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ProteomicsIdentification of binding partners of the purified TOM complex from HEK293T cells
To investigate the interactome of the human translocase of the outer mitochondrial membrane (TOM) complex, we performed immunoaffinity purification of TOMM22-FLAG from HEK293T cells followed by LC-MS/MS analysis. This approach allowed us to identify proteins that co-purify with the TOM complex under...
ORGANISM(S): Homo sapiens (Human) 
2026-03-17 | PXD064520 | Pride
Tom complex Tomm22-flag ip Hek293t 
ProteomicsPINK1-TOM-TIM23 complex SDA XL-MS
3xFLAG-tagged human wild-type PINK1 was expressed and immunoprecipitated with anti-FLAG beads. Chemical cross-linking MS (XL-MS) analysis using SDA and UV exposure was performed followed by in solution trypsin digestion and MS analysis.
ORGANISM(S): Homo Sapiens (human) 
2025-05-29 | PXD058526 | JPOST Repository
ProteomicsTOM core complex - Cryo-EM Structure of the TOM Core Complex from Neurospora crassa
The structure of the TOM-complex from Neurospora crassa was characterized using single particle electron microscopy. In order to unambigously identify and characterize the constituting subunits and exclude the presence of previously uncharacterized subunits, the samples were analyzed using proteolyt...
ORGANISM(S): Neurospora crassa 
2017-08-16 | PXD006827 | Pride
Tom Neurospora crassa 
TranscriptomicsMicroarray-assisted Cloning of Mutants: Expression Profiling of tom-1 and unc-43
Analysis of gene expression data in two C.elegans mutant strains: KP3293 tom-1(nu468) and KP3365 unc-43(n1186); hif-1(nu469). These results support the utility of microarray hybridizations to facilitate positional cloning.
ORGANISM(S): Caenorhabditis elegans 
2007-06-27 | E-GEOD-2210 | biostudies-arrayexpress
ProteomicsTranslocase of the Outer Membrane (TOM complex) of hydrogenosomes in Trichomonas vaginalis
Mitochondria originated from α-proteobacterial endosymbionts, and the endosymbiont-to-organelle transition is tightly linked to the establishment of protein import pathways. The initial import of most proteins is mediated by the translocase of the outer membrane (TOM). Although TOM is common to all ...
ORGANISM(S): Trichomonas vaginalis G3 
2019-01-07 | PXD010850 | Pride
Mitochondria Tom complex Trichomonas vaginalis Protein import/translocation Hydrogenosomes 
ProteomicsA large-scale map of transient and constitutive interactors of human TOM complex enabled by crosslinking
The translocase of the outer mitochondrial membrane (TOM) is the major entry gate for the over 1,000 proteins that are continuously being imported into the organelle. While the core TOM complex is evolutionarily preserved between yeast and human, the human mitochondrial proteome is significantly lar...
ORGANISM(S): Homo sapiens (Human) 
2026-03-17 | PXD065392 | Pride
Bottom-up ms Cross-linking ms Affinity purification ms Human mitochondria Tom complex 
ProteomicsMapping protein interactions in the active TOM-TIM23 supercomplex
Nuclear-encoded mitochondrial proteins destined for the matrix have to be transported across two membranes. The TOM and TIM23 complexes facilitate the transport of precursor proteins with N-terminal targeting signals into the matrix. During transport, precursors are recognized by the TIM23 complex i...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-12-13 | PXD028002 | Pride
Lc-msms Tim Mitochondria Translocation Structural modelling Tom23 Saccharomyces cerevisiae Crosslinking 
ProteomicsTom20 gates PINK1 activity and mediates its tethering of the TOM and TIM23 translocases upon mitochondrial stress
Mutations in PTEN-induced putative kinase 1 (PINK1) cause autosomal recessive early onset Parkinson disease (PD). PINK1 is a Ser/Thr kinase that regulates mitochondrial quality control by triggering mitophagy mediated by the ubiquitin ligase Parkin. Upon mitochondrial damage, PINK1 accumulates on th...
ORGANISM(S): Homo sapiens (Human) 
2024-03-02 | PXD047765 | Pride
Pink1 Human Tom complex Mitochondria Tim complex Parkinson's 
UnknownAtomic structure of human TOM core complex.
Not available
S-EPMC7522991 | biostudies-literature
ProteomicsTranslocase of the Outer Membrane (TOM complex) of hydrogenosomes in Trichomonas vaginalis
Mitochondria originated from α-proteobacterial endosymbionts, and the endosymbiont-to-organelle transition is tightly linked to the establishment of protein import pathways. The initial import of most proteins is mediated by the translocase of the outer membrane (TOM). Although TOM is common to all ...
ORGANISM(S): Trichomonas vaginalis 
2022-03-01 | PXD009474 | Pride
Mitochondria Tom complex Trichomonas vaginalis Protein import/translocation Hydrogenosomes 
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