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To delineate the oncogenic mechanism of tRF-24 in ESCC, we performed RNA pull-down assays with biotinylated tRF-24 and antisense control, followed by mass spectrometry (MS) analysis.
ORGANISM(S): Homo sapiens (Human) 
2025-12-01 | PXD063163 | Pride
Goal was to identify RNA-binding proteins with a preference for m6A-modified RNA using an RNA bait generated from a known m6A site in the Dlg4 (PSD-95) mRNA 3`-UTR. By comparison of differential enrichment between m6A-RNA pulldown fractions and A-RNA or no RNA fractions, we can identify m6A-binding ...
ORGANISM(S): Mus musculus (Mouse) 
2022-10-13 | PXD034159 | Pride
Goal was to identify RNA-binding proteins with a preference for m6A-modified RNA using an RNA bait generated from a known m6A site in the Dlg4 (PSD-95) mRNA 3`-UTR. By comparison of differential enrichment between m6A-RNA pulldown fractions and A-RNA or no RNA fractions, we can identify m6A-binding ...
ORGANISM(S): Mus musculus (Mouse) 
2022-10-13 | PXD034161 | Pride
RNA pulldown target circGLS2
In order to explore the lncRNA Crnde interacting proteins in mouse diencephalon tissues, we first designed and synthesized Crnde sense and anti-sense mRNA probes, and collected the diencephalon tissues of embryonic mice for RNA pulldown experiment, and identified the enriched products by mass spectr...
ORGANISM(S): Mus musculus (Mouse) 
2026-02-14 | PXD056797 | Pride
As a newly identified mRNA modification, the regulation of ac4C remains largely unexplored. RNA-binding proteins (RBPs) that specifically binds to ac4C modification and mediate downstream cellular activities (readers) have not been reported yet. We synthesized acetylated and non-acetylated RNA probe...
ORGANISM(S): Homo sapiens (Human) 
2021-07-20 | PXD027014 | Pride
RNA sensing is critical for RLR activation and downstream signaling. To identify molecules that are involved in modulating viral RNA recognition, we utilized biotin-labeled 60 nt single-stranded RNA (ssRNA) from SARS-CoV-2 NSP1 coding region (NSP1 ssRNA) to pull down proteins from RAW264.7 cells. Th...
ORGANISM(S): Mus musculus (Mouse) 
2026-04-08 | PXD064808 | Pride
Gas5 Pulldown Total and small RNAseq
Vectors carrying lnc-GD2H and antisense lnc-GD2H were linearized with the corresponding restriction enzymes to prepare template DNAs for in vitro transcription. Biotinylated RNAs were mixed with proteins extracted from C2C12 cells, followed by targeting of the RNAs with streptavidin beads. After SDS...
ORGANISM(S): Mus musculus (Mouse) 
2021-05-18 | PXD025292 | Pride
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