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Unknown⿿Comparing the proteome of snap frozen, RNAlater preserved, and formalin-fixed paraffin-embedded human tissue samples.
Not available
S-EPMC5988570 | biostudies-literature
ProteomicsQuantative proteomic analysis of teh central amygdala in neuropathic pain model rats
Neuropathic pain is a major clinic probelm as it is very difficult to treat and mechanism remain unknown. Here, we investigated the differential expression of proteins in the central nuecleus of amygdala (CeA) in neuropathic pain moldel spinal nerve transection (SNT)in rats. CeA was excised from nai...
ORGANISM(S): Rattus norvegicus (Rat) 
2020-02-21 | PXD017473 | Pride
2d-gel Spinal nerve transcection Ncentral amygdala Lc-ms/ms 
Proteomicshuman lung adenocarcinoma cell lines TMT based proteomics
In our study, we found that profilin 1 (PFN1) promoted non-small cell lung cancer (NSCLC) metastasis.For further investigate the mechanisms of PFN1's roles in NSCLC metastasis, we constructed PFN1 overxpression H1299 cell lines(H1299 PFN1 OE cells). And we sent samples of H1299 PFN1 OE cells and EV ...
ORGANISM(S): Homo sapiens (Human) 
2022-04-14 | PXD033148 | Pride
Lung adenocarcinoma cell line Tmt Human 
ProteomicsHuman colon biopsies (healthy and Ulcerative Colitis) LC-MS/MS
The etiology of the inflammatory bowel diseases, including ulcerative colitis, remains incomplete, but recent findings points to the involvement of complex host-microbial interactions. We hypothesized that an analysis of the proteins on the host-microbial interacting surface, the intestinal mucosa, ...
ORGANISM(S): Homo sapiens (Human) 
2015-05-26 | PXD001608 | Pride
Human Control Biopsies Lc-ms Q exactive Ulcerative Colon Healthy Colitis 
ProteomicsMechanism research of Lian Qiao Wen Dan Decoction blocking gastric carcinogenesis
Label-free quantative proteomic techniques to evaluate the effect of Lian Qiao Wen Dan Decoction blocking gastric carcinogenesis
ORGANISM(S): Rattus Norvegicus 
PXD053549 |
ProteomicsUterine fluid extracellular vesciles proteome in bovine clinical and subclinical endometritis
Uterine fluid (UF) samples were acquired from healthy cows, cows with clinical (CLE) and subclinical endometritis (SE). The extracellular vesicles (EVs) were isolated using size exclusion chromatography-based method. The enrichment of UF-EVs were confirmed with mass-spectrometry of a sample before a...
ORGANISM(S): Bos taurus (Bovine) 
2026-04-27 | PXD044219 | Pride
Uterine fluid Extracellular vesicles Endometritis Bovine Lc/ms-ms 
ProteomicsProteome quantification in WASP wild-type and deficient macrophages using DIA-MS
We knocked out Wiskott-Aldrich Syndrome protein (WASP) in an iPSC line and derived the cells to differentiate into macrophages. We found deficiency of WASP results in overexpression of splicing factors and irregulaly sized nulcear speckles. We performed DIA-MS based quantative proteome analysis for...
ORGANISM(S): Homo sapiens (Human) 
2022-04-21 | PXD033326 | Pride
Human Macrophages Dia-ms 
ProteomicsHuman colon biopsies (healthy and Ulcerative Colitis) LC-MS/MS
The etiology of the inflammatory bowel diseases, including ulcerative colitis, remains incomplete, but recent findings points to the involvement of complex host-microbial interactions. We hypothesized that an analysis of the proteins on the host-microbial interacting surface, the intestinal mucosa, ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-31 | MSV000080833 | MassIVE
ProteomicsQuantative pTyr protein complex
Our current research focuses on the complex-centric quantification of pTyr protein complexes. We initially constructed a tri-functional probe based on pTyr superbinder (SH2-S) for the enrichment of pTyr protein complexes. The pTyr protein complexes were separated by ion exchange chromatography (IEC)...
ORGANISM(S): Homo Sapiens 
PXD052742 |
ProteomicsQuantitative proteomic analysis of breast cancer tissues
We compared label-free and SILAC based quantative methods in combination with shotgun, directed and targeted MS methods in laser capture microdissected breast cancer tissues (biological replicates) as well as whole tissue lysates (technical replicates). We aim to recommend a quantitative method for ...
ORGANISM(S): Homo sapiens (Human) 
2013-08-23 | PXD000278 | Pride
Human breast cancer Nanolc-orbitrap-ms Laser capture microdissection Quantitative proteomics 
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