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ProteomicsC-terminomics E. coli - C-terminal charge-reversal derivatization and parallel use of multiple proteases facilitates identification of protein C-termini by C-terminomics
The identification of protein C-termini in complex proteomes is challenging due to the poor ionization efficiency of the carboxyl group. Amidating the negatively charged C-termini with ethanolamine (EA) has been suggested to improve detection of C-terminal peptides and allows for a directed depletio...
ORGANISM(S): Escherichia coli 
2016-03-11 | PXD003342 | Pride
Cid Hcd Etd C-terminus Derivatization 
MetabolomicsAdvancing Reef Monitoring Techniques through Exometabolomics: Quantification of Labile Dissolved Organic Metabolites on Coral Reefs

Extracellular chemical cues constitute much of the language of life among marine organisms from microbes to mammals. These chemical cues shape foraging and feeding strategies, symbiotic interactions, selection of mates and habitats, and the transfer of energy and nutrient...

2025-07-16 | MTBLS9008 | MetaboLights
ProteomicsMicrowave oven-assisted derivatization of histone proteoforms using trimethylacetic anhydride
We introduce a new reagent – Trimethylacetic anhydride (TMA) for highly efficient histone derivatization, which is facilitated by microwave irradiation to reduce sample preparation duration. The derivatization of unmodified NH2- groups at histone lysine residues is necessary prior LC-MS analysis usi...
ORGANISM(S): Homo sapiens (Human) 
2021-06-14 | PXD019502 | Pride
O histone derivatization Epigenetics Mass spectrometry Trimethylacetic anhydride 
GenomicsFunctional evolution, modification, and derivatization of mammalian developmental enhancers
Functional evolution, modification, and derivatization of mammalian developmental enhancers
PRJNA1454538 | ENA
ProteomicsA Derivatization-Free PRM Workflow for Quantitative, Site-Specific Analysis of Histone Post-Translational Modifications
Histone post-translational modifications (PTMs) are key regulators of chromatin architecture and gene expression. Although DIA-based platforms for histone PTM quantification are available, multiplexed targeted assays remain underdeveloped. In addition, bottom-up workflows frequently rely on chemical...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2026-09-09 | PXD071393 | Pride
Ptms Prm Skyline Quantitation Histone Argc 
ProteomicsReversible Lysine Derivatization Enables High Specificity of Arg-C Digestion Using Trypsin
Bottom-up proteomics approach has become an important strategy in diverse areas of biological research, and the enzymatic digestion is essential for this technology. Endopeptidase Arg-C catalyzing the hydrolytic cleavage of peptide bonds C-terminal to arginine could be an important protease in botto...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2018-01-04 | PXD007994 | Pride
Proteomics Arg-c Trypsin Lys-c Citraconylation Lc-ms/ms 
ProteomicsCleavable Hydrophobic Derivatization Strategy for Enrichment and Identification of Phosphorylated Lysine Peptides
Herein, a cleavable hydrophobic derivatization (CHD) strategy was established for the enrichment and identification of pLys peptides. By CHD, 2,5-dioxopyrrolidin-1-yl-3-(decyldisulfanyl)propanoate (Dec-disulf-NHS) was synthesized to react with lysine dephosphorylated peptides, and then the derived p...
ORGANISM(S): Escherichia Coli 
2019-02-12 | PXD012682 |
ProteomicsN-Terminal Protein Sequencing by Nicotinic Acid Derivatization and MS Analysis
Most MS-based workflows for N-terminal protein sequence analysis rely on the modification of primary amines at the protein N-terminus and lysine residues, in many cases through dimethylation. However, incomplete dimethyl labeling and undesired side reactions have been reported previously and were al...
ORGANISM(S): Bos taurus (Bovine) Escherichia coli Equus caballus (Horse) 
2026-08-17 | PXD081064 | Pride
Protein sequencing Dimethylation N-terminomics Nicotinic acid N-terminal 
ProteomicsArg-C Ultra simplifies histone preparation for LC-MS/MS
Using mammalian histone extract, we demonstrate that Arg-C Ultra facilitates histone preparation for LC-MS/MS. We show the performance of Arg-C Ultra in terms of digestion specificity, number of modified forms identified, and yield of quantitative information compared with standard Arg-C and trypsin...
ORGANISM(S): Homo sapiens (Human) 
2025-06-30 | PXD059514 | Pride
Histone Arg-c ultra Trimethylacetic anhydride Lc-ms/ms Chemical derivatization 
OtherFunctional evolution, modification, and derivatization of mammalian developmental enhancers
This dataset encompasses two complementary massively parallel reporter assay (MPRA) studies that together dissect the functional architecture and evolutionary dynamics of mammalian cis-regulatory elements (CREs) at nucleotide resolution, using five parietal endoderm enhancers as a model system. The ...
ORGANISM(S): Mus musculus 
2026-04-20 | GSE328309 | GEO
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