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ProteomicsTMTpro-18plex: The Expanded and Complete Set of TMTpro Reagents for Sample Multiplexing
The development of the TMTpro-16plex series expanded the breadth of commercial isobaric tagging reagents by nearly 50% over classic TMT-11plex. In addition to the described 16plex reagents, the proline-based TMTpro molecule can accommodate two additional combinations of heavy carbon and nitrogen iso...
ORGANISM(S): Homo sapiens (Human) 
2021-04-28 | PXD024275 | Pride
Real-time search Eclipse Byl-719 Pten Kin-193 18plex Tmtpro Mcf10a 
UnknownTMTpro-18plex: The Expanded and Complete Set of TMTpro Reagents for Sample Multiplexing.
Not available
S-EPMC8210943 | biostudies-literature
ProteomicsWhole cell proteomics of iNeurons and iDA neurons at day 23 from Control, ASAH1-/- and SMPD1-/-
This experiment describes a quantitative proteomic analysis of whole-cell lysates from induced neurons (iN) and induced dopaminergic neurons (iDA) differentiated from human embryonic stem cells of three genotypes: control, ASAH1 knockout, and SMPD1 knockout. Triplicate biological samples were prepar...
ORGANISM(S): Homo sapiens (Human) 
2025-08-12 | PXD067201 | Pride
Pd In Ida Lysosome dysfunction Neurodegeneration Tmtpro 
ProteomicsTMTpro-18plex analysis of HEK293T cells transfected with DCAF siRNAs
HEK293T cells transfected with control, CUL4, COP1, DCAF3, DCAF14, or RBBP7 siRNA were lysed in 150 µL guanidine buffer. After heating and sonication, the lysates were centrifuged at 20,000 × g for 15 min at 4°C. The supernatants were recovered, and proteins (150 µg each) were purified by methanol-c...
ORGANISM(S): Homo Sapiens (human) 
2025-11-14 | PXD057876 | JPOST Repository
ProteomicsQuantitative Global and Phospho-proteomics analysis of rapamycin-treated human SU-DHL-4 cells by LC-MSMS
Global protein profiling and global phospho-protein profiling in human diffuse large B cell lymphoma cell line SU-DHL-4 treated with Rapamycin at two time points compared with DMSO control was performed by Poochon Scientific using the TMT-18plex labeling based quantitative proteomics method and liqu...
ORGANISM(S): Homo sapiens (Human) 
2025-09-03 | PXD067812 | Pride
Lc-msms Global protein profiling Rapamycin Su-dhl-4 Tmt multiplex Global phospho-protein profiling Dlbcl 
ProteomicsTMTpro-18plex analysis of MM1.S cells treated with PROTACs
MM1.S cells were treated with DMSO, 0.3 µM Po-P, 0.3 µM Le-P, 0.3 µM F-P, 0.3 µM Cl-P, or 0.3 µM F3C-P for 16 h in three biological replicates. The cells were lysed in 100 µL guanidine buffer (6 M guanidine-HCl, 100 mM HEPES-NaOH, pH 7.5, 10 mM TCEP, 40 mM chloroacetamide). After heating and sonicat...
ORGANISM(S): Homo Sapiens (human) 
2023-07-22 | PXD037178 | JPOST Repository
ProteomicsTMTpro-18plex analysis of NTERA-2 cells treated with PROTACs
NTERA-2 cells were treated with DMSO, 0.3 µM Po-P, 0.3 µM Le-P, 0.3 µM F-P, 0.3 µM Cl-P, or 0.3 µM F3C-P for 16 h in three biological replicates. The cells were lysed in 100 µL guanidine buffer (6 M guanidine-HCl, 100 mM HEPES-NaOH, pH 7.5, 10 mM TCEP, 40 mM chloroacetamide). After heating and sonic...
ORGANISM(S): Homo Sapiens (human) 
2023-07-22 | PXD037179 | JPOST Repository
ProteomicsTMTpro-18plex analysis of siSTK38 or siLuc HeLa cells treated with EBSS or LLOMe
HeLa cells were transfected with STK38 siRNA or Luc siRNA and then treated with EBSS or LLOMe in three biological replicates. The cells were lysed in 500 µL guanidine buffer (6 M guanidine-HCl, 100 mM HEPES-NaOH, pH 7.5, 10 mM TCEP, 40 mM chloroacetamide). After heating and sonication, proteins (330...
ORGANISM(S): Homo Sapiens (human) 
2023-11-21 | PXD039312 | JPOST Repository
ProteomicsTMTpro-18plex analysis of MM1.S cells treated with thalidomide and its derivatives
MM1.S cells were treated with DMSO or 10 µM thalidomide or its derivatives for 5 h in two biological replicates. The cells were lysed in 200 µL guanidine buffer (6 M guanidine-HCl, 100 mM HEPES-NaOH, pH 7.5, 10 mM TCEP, 40 mM chloroacetamide). After heating and sonication, proteins (100 µg each) wer...
ORGANISM(S): Homo Sapiens (human) 
2023-07-22 | PXD041812 | JPOST Repository
ProteomicsA programmed decline in ribosome levels governs human early neurodevelopment
How reductions in ribosome biogenesis can result in tissue- and developmental-specific defects remain a mystery. Here we describe allelic variants in the ribosome biogenesis factor AIRIM/C1orf109 that are primarily associated with neurodevelopmental disorders. Using human cerebral organoids in combi...
ORGANISM(S): Homo sapiens (Human) 
2025-04-30 | PXD063298 | Pride
Tmtpro 18plex Ribosome Mtor 
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