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MetabolomicsDoes Storage Post Sampling Influence the Stability of Sebum when used for Mass Spectrometry Metabolomics Analysis?

Sebum is a biofluid excreted by sebaceous glands in the skin. In recent years sebum has been shown to contain endogenous metabolites diagnostic of disease, with remarkable results for Parkinson’s Disease. Given that sebum sampling is facile and non-invasive, its potential for use in clinical bioc...

2025-07-15 | MTBLS7946 | MetaboLights
ProteomicsExperimental strategies to improve drug-target identification in mass spectrometry-based thermal stability assays
Mass spectrometry (MS)-based thermal stability assays have recently emerged as one of the most promising solutions for the identification of protein-ligand interactions. Here, we have investigated eight combinations of several recently introduced MS-based advancements, including the Phase-Constraine...
ORGANISM(S): Homo sapiens (Human) 
2023-05-10 | PXD040284 | Pride
Cetsa Mass spectrometry Tpp Target identification Thermal stability assays Drug-protein interactions 
ProteomicsExperimental strategies to improve drug-target identification in mass spectrometry-based thermal stability assays
In this study, we developed a model system for the assessment of small molecule-protein interactions using intact cells treated with a commercially available highly specific mitogen-activated protein kinase (MEK) 1/2 inhibitor to prepare a set of unfractionated test samples labeled with tandem mass ...
ORGANISM(S): Homo sapiens (Human) 
2023-05-10 | PXD034087 | Pride
Cetsa Mass spectrometry Tpp Target identification Thermal stability assays Drug-protein interactions 
ProteomicsLarge-scale characterization of drug-induced changes in thermal stability using proteome-wide thermal shift assays
In response to an ever-increasing demand of new small molecules therapeutics, numerous chemical and genetic tools have been developed to interrogate compound mechanism of action. Owing to its ability to characterize compound-dependent changes in thermal stability, the proteome-wide thermal shift ass...
ORGANISM(S): Homo sapiens (Human) 
2025-02-13 | PXD048009 | Pride
Mechanism of action Drug development 
ProteomicsLarge-scale characterization of drug mechanism of action using proteome-wide thermal shift assays - Revisions
In response to an ever-increasing demand of new small molecules therapeutics, numerous chemical and genetic tools have been developed to interrogate compound mechanism of action. Owing to its ability to characterize compound-dependent changes in thermal stability, the proteome-wide thermal shift ass...
ORGANISM(S): Homo sapiens (Human) 
2025-02-13 | PXD053138 | Pride
Thermal shift assay Proteomics Drug discovery 
ProteomicsDECODING PROTEIN METHYLATION FUNCTION WITH THERMAL STABILITY ANALYSIS
Protein methylation is emerging as an important modification beyond epigenetics. However, systems-wide analyses of protein methylation function lag behind compared to other modifications. Recently, thermal stability analyses have been developed which provide a proxy of a protein functional status. H...
ORGANISM(S): Mus musculus (Mouse) 
2023-04-27 | PXD038939 | Pride
Thermal stability Protein methylation Mouse embryonic stem cells Prmt5 Ezh2 Pisa 
ProteomicsChemoproteomics validates selective targeting of Plasmodium M1 alanyl aminopeptidase as a cross-species strategy to treat malaria
All current treatments for malaria are threatened by drug resistance, and new drug candidates that act via novel mechanisms are urgently needed. Here, we describe MIPS2673, an inhibitor of the Plasmodium M1 alanyl metalloaminopeptidase, which displays excellent in vitro antimalarial activity with no...
ORGANISM(S): Homo sapiens (Human) Plasmodium falciparum (isolate 3D7) 
2024-06-16 | PXD044125 | Pride
Malaria Lip-ms Thermal stability proteomics Plasmodium falciparum 
UnknownPOT1 stability and binding measured by fluorescence thermal shift assays.
Not available
S-EPMC8009405 | biostudies-literature
ProteomicsSystem-wide identification of enzyme substrates by thermal analysis (SIESTA)
Despite the immense importance of enzyme-substrate reactions, there is a lack of generic and unbiased tools for identifying the molecular components participating in these reactions on a cellular level. Here we developed a universal method called System-wide Identification of Enzyme Substrates by Th...
ORGANISM(S): Homo sapiens (Human) 
2021-01-11 | PXD014445 | Pride
Proteomics Post-translational modification Target Thermal stability Lc/ms Akt1 Trxr1 Parp10 Artd10 Rac-alpha serine threonine protein kinase Txnrd1 
ProteomicsSystem-wide identification of enzyme substrates by thermal analysis (SIESTA)
Despite the immense importance of enzyme-substrate reactions, there is a lack of general and unbiased tools for identifying the molecular components participating in these reactions on a cellular level. Here we developed a universal method called System-wide Identification of Enzyme Substrates by Th...
ORGANISM(S): Homo sapiens (Human) 
2021-01-11 | PXD010554 | Pride
Poly-(adp-ribose) polymerase-10 Post-translational modification Target Thioredoxin reductase 1 Artd10 Thermal stability Trxr1 Parp10 Marylation Substrate 
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