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ProteomicsMass spectrometry of co-immunoprecipitation using tobacco Maturase K as bait
Chloroplast group IIA introns derive from bacterial ribozymes. Their splicing likely requires Maturase K (MatK), which has been largely inaccessible to functional analyses being itself a chloroplast intron-encoded protein. Here we used co-immunoprecipitation coupled with proteomics to identify prote...
ORGANISM(S): Nicotiana tabacum (Common tobacco) 
2026-02-10 | PXD060055 | Pride
Lc-ms/ms Tobacco Co-immunoprecipitation Seedilings Chloroplasts 
ProteomicsSize-exclusion chromatography of soluble chloroplast proteins from Nicotiana tabacum (tobacco)
Maturase K (MatK) is the only splicing factor encoded in the chloroplast genomes of land plants. Here we used a transplastomic Nicotiana tabacum line, in which the plastidial MatK gene is C-terminally tagged with a 3x-HA tag, to conduct size-exclusion chromatography of soluble protein extracts enric...
ORGANISM(S): Nicotiana tabacum (Common tobacco) 
2026-02-10 | PXD069946 | Pride
Maturase k Lc-ms/ms Tobacco Dia Size-exclusion chromatography Chloroplast 
ProteomicsMass spectrometry of co-immunoprecipitation using Arabidopsis MKIP1-YFP as bait
Arabidopsis thaliana (Arabidopsis) MATURASE K INTERACTING PROTEIN1 (MKIP1) is a plastid-localized, non-canonical member of the starch-branching enzyme family. Here we used co-immunoprecipitation (IP) coupled with proteomics to identify proteins bound to MKIP1 in stably transformed Arabidopsis lines ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-02-10 | PXD060108 | Pride
Arabidopsis Maturase k Lc-ms/ms Co-immunoprecipitation Mkip1 
UnknownFood addiction, executive function and mood in adolescents with obesity seeking treatment.
Not available
S-EPMC8086816 | biostudies-literature
ProteomicsLabel-free shotgun proteomics of S. cerevisiae producing starch-like glucans
The synthesis of starch-like glucans can be reconstituted in S. cerevisiae using the genes from Arabidopsis thaliana: heterologous expression of SS1 to SS4, BE2, BE3, ISA1, and ISA2, together with a bacterial ADPglucose pyrophosphorylase gene for substrate provision in yeast strain 29, yielded insol...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-09-05 | PXD032241 | Pride
Lc-ms/ms Progenesisqi Yeast 
UnknownThe Biological and Anthropogenic Soundscape of an Urbanized Port - the Charleston Harbor Estuary, South Carolina, USA.
Not available
S-EPMC10115300 | biostudies-literature
TranscriptomicsRIP-seq to identify transcripts interacting with pluripotency factors in mouse embryonic stem cells
Sox2 is a master transcriptional regulator of embryonic development. Having found that Sox2 interacts with RNA-binding proteins, we designed an experiment to discover RNAs associated with Sox2 and other pluripotency factors. Briefly, we used RNA immunoprecipitation followed by high-throughput sequen...
ORGANISM(S): Mus musculus 
2019-02-11 | E-MTAB-7640 | biostudies-arrayexpress
TranscriptomicsThe effect of progressive weight loss on metabolic function
The purpose of this study was to evaluate the effect of progressive weight loss (5, 10, 15% weight loss) on metabolic function such as multi-organ insulin sensitivity and beta-cell function in obese people. We conducted microarray analysis to determine the effect of progressive weight loss on adipos...
ORGANISM(S): Homo sapiens 
2016-02-26 | E-GEOD-70529 | biostudies-arrayexpress
ProteomicsProteomics of Arabidopsis thaliana leaves after silencing of MKIP1 or uL4c
Arabidopsis thaliana (Arabidopsis) MATURASE K INTERACTING PROTEIN1 (MKIP1) is a plastid-localized, non-canonical member of the starch-branching enzyme family. Inducible silencing of MKIP1 by estradiol-treatment results in pale newly emerged leaves. Here we analysed the proteome of these leaves from ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-02-10 | PXD067382 | Pride
Arabidopsis Lc-ms/ms Dia Ribosome Chloroplast 
UnknownStatus and subjective well-being: A conceptual replication and extension of Anderson et al. (2012).
Not available
S-EPMC11410214 | biostudies-literature
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