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Proteomicsdichotomic size-exclusion chromatography（SEC）
directDIA proteomics raw data of plasma extracellular vesicles isolated by SEC and UC
ORGANISM(S): Homo Sapiens 
PXD026034 |
ProteomicsImpact of size exclusion chromatography on the purity and proteomic profiles of extracellular vesicles derived from Lactobacillus reuteri
Extracellular vesicles (EVs) produced by probiotic bacteria are increasingly recognized as key mediators of host-microbe communication. However, the molecular composition and biological interpretation of bacterial EV proteomes are strongly influenced by the isolation method used. Here, we systematic...
ORGANISM(S): Homo sapiens (Human) Bacteria 
2026-09-01 | PXD075819 | Pride
Membrane vesicles Exosomes Inflammation Lactic acid bacteria Limosilactobacillus 
ProteomicsProteomic profiling of subsets of NK cell-derived extracellular vesicles by LC-MS/MS
In this study we have compared the proteomic profile of subsets of extracellular vesicles (EVs) prepared from the human NK cell line NK-92 cultured for 48hrs in serum-free conditions supplemented with 10 ng/ml human recombinant IL-15. The aim was to isolate and define an EV subset with cytolytic act...
ORGANISM(S): Homo sapiens (Human) 
2022-09-09 | PXD034603 | Pride
Nk-92 Nk cells Dg-uc Il-15 Sec Lc-ms/ms 
ProteomicsComparative Analysis of Extracellular Vesicle Isolation from Equine Serum and Plasma
To compare the efficiency, reproducibility, and proteomic profiles of extracellular vesicles (EVs) isolated from equine serum and plasma using size-exclusion chromatography (SEC) and differential ultracentrifugation (UC)+SEC, and to assess how the starting material influences EV cargo. Blood was col...
ORGANISM(S): Equus caballus (Horse) 
2026-01-23 | PXD068077 | Pride
Evs Exploris 480 Horse Sp3 Evosep one Sec uc 
ProteomicsThe proteome of circulating extracellular vesicles and their functional effect on platelets vary with the isolation method
Extracellular vesicles (EVs) play a crucial role in cell-to-cell communication and serve as a source of biomarkers in several pathologies. In this study, we aimed to characterize plasma-derived EVs isolated by ultracentrifugation (UC) or size exclusion chromatography (SEC) to define the best method ...
ORGANISM(S): Homo sapiens (Human) 
2025-02-14 | PXD056613 | Pride
Platelets Ultracentrifugation Extracellular vesicles Size exclusion chromatography Proteomics. 
ProteomicsComparison of three methods for isolating human plasma EVs by LC-MSMS
The lack of standardized protocols for isolating extracellular vesicles (EVs), particularly from biobank-stored plasma, poses a substantial limitation for the study of biomarkers. Combining current isolation methods could enhance the specificity and purity of EVs isolation for their further use in d...
ORGANISM(S): Homo sapiens (Human) 
2024-07-03 | PXD049342 | Pride
Human Plasma Extracellular vesicles 
GenomicsHomo sapiens
Gene target specificity of the Super Elongation Complex (SEC) family: How HIV-1 Tat employs selected SEC members to activate viral transcription
PRJNA284330 | ENA
ProteomicsA comparison of blood plasma exosome enrichment strategies for proteomic analysis.
Proteomic analysis of exosomes (EXs) poses a significant challenge. A ‘gold-standard’ method for plasma EX enrichment for downstream proteomic analysis is yet to be established. Methods were evaluated for their capacity to successfully isolate and enrich EXs from plasma, minimise the presence of hig...
ORGANISM(S): Bos taurus (Bovine) 
2022-08-12 | PXD032313 | Pride
Exosome  isolation  enrichment  ultracentrifugation  size-exclusion chromatography  ultrafiltration  mass-spectrometry  proteomics  extracellular vesicles 
UnknownImproving the Purity of Extracellular Vesicles by Removal of Lipoproteins from Size Exclusion Chromatography- and Ultracentrifugation-Processed Samples Using Glycosaminoglycan-Functionalized Magnetic Beads.
Not available
S-EPMC11367580 | biostudies-literature
ProteomicsUrinary exosomes identification
Urinary exosomes extracted with SEC and UC methods. Several exosomal fractions were collected during subsequent separation by SEC column with 2000 Å packing. lytic exosomal proteins were reduced with DTT and digested with trypsin. LC-MS/MS was used for exosomal proteins identification
ORGANISM(S): Homo Sapiens 
2020-03-24 | PXD016646 |
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