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MetabolomicsEvaluation of sample preparation methods for NMR-based metabolomics of cow milk
The quality of milk metabolome analyzed by nuclear magnetic resonance (NMR) is greatly influenced by the way samples are prepared. Although this analytical method is increasingly used to study milk metabolites, a thorough examination of available sample preparation protocols for milk has not been re...
2019-06-13 | MTBLS623 | MetaboLights
MetabolomicsAssessment of human plasma and urine sample preparation for re-producible and high-throughput UHPLC-MS clinical metabolic phenotyping

Clinical metabolic phenotyping employs metabolomics and lipidomics to detect and measure thousands of metabolites and lipids within human samples. This approach aims to identify metabolite and lipid changes between phenotypes (e.g. disease status) that aid understanding of biochemical mechanisms ...

2020-08-06 | MTBLS1465 | MetaboLights
ProteomicsProtein identification in 1000 U2OS cells
In this study, we aim to optimize sample processing methods using a conventional in-solution proteomic sample processing workflow. We then compared protein idetnfication numbers withour improved sample processing methods versus the original method, in 1000 U2OS human osteosarcoma cells.
ORGANISM(S): Homo sapiens (Human) 
2019-05-06 | PXD013702 | Pride
Sample recovery yield 1000 cells Lc-ms U2os Sam ple processing Osteosarcoma 
ProteomicsEPURISp: Combining Enzymatic Digestion, Ultrafiltration, and Rapid In-situ Sample Purification for High-performance Proteomics
High-performance liquid tandem mass spectrometry (HPLC-MS) is widely employed for protein analysis in biological systems. However, conventional proteomic sample pre-treatment methods suffer from multiple steps and poor reproducibility. In this study, we introduce EPURISp (Enzymatic Digestion with Ul...
ORGANISM(S): Mus Musculus 
2023-07-29 | PXD044209 |
ProteomicsS-Trap is an ultrafast sample preparation approach for shotgun proteomics
The success of shotgun proteomic analysis depends largely on how samples are prepared. Current approaches such as gel-, solution- or filter-based, although being extensively employed in the field, are time-consuming and less effective with respect to the repetitive sample processing, recovery, and o...
ORGANISM(S): Escherichia coli 
2019-03-28 | PXD010237 | Pride
Proteomics  sample preparation  fasp  suspension trapping  klebsiella pneumoniae  sputum  tuberculosis. 
ProteomicsOptimised Sample Handling Minimises Peptide Adsorption to Plastics to Enable High Sensitivity Evosep Based Chemical Proteomics
Chemical proteomics approaches often yield low peptide recovery because they aim to enrich low-abundance proteins. Poor sample handling further reduces recovery through peptide adsorption to plastic surfaces and losses due to vacuum evaporation. We systematically mapped these losses across buffers, ...
ORGANISM(S): Homo sapiens (Human) 
2026-06-23 | PXD069322 | Pride
Evosep Optimisation Chemoproteomics 
UnknownEnhanced FASP (eFASP) to increase proteome coverage and sample recovery for quantitative proteomic experiments.
Not available
S-EPMC3993969 | biostudies-literature
ProteomicsSignal Attrition in Whole Cell Crosslinking Mass Spectrometry
We investigated points in the typical sample workflow that could contribute to the perceived low cross-linking yields. We demonstrate, through a 2-step cross-linking strategy called Click-Linking, that the chemical reaction is not the main contributor to the low number of identified cross-links. We ...
ORGANISM(S): Homo sapiens (Human) 
2025-11-18 | PXD069512 | Pride
Cross-linking Xl-ms Reaction yield 
TranscriptomicsRNA-seq of human prostate cancer cell line with IRF7 knock-down treated with radiation against wild-type control
Abstract/Project overview Tumour recurrence can be influenced by the mutational status of a tumour but also how the tumour responds to therapy. Whilst activation of Type 1 interferon signalling is a hallmark of how cells respond to viral infection, in cancer cells, multiple stresses are known to a...
ORGANISM(S): Homo sapiens 
2023-07-01 | E-MTAB-9286 | biostudies-arrayexpress
UnknownTenfold difference in DNA recovery rate: systematic comparison of whole blood vs. dried blood spot sample collection for malaria molecular surveillance.
Not available
S-EPMC8922754 | biostudies-literature
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