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ProteomicsOne-Pot Time-Induced Proteome Integral Solubility Alteration (OPTI-PISA) assay for automated and sensitive drug target identification
Proteome Integral Solubility Alteration (PISA) assay is widely used for identifying drug targets, but it is labour-, sample and time-consuming. Aiming at enabling automation and greatly reducing the sample amount, we developed One-Pot Time-Induced (OPTI)-PISA. Here we demonstrate OPTI-PISA performan...
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD050241 | Pride
Drug target Proteome integral solubility alteration (pisa) One pot Automation 
ProteomicsSelection of Heating Temperatures Improves the Sensitivity of Proteome Integral Solubility Alteration (PISA) Assay
The thermal shift assay is a robust method of discovering protein-ligand interactions by measuring the alterations in protein thermal stability under various conditions. Several thermal shift assays have been developed and their throughput has been advanced greatly by the rapid progress in tandem ma...
ORGANISM(S): Homo sapiens (Human) 
2020-04-06 | PXD017346 | Pride
Proteome integral solubility alteration (pisa) assay Human cell line lysate Lc-ms/ms 
ProteomicsExploring the Anti-Cancer mechanism of Cardiac Glycosides using proteome integral solubility alteration approach
Cancer remains a significant global health challenge, necessitating the development of innovative and cost-effective treatments. Drug repurposing, particularly of cardiac glycosides (CGs), offers a promising strategy to expedite the discovery of anti-tumor drugs. This study employs proteome integral...
ORGANISM(S): Homo Sapiens 
2024-05-31 | PXD052740 |
ProteomicsStreamlined analysis of drug targets by proteome integral solubility alteration indicates organ-specific engagement
Proteins are the primary targets of almost all small molecule drugs. However, even the most selectively designed drugs can potentially target several unknown proteins. Identification of potential drug targets can facilitate design of new drugs and repurposing of existing ones. Current state-of-the-a...
ORGANISM(S): Homo sapiens (Human) Rattus norvegicus (Rat) 
2024-10-16 | PXD050784 | Pride
Drug target Small molecule Target identification Chemical proteomics Tpp Pisa 
ProteomicsSystem-wide profiling of drug residence time by proteome integral solubility alteration assay
Most drugs used in the clinic and drug candidates target multiple proteins, and thus detailed characterization of their efficacy targets is required. While current methods rely on quantitative measurements at thermodynamic equilibrium, kinetic parameters such as the residence time of a drug on its t...
ORGANISM(S): Homo sapiens (Human) 
2023-01-06 | PXD034351 | Pride
Target engagement Drug development Thermal proteome profiling. Efficacy target Lead compound optimization Target prioritization Binding affinity Precision medicine Target occupancy 
ProteomicsProteome integral solubility alteration assay combined with multi-criteria decision-making analysis for developing adverse outcome pathways
To simplify chemical risk assessment, the adverse outcome pathway (AOP) method has arisen as a framework to predict the impact of chemical exposure on human and environmental health. The development of this predictive tool requires knowledge of the molecular interaction between chemicals and protein...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD033056 | Pride
Hepatocytes Bioactive compound Pollutants Proteome integral solubility alteration assay Tcdd Analytical hierarchy process Hepg2 
ProteomicsProteome integral solubility alteration (PISA) analysis of compound treated Haemonchus contortus
This study applied the proteome integral solubility alteration (PISA) assay to infer molecular targets and protein–ligand interactions of candidate nematocidal compound in Haemonchus contortus. By monitoring compound-induced shifts in protein solubility across a temperature gradient, the analysis pr...
ORGANISM(S): Haemonchus contortus (Barber pole worm) 
2026-07-19 | PXD075263 | Pride
Haemonchus contortus Proteomics Anthelmintic Pisa assay 
ProteomicsProteome integral solubility alteration assay for monitoring antibody-antigen interactions in cells
Proteome Integral Solubility Alteration (PISA) assay is normally used for characterization of the interactions between a small molecule and the cellular proteome. Here we modified PISA uniquely to identify the antigen (tumor necrosis factor alpha) in activated monocytes using the monoclonal antibody...
ORGANISM(S): Homo sapiens (Human) 
2026-05-20 | PXD065457 | Pride
Pisa Antibody Antigen 
ProteomicsTargets of histone deacetylase inhibitors in ovarian cancer identified using proteome integral solubility alteration
Using proteome integral solubility alteration (PISA) to assess HDAC inhibitor targets across ovarian cancer and acute lymphoblastic leukemia cell types
ORGANISM(S): Homo sapiens (Human) 
2024-12-19 | PXD055375 | Pride
Cancer Pisa Hdac inhibitor Hdac Hgsoc Thermal profiling All 
ProteomicsThermoTargetMiner: Proteome Integral Solubility Alteration (PISA) target database for prospective lung cancer drug candidates
Knowledge of the targets of therapeutic compounds helps understanding the compound action mechanism as well as the side-effects. The multiple complementary techniques needed for comprehensive target characterization must combine data reliability with sufficient analysis throughput. Here, we test the...
ORGANISM(S): Homo sapiens (Human) 
2026-06-08 | PXD054158 | Pride
Human Proteomics Lc-ms Lung cancer 
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