Sort   by:  
 Page size 
MetabolomicsIntegrating two chromatographies in a co-fractionation mass spectrometry approach improved protein-metabolite interactions (PMIs) mapping capabilities and revealed novel protein-ligand pairings in Escherichia coli.
The protein-metabolite interactome remains understudied. We previously developed a co-fractionation mass-spectrometry-based approach called PROMIS to address this gap. PROMIS relies on size separation chromatography of protein-metabolite complexes from lysates, followed by proteomics and metabolomic...
2025-04-25 | MTBLS9098 | MetaboLights
MetabolomicsPROMIS, global analysis of PROtein-Metabolite Interactions using Size separation in Arabidopsis thaliana
Small molecules not only represent cellular building blocks and metabolic intermediates, but also regulatory ligands and signaling molecules that interact with proteins. Although these interactions affect cellular metabolism, growth, and development, they have been largely understudied. Herein, we d...
2018-07-19 | MTBLS94 | MetaboLights
TranscriptomicsResponses of Arabidopsis leaves to prolonged osmotic stress are mediated by their developmental stage
Drought is an important environmental factor affecting plant growth and biomass production. Despite this importance, little is known on the molecular mechanisms regulating plant growth under water limiting conditions. The main goal of this work was to investigate, using a combination of growth and m...
ORGANISM(S): Arabidopsis thaliana 
2010-04-11 | E-GEOD-16474 | biostudies-arrayexpress
ProteomicsDipeptide Tyr-Asp inhibits glyceraldehyde 3-phosphate dehydrogenase activity, affecting redox metabolism and boosting plant stress resistance.
Proteogenic dipeptides are not only intermediates of proteolysis but also an emerging class of small molecule regulators, with diverse and specific functions. We have recently reported a novel in vitro interaction between the dipeptide Tyr-Asp and the Arabidopsis glycolytic enzyme glyceraldehyde 3- ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2021-05-10 | PXD019332 | Pride
Pepck Dipeptides Arabidopsis Gapdh Tyr-asp Oxidative stress Glycolysis 
ProteomicsCharacterization of the cyclic dipeptide cyclo(His-Pro) in Arabidopsis
The iTSA experiment was designed to map putative protein targets of cyclic dipeptides in Arabidopsis.
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2025-04-17 | MSV000097655 | MassIVE
ProteomicsA global map of protein–metabolite interactions in budding yeast reveals a novel role of a proteogenic dipeptide Ser-Leu in the regulation of a key glycolytic enzyme Pgk1.
Protein-metabolite interactions (PMIs) are of crucial importance for all cellular processes but remain understudied. Here, we applied a novel biochemical approach named PROMIS, to address the complexity of the protein-small molecule interactome in the model yeast Saccharomyces cerevisiae. By doing s...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-04-26 | PXD021530 | Pride
Complexes Dipeptide Yeast Pgk1 Small molecule Promis Ligand Protein–metabolite interaction Glycolysis Central metabolism Purine degradation 
ProteomicsA multi-OMICs approach sheds light on the higher yield phenotype and enhanced abiotic stress tolerance in tobacco lines expressing the carrot lycopene β-cyclase1 gene
Here, we combine OMICs (RNAseq, proteomics, and metabolomics) approaches to advance our understanding of the broader effect of LCYB expression on the tobacco genome and metabolism. DcLCYB1 gene with accelerated development, increased carotenoid content, photosynthetic efficiency, and yield might be ...
ORGANISM(S): Nicotiana tabacum (Common tobacco) 
2021-09-10 | PXD023595 | Pride
High light and salt stress Omics Β-carotene Ros Nicotiana tabacum cv xanthi Lycopene β-cyclase Transcription factors Carotenoids 
ProteomicsProtein and metabolite composition of Arabidopsis stress granules
• Stress granules (SGs) are evolutionary conserved aggregates of proteins and untranslated mRNAs formed in response to stress. Despite their importance for stress adaptation, no complete proteome composition has been reported for plant SGs. Herein, we addressed the existing gap. Importantly, we also...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-01-07 | PXD011716 | Pride
Cdka1 Arabidopsis thaliana Stress granules proteome Stress response Stress granule metabolome Affinity purification Stress granule extraction 
ProteomicsIdentification and characterization of the heat-induced plastidial stress granules-like foci in Arabidopsis thaliana.
Plant cells, similar to animal cells repress translation under stressful conditions which leads to formation of cytosolic stress granules (SGs). Here, we report that in addition to cytosolic SGs plants also have plastidial SGs (pSGs). In order isolate and determine protein and metabolite composition...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-09-24 | PXD018348 | Pride
Stress granules Arabidopsis thaliana Co-ip Interactions Ap_ms Snowy cotyledon 1. stress treatment 
Proteomics2′ ,3′-cAMP treatment mimics abiotic stress response
For many years, 2′,3′-cyclic adenosine monophosphate (2′,3′-cAMP), a positional isomer of the second messenger 3′,5′-cAMP, has not received enough attention. Recent studies have reported that 2′,3′-cAMP exists in plants and might be involved in stress signaling because 1) its level increases upon wo...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-05-19 | PXD028365 | Pride
2′ Abiotic stress 3′-camp Stress granules Stress response Processing bodies 
Sort   by:  
 Page size