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ProteomicsStructural and functional analyses identify unique features of the Smc5/6 complex among SMC family of complexes
Cross-linking mass spectrometry data for integrative structural analyses of the Smc5/6 holo-complex from budding yeast. Electron microscopy, crosslinking mass spectrometry, and computational modeling reveal that while the complex shares an overall organization with other SMC complexes, it possesses ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-05-05 | PXD023164 | Pride
Smc5/6 Cross-linking Yeast Integrative modeling Xl-ms Proteomics 
TranscriptomicsSMC5/6 facilitates joint molecule resolution and stepwise loss of cohesin to promote meiotic chromosome segregation
During meiosis, Structural Maintenance of Chromosome (SMC) complexes underpin two fundamental features of meiosis: homologous recombination and chromosome segregation. While meiotic functions of the cohesin and condensin complexes have been delineated, the role of the third SMC complex, Smc5/6, rema...
ORGANISM(S): Saccharomyces cerevisiae 
2013-12-27 | E-GEOD-44852 | biostudies-arrayexpress
ProteomicsPurified Smc5/6 complex exhibits DNA substrate recognition and compaction
Investigation into the topology and fucntion of the yeast SMC5/6 complex
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-02-10 | PXD016196 | Pride
Crosslinking bs3 smc5/6 complex 
ProteomicsThe Human Nse5 Orthologue SIMC1 Localizes SMC5/6 to Polyomavirus Replication Centers
The human SMC5-SMC6 complex (SMC5/6) is a conserved guardian of genome stability and an emerging component of the antiviral response in animals and plants. In yeast, Smc5/6 is recruited to and loaded on chromatin by the poorly conserved Nse5-Nse6 heterodimer (Nse5/6), which remains largely undefined...
ORGANISM(S): Homo sapiens (Human) 
2022-11-29 | PXD033923 | Pride
Nse5 Lcms Simc1 localizes smc5/6 
ProteomicsRole of Nse1 subunit of SMC5/6 complex as a ubiquitin ligase
Structural Maintenance of Chromosomes (SMC) complexes - cohesin, condensin and SMC5/6 - are important for many aspects of the chromosomal organization. These complexes are composed of Smc and non-Smc element (Nse) subunits. The Nse1 subunit of SMC5/6 contains a variant RING domain, characteristic of...
ORGANISM(S): Homo sapiens (Human) Schizosaccharomyces pombe 927 
2022-01-24 | PXD029573 | Pride
Liquid chromatography Mass spectrometry Ubiquitination Smc5/6  nse1 Ubiquitin ligase 
TranscriptomicsChromosomal association of the Smc5/6 complex reveals that it functions in differently regulated pathways
The SMC protein complexes safeguard genomic integrity through their functions in chromosome segregation and repair. The chromosomal localization of the budding yeast Smc5/6 complex here determined reveals that the complex works specifically on the duplicated genome in differently regulated pathways....
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-11 | E-GEOD-4808 | biostudies-arrayexpress
ProteomicsNse5/6 inhibits the Smc5/6 ATPase and modulates DNA substrate binding
Eukaryotic cells employ three SMC complexes to control DNA folding and topology. The Smc5/6 complex plays roles in DNA repair and in preventing the accumulation of deleterious DNA junctions. To elucidate how specific features of Smc5/6 govern these functions, we reconstituted the yeast holo-complex....
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-07-02 | PXD024160 | Pride
Atpase Condensin Loop extrusion Chromosome segregation Xl-ms Smc5 Smc Smc6 Genome maintenance Cohesin 
TranscriptomicsEssential Roles of the Smc5/6 complex in Replication through Pausing Sites and Endogenous DNA Damage Tolerance
The essential functions of the conserved Smc5/6 complex remain elusive. To uncover its roles in genome maintenance, we established Saccharomyces cerevisiae cell cycle-regulated alleles that enable restriction of Smc5/6 components to S or G2/M. Unexpectedly, the essential functions of Smc5/6 segregat...
ORGANISM(S): Saccharomyces cerevisiae 
2015-11-19 | E-GEOD-72241 | biostudies-arrayexpress
GenomicsNse4-FLAG CHIP-seq analysis in gcn5, ubp8, ada2 deletion background in S.pombe, control wt 501 - no tag
to find functional relevance between SMC5/6 complex and SAGA histone acetyltransferase complex in Schizosaccharomyces pombe by chromatin immunoprecipitation of Nse4-FLAG tagged protein from gcn5, ubp8 and ada2 deletion strains and 2 control strains (WT - Nse4-FLAG, 501 - no tag).
ORGANISM(S): Schizosaccharomyces pombe 
2023-02-20 | E-MTAB-12401 | biostudies-arrayexpress
GenomicsHuman Smc5/6 recognizes transcription-generated positive DNA supercoils
Human Smc5/6 recognizes transcription-generated positive DNA supercoils
PRJNA963774 | ENA
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