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ProteomicsTube-gel digestion: a fast and reproducible sample preparation protocol for label-free quantitative proteomics of complex biological samples
Comparison of tube-gel protocol to stacking gel and liquid digestion for label-free extracted ion-current quantification of complex biological samples Dataset with known fold-changes (UPS1 spiked in yeast background at 25-10-5-2.5-1-0.5 fmol)
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2016-10-18 | PXD003841 | Pride
Label-free quantitative proteomics Liquid digestion Sample preparation Tube-gel Stacking gel 
ProteomicsHit-Gel: Streamlining in-gel protein digestion for high-throughput proteomics experiments.
Traditional in gel-digestion procedures require extensive sample handling, are prone to contamination and not compatible with high-throughput sample preparation. To address these shortcomings, we have modified the conventional in-gel digestion procedure for high-throughput proteomics studies. The mo...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-10-16 | PXD009485 | Pride
Large-scale In-gel digestion Proteomics Tryptic digest Fractionation Native protein complex Sample preparation High-throughput Lc-ms/ms 
ProteomicsHit-Gel: Streamlining in-gel protein digestion for high-throughput proteomics experiments.
Traditional in gel-digestion procedures require extensive sample handling, are prone to contamination and not compatible with high-throughput sample preparation. To address these shortcomings, we have modified the conventional in-gel digestion procedure for high-throughput proteomics studies. The mo...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-10-16 | PXD009510 | Pride
Large-scale In-gel digestion Proteomics Tryptic digest Fractionation Native protein complex Sample preparation High-throughput Lc-ms/ms 
ProteomicsComparative Two-Dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE) of Human Milk to Identify Dysregulated Proteins in Breast Cancer
Breast Cancer (BC) remains a major cause of mortality, and early detection is considered important for reducing BC-associated deaths. Early detection of BC is challenging in young women, due to the limitations of mammography on the dense breast tissue of young women. We recently reported results of ...
ORGANISM(S): Homo sapiens (Human) 
2018-06-25 | PXD009860 | Pride
Mass spectrometry Breast cancer Breast milk 2d-page Proteomics Biomarkers 
ProteomicsThe HAPSTR2 retrogene buffers stress signaling and resilience in mammals
Standard anti-FLAG magnetic beads for co-immunoprecipitations, elution in 2x SDS sample buffer, loaded into a stacking gel and then in-gel digested
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD038642 | Pride
Hapstr2 Hapstr1 Huwe1 
UnknownA tailored graphene supramolecular gel for pharmaceutical crystallization.
Not available
S-EPMC11949076 | biostudies-literature
UnknownSelf-Regulating Hydrogel with Reversible Optical Activity in Its Gel-to-Gel Transformation.
Not available
S-EPMC12100726 | biostudies-literature
ProteomicsGel based extracellular matrix proteome of jute
Extracellular matrix (ECM) is the first line of defense which is an inimitable organelle that perceives biotic and abiotic stresses and reprograms biological processes of host. It also activates innate immune responses in temporal and spatial manner and acts as physical scaffold that prevents the en...
ORGANISM(S): Corchorus olitorius 
2024-05-21 | PXD046939 | Pride
1-de sds page Mass spectrometry Jute Extracellular matrix 
ProteomicsProteomics analysis of human breast milk by two-dimensional polyacrylamide gel electrophoressi (2D-PAGE) coupled with mass spectrometry to assess breast cancer risk
Breast Cancer (BC) is one of the most common cancers and one of the most common causes for cancer- related mortality. Discovery of protein biomarkers associated with cancer, is considered as important for early diagnosis and prediction of the cancer risk. Protein biomarkers could be investigated by ...
ORGANISM(S): Homo sapiens (Human) 
2023-10-20 | PXD040188 | Pride
Breast cancer  breast milk  biomarkers  protein dysregulation  2d-page  proteomics  mass spec-trometry 
UnknownConstructing ROS-Responsive Supramolecular Gel with Innate Antibacterial Properties.
Not available
S-EPMC10458117 | biostudies-literature
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