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ProteomicsFollicular cells Top-down proteomic
In mammals, the capacity of the female germ cell, the oocyte, to develop into embryo is acquired throughout meiotic maturation. Immature oocyte cannot be fertilized while mature oocyte is apt to accept spermatozoa and to develop an embryo. In a follicle, the oocyte is surrounded by mural granulosa c...
ORGANISM(S): Bos taurus (Bovine) 
2017-04-10 | PXD004892 | Pride
Oocytes Μlc-ms/ms Cumulus cells Top-down proteomic Granulosa cells 
ProteomicsStradard protein and yeast top-down data
We developed a novel dissociation strategy in the data acquisition process, the Mesh strategy to dissociate multiple charge states of one proteoform with stepped HCD (high collision energy dissociation). We proved that the ‘Mesh’ strategy could generate fragments with improved sequence coverage and...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-06-09 | PXD022941 | Pride
Stepped hcd Instrument control Real-time Mesh Top-down 
ProteomicsQuantifying Positional Isomers (QPI) by top-down mass spectrometry
Proteomics has exposed a plethora of post-translational modifications, but demonstrating functional relevance requires new approaches. Top-down proteomics can characterize co-occurring modifications in terms of localization, abundance and hierarchy. Here, we present a top-down MS analysis workflow f...
ORGANISM(S): Homo sapiens (Human) 
2021-03-18 | PXD012084 | Pride
Top-down mass spectrometry Positional isomers Quantitation Phosphorylation 
ProteomicsPredicting electrophoretic mobility of proteoforms for large-scale top-down proteomics
Large-scale top-down proteomics characterizes proteoforms in cells globally with high confidence and high throughput using reversed-phase liquid chromatography (RPLC)-tandem mass spectrometry (MS/MS) or capillary zone electrophoresis (CZE)-MS/MS. The false discovery rate (FDR) from the target-decoy ...
ORGANISM(S): Escherichia coli 
2020-02-17 | PXD017265 | Pride
Top-down proteomics Electrophoretic mobility Cze-ms Proteoform 
ProteomicsSingle-shot top-down proteomics with capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry for identification of nearly 600 Escherichia coli proteoforms
Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-MS/MS) has been recognized as an invaluable platform for top-down proteomics. However, the scale of top-down proteomics from CZE-MS/MS is still limited due to the low loading capacity and narrow separation windo...
ORGANISM(S): Escherichia coli 
2017-10-30 | PXD007273 | Pride
Top-down proteomics Cze-ms/ms E.coli 
ProteomicsBottom-up and top-down LC-MS/MS of ribosomal purifications
Ribosomal purifications of spinach 70S ribosome and human 40S and 60S ribosomal subunits were analyzed with bottom-up LC-MS/MS to identify proteins comprising purified complexes as well as co-purified proteins. In order to assess and quantify proteoforms of ribosomal proteins in ribosomal purificati...
ORGANISM(S): Spinacia oleracea Homo sapiens (Human) Escherichia coli 
2018-06-26 | PXD008881 | Pride
Ribosome Top-down Bottom-up 
ProteomicsCZE-MS/MS-based quantitative top-down proteomics reveals significant proteoform differences between healthy and Alzheimer's disease human brains
Alzheimer’s disease (AD) is a neurodegenerative disorder characterized by cognitive decline and pathological protein aggregation. Comprehensive protein characterization of human brains of AD patients and comparison with healthy samples is critical for pursuing a better understanding of the molecular...
ORGANISM(S): Homo sapiens (Human) 
2025-11-21 | PXD065459 | Pride
Top-down proteomics Human brain Proteoform Tau phosphorylation. Cze-ms/ms Alzheimer's disease 
ProteomicsLaser capture microdissection-capillary zone electrophoresis-tandem mass spectrometry (LCM-CZE-MS/MS) for spatially resolved top-down proteomics: a pilot study of zebrafish brain
Mass spectrometry (MS)-based spatially resolved top-down proteomics (TDP) of tissues is crucial for understanding the roles played by microenvironmental heterogeneity in the biological functions of organs and for discovering new proteoform biomarkers of diseases. There are few published spatially re...
ORGANISM(S): zebrafish metagenome 
2023-03-11 | PXD027741 | Pride
Spatially resolved top-down proteomics  capillary zone electrophoresis-tandem mass spectrometry  laser capture microdissection  zebrafish brain  proteoform  high sensitivity  neuropeptide hormone activity  motor activity 
ProteomicsCapillary zone electrophoresis-tandem mass spectrometry for top-down proteomics of mouse brain integral membrane proteins
Mass spectrometry (MS)-based top-down characterization of integral membrane proteins (IMPs) is crucial for understanding their functions in biological processes. However, it is technically challenging due to their low solubility in typical MS-compatible buffers. In this work, for the first time, we ...
ORGANISM(S): Mus musculus (Mouse) 
2023-12-07 | PXD042298 | Pride
Top-down proteomics  integral membrane protein  proteoform  capillary zone electrophoresis-mass spectrometry  mouse brain 
ProteomicsBottom-up LC-MS/MS and top-down LC-MS analysis of B-phycoerythrin
Analysis of fluorescent assembly B-phycoerythrin with a combination of bottom-up and top-down mass spectrometry to reveal heterogeneity of proteoforms and characterize their chromophorylations.
ORGANISM(S): Porphyridium purpureum 
2019-05-09 | PXD011275 | Pride
Bpe Phycoerythrin Proteoform Top-down Bilin 
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