Mapping the subcellular proteome using a differential fractionation and an orthogonal nycodenz fractionation combined with quantitative mass spectrometry using isobaric labeling (TMT) in MS2
Comprehensive mass spectrometry (MS)-based proteomics is now feasible, but reproducible and multiplexed quantification remains challenging especially for analysis of post-translational modifications (PTMs), such as phosphorylation. Here we compared the most popular quantification techniques for phos...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast)
Cross-linking mass spectrometry is a powerful method for the investigation of protein-protein interactions from highly complex samples. XL-MS combined with tandem mass tag labeling holds the promise of large-scale PPI quantification. However, a robust and efficient TMT-based XL-MS quantification met...
ORGANISM(S): Homo sapiens (Human) Escherichia coli Bacteria
Quantitative analysis of proteomes across multiple time points, organelles, and perturbations is essential for understanding both fundamental biology and disease states. The development of isobaric tags (e.g. TMT) have enabled the simultaneous measurement of peptide abundances across several differe...
Differential-Nycodenz fractionation analyzed with an orthogonal fractionation of two combined differential fractions (Heavy and Light mitochondrial (ML)) using rate zonal centrifugation. Analysis is performed using isobaric labeling in TMT-MS2
