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ProteomicsEnvironmental stress affects the activity of metabolic and growth factor signaling networks and induces autophagy markers in breast cancer cell lines
The breast cancer cell line MCF7 was exposed to RT comditions for 15min, 2h or left in the incubator. Cells were harvested and lyzed. Samples were digested with trypsin and subjected to phosphoenrichment usin TiO2. Phosphopeptides were run in a LTQ-Orbitrap-XL. 4 biological replicates per condition ...
ORGANISM(S): Homo sapiens (Human) 
2014-04-11 | PXD000472 | Pride
Label free Mcf7 Room temperature exposition Tio2 phosphoenrichment 
ProteomicsKinase Substrate Enrichment Analysis Provides Insights into the Heterogeneity of Signaling Pathway Activation in Leukemia Cells
Experiments were performed in AML (Acute Myeloid Leukemia) cell lines and in primary cells coming from patients with AML and healthy donors. The AML cell lines P31/Fuj and Kasumi-1 were treated with vehicle (DMSO), 100 nM or 1000 nM of the PI3K/mTOR inhibitor AZ123 or the mTOR inhibitor Ku0063794 f...
ORGANISM(S): Homo sapiens (Human) 
2013-04-09 | PXD000185 | Pride
Tio2 phosphoenrichment Label free quantification Aml primary cells Pi3k/mtor inhibitors 
ProteomicsA protein phosphatase network controls the temporal and spatial dynamics of differentiation commitment in human epidermis
Epidermal homeostasis depends on a balance between stem cell renewal and terminal differentiation. The transition between the two cell states,termed commitment, is poorly understood. Here we characterise commitment by integrating transcriptomic and proteomic data from disaggregated primary human ker...
ORGANISM(S): Homo sapiens (Human) 
2017-10-18 | PXD003281 | Pride
Human Timecourse Keratinocytes Pkc U2os 
ProteomicsBIN2 and RAPTOR1B mutants phosphoproteomic analysis
Finely ground leaf tissue was used. Extraction was done in 8M urea, precipitated with acetone and methanol. Filter-assisted sample preparation (FASP) columns were used to further purify proteins. Purified proteins were reduced with TCEP, alkylated with iodoacetamide and digested into peptides with ...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2020-11-17 | MSV000086460 | MassIVE
ProteomicsStreptomyces coelicolor quantitative proteome and phosphoproteome analyses reveal proteins and phosphoproteins modulating differentiation and secondary metabolism
Investigation of proteome and phosphoproteome changes during Streptomyces coelictor life cycle using TMT tagging and calcium precipitation - titanium dioxide enrichment
ORGANISM(S): Streptomyces coelicolor 
2018-05-25 | PXD005558 | Pride
Streptomyces coelicolor Phosphoproteomics Tio2 
ProteomicsArabidopsis thaliana whole-proteome BIN2 kinase assay
Finely ground leaf tissue of wild-type columbia-0 ecotype was used. Protein extraction was performed in buffer-saturated phenol and high sucrose buffer. Extracted proteins were incubated with purified recombinant GST or GST-BIN2 kinase. Filter-assisted sample preparation (FASP) columns in prese...
ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702) 
2020-11-17 | MSV000086462 | MassIVE
ProteomicsProteome and phosphoproteome analysis in chronically MTOR-deficient clones
Shotgun-proteomics and phosphoproteomics analysis of clonal pancreatic tumor cell lines upon long-term ablation of the energy master kinase regualator mTOR
ORGANISM(S): Mus musculus (Mouse) 
2022-05-20 | PXD027779 | Pride
Murine cell lines Pancreatic cancer Lc-ms/ms 
ProteomicsMachine learning from large-scale proteomics accurately ranks anti-cancer drugs based on efficacy
We present an approach, named Drug Ranking Using ML (DRUML), which uses omics data to produce ordered lists of > 400 drugs based on their effectiveness in decreasing cancer cell proliferation. We trained and validated DRUML using in-house proteomics and phosphoproteomics data from a panel of 26 AML,...
ORGANISM(S): Homo sapiens (Human) 
2021-02-22 | PXD019591 | Pride
Cancer Proteomics Drug efficacy prediction Druml Machine learning Phosphoproteomics Drug ranking 
ProteomicsAn increase in oxidative stress downstream of mTORC1 but not AKT causes a proliferative defect in cancer cells with acquired resistance to PI3K/mTOR inhibitors
Proteomics and phosphoproteomics analysis of MCF7 cells sensitive and resistant to the PI3K inhibitor GDC-0941. The analysis was done for parental and 3 resistant cell lines maintained in the presence or the absence of the drug. One of the resistant cell lines (G2) was also treated with vehicle or t...
ORGANISM(S): Homo sapiens (Human) 
2018-04-09 | PXD003594 | Pride
Human Phosphoproteomics Metabolism Ltq-orbitrap Tio2 Pi3k resistance Q-exactive plus 
ProteomicsNeuroblastoma intratumor heterogeneity
Proteome and phosphoproteome analysis of 10 patient derived xenografts from one neuroblastoma tumor
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2022-02-28 | PXD007901 | Pride
Neuroblastoma Pdx 
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