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ProteomicsThe value of subcellular fractionation for protein identification in a B-cell line (Ramos)
Integrated in the Chromosomic-centric Human Proteome Project (C-HPP), a lymphocytic B-cell line (Ramos) was selected as a model of B-cells. We performed a subfractionation strategy (in triplicate) for the extraction of proteins from 4 different fractions (cytoplasm, CYT; organella, ORG; membrane, MB...
ORGANISM(S): Homo sapiens (Human) 
2015-08-04 | PXD001933 | Pride
Cytoplasm B-cell Human Organella Nucleus Orbitrap Ramos Membrane 
ProteomicsLong term adaptation of lymphoma cells to hypoxia
This study explores the long-term adaptation mechanisms of lymphoma cells subjected to hypoxic conditions, with an emphasis on the HBL2 and Ramos cell lines under normoxia and 1% O2 environments. The research methodology encompassed lysing cells using a buffer containing sodium deoxycholate and TEAB...
ORGANISM(S): Homo sapiens (Human) 
2025-02-27 | PXD050227 | Pride
Lymphoma cells (hbl2 Tmt proteomic analysis Hypoxia adaptation Ramos) Cellular mechanisms 
ProteomicsSubcellular fractionation in Ramos B-cells
In order to increase the proteome coverage, a subfractionation strategy has been performed to obtain proteins belonging to four different cellular compartments (i.e., cytoplasm (CYT), membrane (MB), organelle (ORG), and nucleus (NUC)). Experiments have been done in triplicate.
ORGANISM(S): Homo sapiens (Human) 
2022-02-14 | PXD003939 | Pride
Subfractionation B-cells Human Ramos Lc-ms/ms 
ProteomicsColumbia River sediment HUM-V (Hyporheic Uncultured Microbial and Viral) database metaproteome
Metaproteomic data for Rodriguez-Ramos, et al. interrogating microbial and viral communities of hyporheic river sediments within the Columbia River. Samples were digested with trypsin, and analyzed by LC-MS/MS. Data was searched with MS-GF+ using PNNL's DMS Processing pipeline.
ORGANISM(S): Environmental Samples <bacteria> (ncbitaxon:48479) 
2021-04-28 | MSV000087330 | MassIVE
ProteomicsProteome alteration in Myeloma, MGUS to MM transition
We have measured the proteomic changes on ANBL6, IH1, INA6, U266, KJON, OH2, VOLIN, LR5, KARPAS, RAMOS cell lines and used it to build a super SILAC library in order to quantify proteomic changes from MGUG to MM stages of Myeloma with Elite orbitrap mass spetrometer.
ORGANISM(S): Homo sapiens (Human) 
2024-06-07 | PXD006458 | Pride
Lr5 Oribitrap Volin Anbl6 Myeloma Oh2 U266 Silac Kjon Karpas Ih1 Super Ina6 Ramos 
ProteomicsEffect of hypoxia and treatment with cyclosporine A on chemotaxis of RAMOS B cells
Background: Hypoxia is a potent molecular signal for cellular metabolism, mitochondrial function, and migration. Conditions of low oxygen tension trigger regulatory cascades mediated via the highly conserved HIF-1 α post-translational modification system. In the adaptive immune response, B cells (Bc...
ORGANISM(S): Homo sapiens (Human) 
2023-07-20 | PXD037004 | Pride
Cyclosporine Hypoxia Hif1a Immunosuppressant B cells Cxcl12 
UnknownMutations in each of the tol genes of Pseudomonas putida reveal that they are critical for maintenance of outer membrane stability.
Not available
S-EPMC111352 | biostudies-literature
UnknownMultistep IgE Mast Cell Desensitization Is a Dose- and Time-Dependent Process Partially Regulated by SHIP-1.
Not available
S-EPMC9986054 | biostudies-literature
ProteomicsIdentification of spidroin proteins in the silk glands of Nephila clavipes spiders
Glands were frozen at -80 ºC immediately after being retrieved and stored under this condition until initiating the proteomics analysis protocol. Glands were washed with cold PBS and immersed in 120 l of homogenization buffer (50 mM Tris-HCl, pH 6.8, 10 mM DTT, 4% (w/v) SDS). Glands were boiled for ...
ORGANISM(S): Trichonephila clavipes 
2022-12-07 | PXD023141 | Pride
Spider silk  proteomics  liquid chromatography-tandem mass spectrometry  silk gut 
MetabolomicsIdentification of pre-diagnostic lipid sets associated with liver cancer risk using untargeted lipidomics and chemical set analysis – a nested case-control study within the ATBC cohort

In pre-disposed individuals, a reprogramming of the hepatic lipid metabolism may support liver cancer initiation. We conducted a high-resolution mass spectrometry based untargeted lipidomics analysis of pre-diagnostic serum samples from a nested case-control study (219 liver cancer cases and 219 ...

2023-09-18 | MTBLS7776 | MetaboLights
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