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This study aimed to identify protein interaction partners of the lncRNA vA-202 using an RNA pull-down approach followed by mass spectrometry. Biotinylated vA-202 RNA was generated by in vitro transcription incorporating biotin-14-CTP. For each pull-down reaction, 400 µg of whole-cell lysate (in a to...
ORGANISM(S): Homo sapiens (Human) 
2026-05-25 | PXD070150 | Pride
mir-154 in mouse lung development - pull-down RNA
Long non-coding RNAs (lncRNAs) play critical regulatory roles in primate-specific biological processes, yet many primate-specific lncRNAs and their protein interactors remain poorly characterized. In this study, we focused on primate-specific lncRNAs as potential drivers of protein interactions. Usi...
ORGANISM(S): Homo sapiens (Human) 
2026-06-29 | PXD075354 | Pride
ACSL4mRNA probe was designed for pull-down experiments in HaCaT lysates
ORGANISM(S): Homo sapiens (Human) 
2025-10-13 | PXD052755 | Pride
RNA pull down assay of GST-ZFP36L1 (wildtype and mutant)
Antisense and sense probes of noncoding RNA GAS5 were used to perform the RNA pull down experiment on mouse hippocampus tissues. Then the proteins were identified by LC-MS/MS.
ORGANISM(S): Mus musculus (Mouse) 
2021-09-09 | PXD023363 | Pride
To screen Snhg3-binding proteins using-RNA Pull down and MS
ORGANISM(S): Mus Musculus 
2023-01-17 | PXD039526 |
RNA pull-down assay.
For the recombinant protein pull-down assays, 50 M-5g of recombinant His-tag TcRBP40 protein were bound to 100 uL of Ni-NTA resin (Qiagen) overnight at 4M-0C. 100 M-5g of total RNA from epimastigotes were incubated with the bound protein in 500 M-5l EMSA buffer at 4M-0C for 2...
ORGANISM(S): Trypanosoma cruzi 
Recent studies have revealed the importance of long noncoding RNAs (lncRNAs) as tissue-specific regulators of gene expression. There is ample evidence that distinct types of vasculature undergo tight transcriptional control to preserve their structure, identity, and functions. We determined, for the...
ORGANISM(S): Homo sapiens (Human) 
2020-12-21 | PXD018578 | Pride
We used circular RNA 101093 and its antisense to perform RNA pull-down, and analyzed the peptides in blank group, antisense group and circular RNA 101093 group. The object of this group is to analyze circ101093-binding protein. Blank and antisense group are used as negative control group.
ORGANISM(S): Homo sapiens (Human) 
2022-05-20 | PXD025575 | Pride
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