Spatially resolved proteomics

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Technology Type

Mass Spectrometry (106)
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Top-down proteomics (6)
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HDMSE (1)
SRM/MRM (1)
Chemical cross-linking coupled with mass spectrometry proteomics (1)

Instrument Platform

LTQ Orbitrap Velos (6)
Q Exactive Plus (6)
GC MS (4)
GC-MS (3)
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6520A Quadrupole Time-of-Flight LC/MS (1)
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2026 (5)
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Release Date

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Lab affiliation

Division of Molecular and Cellular Function School of Biological Science Faculty of Biology Medicine and Health The University of Manchester Manchester United Kingdom (3)
Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany (2)
hong kong baptist university (2)
Dalian Institute of Chemical Physics (2)
Université de Lille, Sciences et Technologies - INSERM U1192 - Laboratoire PRISM (2)
Director of Women’s Health Research Women’s Service Line Inova Health System (2)
Discovery Proteomics Facility, University of Oxford (2)
Zhengzhou University (2)
University of Hohenheim, Institute of Animal Science, Stuttgart, Germany (2)
Pacific Northwest National Laboratory (2)
Systems Biology Ireland, University College Dublin (2)
Center for Thoracic Cancers, MGH Cancer Center Massachusetts General Hospital Harvard Medical School USA (1)
Department of Immunology and Regenerative Biology, Weizmann Institute of Science, Rehovot, Israel (1)
1. Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Copenhagen, Denmark; 2. Proteomics and Singal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany (1)
Assistant Professor of Microbiology, Immunology, and Cancer Biology Associate Director for Diversity, Equity, and Inclusio at the Carter Immunology Center University of Virginia Charlottesville, VA (1)
1Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, Roosevelt Drive, Oxford, OX3 7FZ, UK (1)
Division of Cell Matrix Biology and Regenerative Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester Academic Health Science Centre, Manchester, M13 9PL, UK. (1)
Univ.Lille, Inserm, CHU Lille, U1192 - Protéomique Réponse Inflammatoire Spectrométrie de Masse - PRISM, F-59000 Lille, France (1)
Professor Department of Biology University of York York United Kingdom (1)
Stress- and Immunoproteomics Group, Molecular and Applied Microbiology Department, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute Jena (1)
King's College London School of Cardiovascular and Metabolic Medicine & Sciences James Black Centre 125 Coldharbour Lane, London, SE5 9NU (1)
1. Center for Translational Biomedical Research, University of North Carolina at Greensboro 2. Department of Chemistry & Biochemistry, University of North Carolina at Greensboro (1)
Sch1School of Pharmacy, Department of Chemistry,University of Wisconsin-Madison, Madison, Wisconsin, 53705, (1)
Discovery Proteomics Lab, University of Oxford (1)
Division of Vascular Oncology and Metastasis Research,German Cancer Research Center (DKFZ), Heidelberg, Germany. European Center for Angioscience (ECAS), Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany. (1)
Spatial Proteomics Max Delbrück Center Robert-Rössle-Straße 10 13125 Berlin, Germany (1)
Division of Vascular Oncology and Metastasis Research, German Cancer Research Center (DKFZ), Heidelberg, Germany. European Center for Angioscience (ECAS), Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany. (1)
Center for Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied Sciences, Paul-Wittsack Str. 10, 68163, Mannheim, Germany. (1)
Department of Pharmaceutical Sciences, University of Illinois College of Pharmacy,Chicago,IL,USA Richard and Loan Hill Department of Biomedical Engineering, University of Illinois College of Engineering,Chicago, IL,USA University of Illinois Cancer Center,Chicago, IL,USA (1)
Dept. Proteomics and Signal Transduction Max Planck Institute of Biochemistry Am Klopferspitz 18 82152 Martinsried (near Munich) Germany (1)

Publication date

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2021 (13)
2016 (12)
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2020 (5)
2018 (4)
2015 (3)
2017 (2)

Tags

xref:PubMed:40295505 (3)
xref:PubMed:38234840 (3)
xref:PubMed:38897168 (2)
xref:PubMed:41862629 (2)
xref:PubMed:34038707 (1)
xref:PubMed:31535084 (1)

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Spatially Resolved Proteomics via Tissue Expansion

Spatially resolved proteomics is an emerging approach, however, it remains technically challenging due to the complexity of the tissue microsampling techniques and mass spectrometry analysis of nanoscale specimen volumes. Here, we describe an expansion proteomics method ProteomEx, a combination of t...

ORGANISM(S): Mus <genus>

2022-11-04 | PXD037950 |

Spatially resolved proteome profiling of less than 200 cells from tomato fruit pericarp by integrating laser-capture microdissection with nanodroplet sample preparation

Proteomic data from technical developments of a nanoPOTS-based spatially resolved proteome profiling of <200 cells from tomato fruit pericarp.

ORGANISM(S): Solanum lycopersicum

2018-08-21 | PXD010806 | Pride

On-tissue spatially-resolved glycoproteomics guided by N-glycan imaging reveal global dysregulation of canine glioma glycoproteomic landscape

Here we present an approach to identify N-linked glycoproteins and deduce their spatial localization using a combination of MALDI N-glycan MSI and spatially-resolved glycoproteomics. We subjected glioma biopsies to on-tissue PNGaseF digestion and MALDI-MSI and found that the glycan HexNAc4-Hex5-NeuA...

ORGANISM(S): Canis familiaris (Dog) (Canis lupus familiaris)

2022-05-04 | PXD025537 | Pride

Glioblastoma Spatially resolved glycoproteomics Glycoproteomics Glycan imaging Haptoglobin Sialic acid Spatially resolved glycoproteomicsglycan imaging Maldi-msi Hypersialylation

Spatially resolved proteomics via tissue expansion.

Not available

S-EPMC9712279 | biostudies-literature

Laser capture microdissection-capillary zone electrophoresis-tandem mass spectrometry (LCM-CZE-MS/MS) for spatially resolved top-down proteomics: a pilot study of zebrafish brain

Mass spectrometry (MS)-based spatially resolved top-down proteomics (TDP) of tissues is crucial for understanding the roles played by microenvironmental heterogeneity in the biological functions of organs and for discovering new proteoform biomarkers of diseases. There are few published spatially re...

ORGANISM(S): zebrafish metagenome

2023-03-11 | PXD027741 | Pride

Spatially resolved top-down proteomics capillary zone electrophoresis-tandem mass spectrometry laser capture microdissection zebrafish brain proteoform high sensitivity neuropeptide hormone activity motor activity

On-tissue spatially-resolved glycoproteomics guided by N-glycan imaging reveal global dysregulation of canine glioma glycoproteomic landscape

ORGANISM(S): Canis lupus

2023-03-11 | PXD025826 | Pride

Glioblastoma Spatially-resolved Glycoproteomics Haptoglobin Sialic-acid Maldi-msi Hypersialylation Glycan-imaging

Spatially resolved top-down proteomics of tissue sections based on a microfluidic nanodroplet sample preparation platform

We demonstrate spatially resolved top-down proteomics (TDP) for proteoform identification and quantification from small sections of rat brain. We used the nanoPOTS (nanodroplet Processing in One pot for Trace Samples) platform, further improving the sample preparation by adding benzonase in the extr...

ORGANISM(S): Rattus Norvegicus (ncbitaxon:10116) Homo Sapiens (ncbitaxon:9606)

2022-03-30 | MSV000089163 | MassIVE

Spatially and cell-type resolved quantitative proteomic atlas of healthy human skin

Human skin provides both physical integrity and immunological protection from the external environment, using functionally distinct layers, cell types and extracellular matrix. Despite its central role in human health and disease, the constituent proteins of skin have not been systematically charact...

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

2021-10-15 | MSV000088236 | MassIVE

Spatially-Resolved Top-down Proteomics Bridged to MALDI MS Imaging Reveals the Molecular Physiome of Brain Regions.

Not available

S-EPMC5795397 | biostudies-literature

Chemical tools for temporally and spatially resolved mass spectrometry-based proteomics.

Not available

S-EPMC3925203 | biostudies-literature

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