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Analysis of HeLa cells at 24 hours after transfection with wild type miR-1, miR-124, miR-181 versus control transfected HeLa cells. Results were compared to protein down-regulation at 48 hours measured by SILAC-MS. Analysis of HeLa cells at 24 hours after transfection with wild type miR-1, miR-124,...
ORGANISM(S): Homo sapiens 
in this study, we performed the SILAC-MS to identify H2AK119ub1 specific binding proteins.Samples were analyzed on a Q Exactive equipped with an Easy n-LC 1000 HPLC system (Thermo Scientific)
ORGANISM(S): Homo sapiens (Human) 
2020-02-06 | PXD017105 | Pride
Current understanding of oocyte quality and developmental potential maintains that stochastic or epigenetic processes modulate the action of determinants that are laid in the oocyte during oogenesis. These determinants are considered to be rather conserved across mice, leading different strains of m...
ORGANISM(S): Mus musculus 
The DNA-damage response (DDR) is a critical network for maintaining genomic integrity, and mutations in the DDR are among the most frequently identified in tumors. Phosphorylation is a key signaling event in the DDR network. We sought to design high throughput, mass spectrometry based assays to moni...
ORGANISM(S): Homo sapiens (Human) 
2015-12-01 | PXD002363 | Pride
SILAC-based metabolic labeling is a widely adopted proteomics approach that enables quantitative comparisons among a variety of experimental conditions. Despite its quantitative capacity, SILAC experiments analyzed with data dependent acquisition (DDA) do not fully leverage peptide pair information ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-11-03 | PXD033016 | Pride
abundance based on SILAC normalized data, MS/MS abundance data, Interaction consistency score: 15.7, Coverage: 60
ORGANISM(S): 4932 
2008-00-00 | 469118354 | PAXDB
Hepatitis B virus (HBV) infection could cause hepatitis, liver cirrhosis and hepatocellular carcinoma. HBV-mediated pathogenesis is only partially understood, but X protein (HBx) reportedly possesses oncogenic potential. Exosomes are small membrane vesicles with diverse functions released by various...
ORGANISM(S): Homo sapiens (Human) 
2014-10-09 | PXD001339 | Pride
TET1 maintains hypomethylation at bivalent promoters through its catalytic activity in embryonic stem cells (ESCs). However, whether and how TET1 exerts catalytic activity-independent functions in regulating bivalent genes is not well understood. Therefore, we mapped the TET1 interactome in mouse ES...
ORGANISM(S): Mus musculus (Mouse) 
2022-06-10 | PXD033587 | Pride
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