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TranscriptomicsA native chromatin purification system for epigenomic profiling in C. elegans
We describe an in vivo chromatin purification system for genome-wide epigenetic profiling in C. elegans. In this system, we coexpressed the E. coli biotin ligase enzyme (BirA), together with the C. elegans H3.3 gene fused to BioTag, a 23-amino acid peptide serving as a biotinylation substrate for Bi...
ORGANISM(S): Caenorhabditis elegans 
2010-05-19 | E-GEOD-18898 | biostudies-arrayexpress
ProteomicsA novel inducible retroviral expression system for StrepHA-tandem affinity purification mass-spectrometry-based proteomics
Tandem-affinity purification mass spectrometry using the streptavidin-hemagglutinin (SH)-tag has been successfully employed to map signaling networks in several large-scale studies. However its application has so far been restricted to the small number of Flp/FRT-recombination competent cell lines....
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2016-01-05 | PXD002855 | Pride
Tandem affinity purification-mass spectrometry Protein-protein interaction Streptavidin-hemagglutinin-tag 
ProteomicsProtease-resistant streptavidin for interaction proteomics
Streptavidin-mediated enrichment is a powerful strategy to identify biotinylated biomolecules and their interaction partners by mass spectrometry. Since contamination by streptavidin limits protein identification, we here present protease-resistant streptavidin beads (prS). Their application to diff...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2020-05-27 | PXD016576 | Pride
Streptavidin Affinity purification Mass spectrometry Dna pull down Chip sicap Lipid binding protein Bioid Membrane protein Chip Biotin 
UnknownIsolation of Endogenously Assembled RNA-Protein Complexes Using Affinity Purification Based on Streptavidin Aptamer S1.
Not available
S-EPMC4613318 | biostudies-literature
ProteomicsChemical acetylation of ligands and two-step digestion protocol for reducing co-digestion in affinity purification-mass spectrometry
We present an effective, fast and user-friendly method to reduce co-digestion of bead-bound ligands, such as antibodies or streptavidin, in affinity purification-mass spectrometry experiments. A short pre-incubation of beads with Sulfo-NHS-Acetate leads to chemical acetylation of lysine residues, ma...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2023-10-23 | PXD043709 | Pride
Chemical acetylation Immunopurification Streptavidin Ligand Affinity purification Sulfo-nhs-acetate Nanobody Gfp 
ProteomicsProtease-resistant streptavidin for interaction proteomics
Streptavidin-mediated enrichment is a powerful strategy to identify biotinylated biomolecules and their interaction partners by mass spectrometry. Since contamination by streptavidin limits protein identification, we here present protease-resistant streptavidin beads (prS). Their application to diff...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2025-04-08 | PXD015858 | Pride
Lipid Mass spectrometry Interactors Streptavidin Membrane proteins Chip-sicap Biotinylation Bioid Chip Ceramide-binding proteins 
UnknownEngineering Streptavidin and a Streptavidin-Binding Peptide with Infinite Binding Affinity and Reversible Binding Capability: Purification of a Tagged Recombinant Protein to High Purity via Affinity-Driven Thiol Coupling.
Not available
S-EPMC4583386 | biostudies-literature
ProteomicsProximity labelling and cryomill affinity purification compared for Trypanosoma brucei proteins with unconfined, semi-confined and confined localisation
Identification of protein-protein interactions is a major contributor for understanding protein function and elucidating molecular and cellular mechanisms. Two strategies are currently popular for identification of protein interaction partners directly from the cellular environment. Affinity purific...
ORGANISM(S): Trypanosoma brucei 
2022-11-30 | PXD031245 | Pride
Cryomilling Affinity purification Proteome Bioid Interactome 
ProteomicsH4K20me2 SILAC Nucleosome Affinity Purification
Goal of this project was the identification of chromatin interacting proteins whose binding is differentially regulated by di-methylation of lysine 20 on histone H4 (H4K20me2). To achieve this unodified and H4K20me2-modified histone H4 were generated by native chemical ligation and assembled into re...
ORGANISM(S): Homo sapiens (Human) 
2019-02-27 | PXD009281 | Pride
H4k20me2 H4k20me0 Snap Silac Nucleosome affinity purification 
GenomicsBenchmark study of different chromatin isolation methods using the E2Fa transcription factor
Genome-wide identification of transcription factor (TF) binding sites is pivotal to our understanding of gene expression regulation. Although much progress has been made in the determination of potential binding regions of proteins by chromatin immunoprecipitation (ChIP), this method has some inhere...
ORGANISM(S): Arabidopsis thaliana 
2014-02-04 | E-GEOD-53422 | biostudies-arrayexpress
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