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MetabolomicsQuantitative LC-MS study of compounds found predictive of COVID-19 severity and outcome

INTRODUCTION: Since the beginning of the SARS-CoV-2 pandemic in December 2019 multiple metabolomics studies have proposed predictive biomarkers of infection severity and outcome. Whilst some trends have emerged, the findings remain intangible and uninformative when it comes to n...

2024-04-23 | MTBLS7429 | MetaboLights
ProteomicsHuman, melanoma, p62/SQSTM1, quantitative proteomics
p62/SQSTM1 was identified as a modulator of metastatic genes selectively enriched in melanoma in autophagy independent manner. iTRAQ quantitative proteomic approach was performed in melanoma cell lines (SK-Mel-103 and UACC-62) deficient for p62 to identify downstream effectors of p62. Similar studie...
ORGANISM(S): Homo sapiens (Human) 
2022-02-28 | PXD006650 | Pride
Human Ap-ms P62/sqstm1 Melanoma cell lines Quantitative proteomics Lc-ms/ms 
UnknownAccurate protein complex retrieval by affinity enrichment mass spectrometry (AE-MS) rather than affinity purification mass spectrometry (AP-MS).
Not available
S-EPMC4288248 | biostudies-literature
ProteomicsBait correlation improves interactor identification by TMT-AP-MS
The quantitative multiplexing capacity of isobaric Tandem Mass Tags (TMT) has increased the throughput of affinity purification mass spectrometry (AP-MS) to characterize protein interaction networks of immunoprecipitated baits. However, variable bait levels between replicates can convolute interacto...
ORGANISM(S): Homo sapiens (Human) 
2020-06-22 | PXD016613 | Pride
Tmt Protein-protein interactions Ap-ms 
ProteomicsRat Brain LC-MS/MS - Quantitative Proteomics Reveals Protein-Protein Interactions with Fibroblast Growth Factor12 as a Component of the Nav1
Voltage-gated sodium channels are responsible for the initiation and propagation of action potentials in excitable cells. While the channel is functional on its own, it is the transient and stable protein-protein interactions that modulate functional outcomes. AP-MS has been successfully applied to ...
ORGANISM(S): Rattus norvegicus (Rat) 
2015-03-03 | PXD000719 | Pride
Ap-ms Mammalian brain Interactome 
ProteomicsAnalalysis of ASPP2 interaction
Quantitative AP-MS from HEK 293T cells expressing Strep-SHA tagged ASPP2 or ASPP2. (Ex1) Quantitative AP-MS from HEK293 cells expressing Strep-SHA tagged PP1alpha, beta, gamma. (Ex2)
ORGANISM(S): Homo sapiens (Human) 
2019-04-02 | PXD012378 | Pride
Lc-msms 
ProteomicsAP-MS of helicase Upf1 in PC12 cells
DNA/RNA helicase Upf1 seems to interact with axonal transcripts in response to nerve growth factor (NGF, A. Ludanyi, M. Gaspari and A. Riccio, unpublished data). In order to shed light on Upf1 mechanism of action and to identify potential associations with molecules having cleavage activity, we perf...
ORGANISM(S): Rattus norvegicus (Rat) 
2021-09-10 | PXD023586 | Pride
Protein complex Hud Ago2 Impa1 
ProteomicsQuantitative fragmentomics to map affinities of interactomes
~500,000 protein interactions have been identified in human but most binding affinities remain unknown, limiting quantitative investigation of interactome-function relationships. This gap can be filled by systematically measuring the affinities of minimal interacting protein fragments, the building ...
ORGANISM(S): Homo sapiens (Human) 
2022-10-14 | PXD027743 | Pride
Complexome Pdz Domain-motif interaction Interactome 
ProteomicsHighly accurate protein complex retrieval by affinity enrichment MS rather than affinity purification MS
Protein-protein interactions are fundamental to the understanding of biological processes. Affinity purification coupled to mass spectrometry (AP-MS) is one of the most promising methods for their investigation. Previously, complexes were purified as much as possible, frequently followed by identifi...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2015-03-26 | PXD000955 | Pride
Ap-ms Ae-ms Yeast Interaction proteomics Label-free quantification 
ProteomicsIdentification of cell cycle dependent interaction partners of the septins by quantitative mass spectrometry
The septins are a conserved family of GTP-binding proteins that, in the baker's yeast, assemble into a highly ordered array of filaments at the mother bud neck. These filaments undergo dramatic structural rearrangements during the cell cycle which are mechanistically not yet understood. We aimed at ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2016-01-28 | PXD002561 | Pride
Septins Yeast Silac Cell cycle Quantitative proteomics Ap-ms Spliff 
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