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ProteomicsSARS-CoV-2 Mpro subtiligase-mediated N-terminomics in A549
Subtiligase-based N-terminomics study with 0.5 uM of recombinant SARS-CoV-2 Mpro in A549 cell lysates, in duplicate experiments.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-12-17 | MSV000088583 | MassIVE
ProteomicsSARS-CoV-2 Mpro subtiligase-mediated N-terminomics in Jurkat
Subtiligase-based N-terminomics study with 0.5 uM SARS-CoV-2 Mpro in Jurkat cell lysates. Independent duplicates were injected with (L20200924-06, L20200924-07) and without FAIMS (L20200927-01-001, L20200927-02-002).
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-12-17 | MSV000088584 | MassIVE
ProteomicsN-terminomics for the identification of in-vitro substrates and cleavage site specificity of the SARS-CoV-2 main protease
The genome of coronaviruses, including the SARS-CoV-2, which causes the human pandemic COVID-19, encodes for two proteases, a papain like (PL) and the so-called Main protease (Mpro), also named 3CLpro or non-structural protein 5 (NSP5). Mpro is activated by autoproteolysis, and true to its name, is ...
ORGANISM(S): Homo sapiens (Human) 
2020-11-02 | PXD021406 | Pride
Sars-cov-2 Sars-cov N-terminomics Lcms Proteomics 
ProteomicsBiochemical and structural insights into SARS-CoV-2 polyprotein processing by Mpro (HDX-MS pulse labeling)
SARS-CoV-2, a human coronavirus, is the causative agent of the COVID-19 pandemic, entailing enormous disruption worldwide. Its ~30 kb RNA genome is translated into two large polyproteins subsequently cleaved by viral papain-like protease and main protease (Mpro/nsp5). Polyprotein processing is essen...
ORGANISM(S): Severe acute respiratory syndrome coronavirus 2 
2022-12-19 | PXD033702 | Pride
Pulse labeling Hdx-ms Mpro Sars-cov-2 Polyprotein 
ProteomicsBiochemical and structural insights into SARS-CoV-2 polyprotein processing by Mpro (HDX-MS continuous labeling)
SARS-CoV-2, a human coronavirus, is the causative agent of the COVID-19 pandemic, entailing enormous disruption worldwide. Its ~30 kb RNA genome is translated into two large polyproteins subsequently cleaved by viral papain-like protease and main protease (Mpro/nsp5). Polyprotein processing is essen...
ORGANISM(S): Severe acute respiratory syndrome coronavirus 2 
2022-12-19 | PXD033698 | Pride
Hdx-ms Mpro Sars-cov-2 Polyprotein Continuous labeling 
ProteomicsBiochemical and structural insights into SARS-CoV-2 polyprotein processing by Mpro (XL-MS)
SARS-CoV-2, a human coronavirus, is the causative agent of the COVID-19 pandemic, entailing enormous disruption worldwide. Its ~30 kb RNA genome is translated into two large polyproteins subsequently cleaved by viral papain-like protease and main protease (Mpro/nsp5). Polyprotein processing is essen...
ORGANISM(S): Severe acute respiratory syndrome coronavirus 2 
2022-12-19 | PXD033748 | Pride
Nsp7-8 Xl-ms Nsp7-11 Mpro Sars-cov-2 Polyprotein 
ProteomicsStatistical crystallography reveals an allosteric network in SARS-CoV-2 Mpro
To interpret and transmit biological signals, proteins use correlated motions. Experimental determination of these dynamics and the structural distributions they generate remains a key challenge. Here, using 1146 crystal structures of the main protease (Mpro) from SARS-CoV-2, we were able to infer a...
ORGANISM(S): Escherichia coli 
2026-04-14 | PXD076496 | Pride
Enzymology Sars-cov-2 Crystallography 
ProteomicsA yeast-based system to study SARS-CoV-2 Mpro structure and to identify nirmatrelvir resistant mutations
The SARS-CoV-2 main protease (Mpro) is a major therapeutic target. The Mpro inhibitor, nirmatrelvir, is the antiviral component of Paxlovid, an orally available treatment for COVID-19. As Mpro inhibitor use increases, drug resistant mutations will likely emerge. We have established a non-pathogenic ...
ORGANISM(S): Saccharomyces cerevisiae Caenorhabditis elegans 
2023-08-08 | PXD036325 | Pride
Covid-19 
ProteomicsExpedited SARS-CoV-2 MPro inhibitor discovery through a modular, 'direct-to-biology' approach
Reactive fragment (RF) screening has emerged as an efficient method for ligand discovery across the proteome, irrespective of a target’s perceived tractability. To date, however, the efficiency of subsequent optimisation campaigns has largely been low-throughput, constrained by the need for synthesi...
ORGANISM(S): Homo sapiens (Human) 
2024-12-20 | PXD056098 | Pride
Covalent drug discovery Chemoproteomics Sars-cov-2 mpro 
TranscriptomicsThe SARS-CoV-2 main protease Mpro causes microvascular brain pathology by cleaving NEMO in brain endothelial cells
COVID-19 can damage cerebral small vessels and cause neurological symptoms. However, the vascular pathology and the potential mechanisms are unclear. In brains of SARS-CoV-2-infected patients and animal models, we found an increased number of empty basement membrane tubes, so-called string vessels r...
ORGANISM(S): Mus musculus 
2021-07-31 | GSE180984 | GEO
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