophobic tagging-assisted N-termini enrichment (HYTANE) to achieve a global N-terminome analysis...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2016-08-19 | PXD004690 | Pride
Sacchoromyces cerevisiae Jurkat N-termini Caspase 
GenomicsHigh-throughput pyrosequencing of forum domains termini (FT) isolated and amplified from human HEK293T cells
Forum domains are stretches of chromosomal DNA that are excised from eukaryotic chromosomes during their spontaneous non-random fragmentation. Mostly forum domains are of 50-200 kb in length, although larger domains, up to 500 - 700 kb, are also observed. We performed a genome-wide mapping of forum ...
ORGANISM(S): Homo sapiens 
2013-03-31 | E-GEOD-35065 | biostudies-arrayexpress
ProteomicsHUNTER N-termini profiling of Arabidopsis thaliana wt and vpe0 mutants
Comparison of protein termini in Arabidopsis thaliana vpe0 quadruple mutant and wildtype seedlings shortly after germination to identify differential processed proteins.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-09-03 | PXD012804 | Pride
Arabidopsis vpe0 termini degradomics hunter 
ProteomicsHUNTER N-termini profiling of Arabidopsis thaliana leaves using different proteases
Comparison of protein N-termini in Arabidopsis thaliana leaves by the HUNTER method; the corresponding digestion was performed with legumain, GluC and trypsin.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-01-20 | PXD014697 | Pride
Arabidopsis hunter n-termini profiling 
ProteomicsC-terminomics E. coli - C-terminal charge-reversal derivatization and parallel use of multiple proteases facilitates identification of protein C-termini by C-terminomics
The identification of protein C-termini in complex proteomes is challenging due to the poor ionization efficiency of the carboxyl group. Amidating the negatively charged C-termini with ethanolamine (EA) has been suggested to improve detection of C-terminal peptides and allows for a directed depletio...
ORGANISM(S): Escherichia coli 
2016-03-11 | PXD003342 | Pride
Cid Hcd Etd C-terminus Derivatization 
ProteomicsProfiling of Native and Protease_Generated Protein Amino Termini from Formalin-Fixed, Paraffin-Embedded Tissues
Dysregulated proteolysis represents a hallmark of numerous diseases. In recent years, increasing number of studies has begun looking at the protein termini in hope to unveil the physiological and pathological functions of proteases in clinical research. However, the availability of cryopreserved tis...
ORGANISM(S): Mus musculus (Mouse) 
2016-04-25 | PXD002847 | Pride
Ffpe Protease Degradome Tails Proteolysis N-terminomics 
ProteomicsN-terminome profiling in HEK293T
Various forms of the protein, called proteoform, are generated by co- or post-translational modification, alternative splicing and alternative translation initiation site, resulting in interactions between ribosomes, mRNAs, tRNAs, and various enzymes. Here, we introduce a N-terminal peptide enrichme...
ORGANISM(S): Homo sapiens (Human) 
2017-08-17 | PXD005583 | Pride
Protein stability Alternative translational initiation site Acetylation N-terminome Positional proteomics Proteoform N-termini Fasp N-end rule Translational initiation site 
ProteomicsA protein microarray-based analysis of S-nitrosylation
Yeast protein microarrays were utilized to investigate determinants of S-nitrosylation by biologically relevant low-mass S-nitrosothiols (SNOs). Large numbers of S-nitrosylated yeast proteins were identified after treatment with SNOs, among which those with active-site Cys thiols residing at N termi...
ORGANISM(S): Saccharomyces cerevisiae 
2010-05-19 | E-GEOD-19218 | biostudies-arrayexpress
TranscriptomicsCapture and sequence analysis of RNAs with 5'-hydroxyl termini in S. cerevisiae
Data from 5OH-seq experiments to map RNAs with 5'-hydroxyl termini in S. cerevisiae 17 samples of yeast RNA analyzed by 5OH-seq. 2 Replicates: +/- Xrn1,+/- Tm, +/- SAP. 1 replicate: pre-fragmented RNA
ORGANISM(S): Saccharomyces cerevisiae 
2015-04-18 | E-GEOD-61527 | biostudies-arrayexpress
ProteomicsAnalysis of mitochondrial N termini by HUNTER (High-efficiency Undecanal-based N Termini EnRichment)
Protein N-termini reveal fundamental regulatory mechanisms and their perturbation in disease. We present a robust, sensitive, scalable and automatable method called High-efficiency Undecanal-based N Termini EnRichment (HUNTER) for system-wide identification of thousands of N-termini from minute samp...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-02-28 | MSV000083504 | MassIVE
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