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ProteomicsTMT-labeled mass spectrometry-based proteomics analysis in NCI-H1581 lung cancer cells upon AZD4547 treatment
FGFR1-amplified lung cancer cell line NCI-H1581 was treated with FGFR inhibitor AZD4547 (0.1μM) or DMSO vehicle for 24 hours. Then TMT-labeled mass spectrometry-based proteomics was carried out in cell lysates to analyze the differentially expressed proteins between two groups.
ORGANISM(S): Homo sapiens (Human) 
2022-10-13 | PXD032227 | Pride
Tmt-labeled mass spectrometry Azd4547 Nci-h1581 
Proteomics29-Plex Tandem Mass Tag Mass Spectrometry Enabling Accurate Quantification by Interference Correction
Tandem mass tag (TMT) mass spectrometry is a mainstream isobaric chemical labeling strategy for profiling proteomes. Here we present a 29-plex TMT method to combine the 11-plex and 18-plex labeling strategies. The 29-plex method was examined with a pooled sample composed of 1x, 3x and 10x E. coli pe...
ORGANISM(S): Homo sapiens (Human) 
2023-03-10 | PXD033865 | Pride
Mass spectrometry  liquid chromatography  proteomics  proteome  tandem mass tag  ratio compression  interference  data processing 
UnknownOptimized Workflow for Multiplexed Phosphorylation Analysis of TMT-Labeled Peptides Using High-Field Asymmetric Waveform Ion Mobility Spectrometry.
Not available
S-EPMC6996458 | biostudies-literature
ProteomicsProteome of the Developing Murine Lens Through Mass Spectrometry
The proteome is a term used for the entire catalog of proteins present in a cell at any time. In here, we investigated the proteome of developing mouse lens through mass spectrometry-based protein sequencing. We extracted mouse lenses at six developmental time points, which included two embryonic (E...
ORGANISM(S): Mus musculus (Mouse) 
2018-01-22 | PXD006381 | Pride
Mouse ocular lens Mass-spectrometry Proteome 
ProteomicsExtensive Peptide Fractionation and y1 Ion-based Interference Detection Enable Accurate Quantification by Isobaric Labeling and Mass Spectrometry
Isobaric labeling quantification by mass spectrometry (MS) has emerged as a powerful technology for multiplexed large-scale protein profiling, but measurement accuracy in complex mixtures is confounded by the interference from co-isolated ions, resulting in ratio compression. Here we report that the...
ORGANISM(S): Escherichia coli 
2022-02-28 | PXD005797 | Pride
Isobaric labeling Proteomics Tmt Mass spectrometry And ratio compression Itraq Lc-ms/ms 
ProteomicsOptimized TMT-based quantitative cross-linking mass spectrometry strategy for large-scale interactomic studies
Cross-linking mass spectrometry is a powerful method for the investigation of protein-protein interactions from highly complex samples. XL-MS combined with tandem mass tag labeling holds the promise of large-scale PPI quantification. However, a robust and efficient TMT-based XL-MS quantification met...
ORGANISM(S): Homo sapiens (Human) Escherichia coli Bacteria 
2022-04-07 | PXD031114 | Pride
Faims Hek Ratio distortion E. coli Mass spectrometry Lumos Dsso Xl-ms Interactome Stepped hcd Cross-linking Interference Sps 
Unknown29-Plex tandem mass tag mass spectrometry enabling accurate quantification by interference correction.
Not available
S-EPMC9588555 | biostudies-literature
ProteomicsChicken Tandem-Mass-Tag quantitative mass spectrometry analysis
About “MS210144-1 to MS210144-12”: 6 samples: cell body-1#, cell body-2#, cell body-3#, Migrasome-1#, Migrasome-2#, Migrasome-3#, were labeled with tandem mass tags (TMT) reagents and then mixed. These combined TMT-labeled peptides were separated into 12 fractions (shown in list: from MS210144-1 to ...
ORGANISM(S): Gallus gallus (Chicken) 
2022-10-12 | PXD036647 | Pride
Tandem mass tags 
ProteomicsProteomeTools Part IV - TMT labeled non-modified tryptic peptides
The ProteomeTools project aims to derive molecular and digital tools from the human proteome to facilitate biomedical and life science research. Here, we describe the the generation and multimodal LC-MS/MS analysis of >500,000 tryptic synthetic peptides labeled with tandem mass tags (TMT6plex).
ORGANISM(S): Homo sapiens (Human) 
2021-01-11 | PXD023119 | Pride
Synthetic peptide Tmt Proteometools Tandem mass tag 
ProteomicsMass spectrometry–based proteomic profiling of pleural tissue
This dataset contains mass spectrometry-based proteomic data generated from pleural tissuesamples.Proteins were extracted, digested, and labeled using TMT isobaric labeling for quantitative analysis. Samples were analyzed using LC–MS/MS to profile the global proteome.The dataset includes raw mass sp...
ORGANISM(S): Homo sapiens (Human) 
2026-06-29 | PXD073793 | Pride
Mass spectrometry  lc–ms/ms  pleural tissue 
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