Sort   by:  
 Page size 
ProteomicsFollicular cells Top-down proteomic
In mammals, the capacity of the female germ cell, the oocyte, to develop into embryo is acquired throughout meiotic maturation. Immature oocyte cannot be fertilized while mature oocyte is apt to accept spermatozoa and to develop an embryo. In a follicle, the oocyte is surrounded by mural granulosa c...
ORGANISM(S): Bos taurus (Bovine) 
2017-04-10 | PXD004892 | Pride
Oocytes Μlc-ms/ms Cumulus cells Top-down proteomic Granulosa cells 
MetabolomicsProteomic and metabolomic profiling of extracellular vesicles produced by human gut archaea

Gastrointestinal bacteria interact with the host and each other through various mechanisms, including the production of extracellular vesicles (EVs). However, the composition and potential roles of EVs released by gut archaea are poorly understood. Here, we study EVs produced by four strains of h...

2025-07-01 | MTBLS12422 | MetaboLights
ProteomicsCharacterization of intact proteomic profile of senescent-associated secretory phenotype (SASP) by top-down mass spectrometry.
Senescent cells are increasingly recognized as major contributors to age-related diseases, primarily through the secretion of bioactive molecules known as the senescence-associated secretory phenotype (SASP). The SASP plays a crucial role in promoting various age-related conditions, including neurod...
ORGANISM(S): Homo sapiens (Human) 
2025-12-29 | PXD061829 | Pride
Senescence  aging  top-down proteomics  proteoforms  biomarkers  sasp  senescence-associated secretory phenotype 
ProteomicsSulfolobus islandicus top-down LC-MSMS
Protein posttranslational methylation and acetylations have been reported to occur in archaea, including members of the genus Sulfolobus, but have not been characterized on a proteome-wide scale. Sulfolobus chromatin proteins are known to be methylated and acetylated on lysine side chains, resemblin...
ORGANISM(S): Sulfolobus islandicus LAL14/1 
2016-09-13 | PXD003074 | Pride
Archaea Top-down Lc-msms Orbitrap fusion 
ProteomicsTop-Down proteomics and Bottom-Up terminomics for the Detection of Protein Termini in C. elegans
Here we investigate how top-down proteomics (TDP) can be of value for the detection of protein termini. Either GELFrEE or solid-phase enrichment was conducted for pre-fraction. To verify the detected N-termini HUNTER (High-efficiency Undecanal-based N Termini EnRichment) was employed. Reductive dime...
ORGANISM(S): Caenorhabditis elegans 
2022-09-26 | PXD033668 | Pride
Orbitrap q exactive plus Gelfree Mspathfinder Top-down proteomic C. elegans Orbitrap fusion lumos Informed proteomics Bottom-up proteomics Prosight 
ProteomicsSpatially-Resolved Top-Down Proteomics of Zebrafish Brain Using Capillary Zone Electrophoresis-Tandem Mass Spectrometry
Understanding region-specific proteoform profiles in the brain is crucial for deciphering neural function and identifying potential therapeutic targets. Here, we present a comprehensive label-free quantitative top-down proteomics (TDP) analysis of distinct zebrafish brain regions using capillary zon...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2026-06-08 | PXD070925 | Pride
Proteoform Top-down proteomics Zebrafish brainregions 
ProteomicsOn-capillary cell lysis enables top-down proteomic analysis of single HeLa cells by CE-MS/MS
Proteomic analysis of limited samples and single cells requires specialized methods that prioritize high sensitivity and minimize sample loss. Consequently, sample preparation is one of the most important steps in limited sample analysis workflows to prevent sample loss. In this work, we have elimin...
ORGANISM(S): Homo sapiens (Human) 
2022-10-26 | PXD035339 | Pride
Top-down proteomics Single-cell top-down proteomics Tandem mass spectrometry Single-cell proteomics Capillary electrophoresis Ce-ms/ms 
ProteomicsTop Down Proteomic analysis of Gingival Crevicular Fluid in deciduous, exfoliating and permanent teeth in children
Gingival Crevicular Fluid, a plasma-derived exudate present in the gingival crevice was collected from deciduous, exfoliating and permanent teeth from 20 children (60 samples) with the aim to characterize and quantify by a mass spectrometry based top-down proteomic approach, the peptide/proteins in ...
ORGANISM(S): Homo sapiens (Human) 
2020-07-09 | PXD016010 | Pride
Top down Proteomics 
ProteomicsFunctionally Integrated Top-down Proteomics for Standardized Assessment of Human Induced Pluripotent Stem Cell-derived Engineered Cardiac Tissues
3-dimensional (3D) human induced pluripotent stem cell-derived engineered cardiac tissues (hiPSC-ECTs) have emerged as a promising alternative to 2-dimensional hiPSC-cardiomyocyte monolayer systems because hiPSC-ECTs are a closer representation of endogenous cardiac tissues and more faithfully refle...
ORGANISM(S): Homo sapiens (Human) 
2021-01-07 | PXD022814 | Pride
Top-down proteomics Human pluripotent stem cell Proteoform Engineered cardiac tissue Cardiac function 
ProteomicsHuman Salivary Cystatins - SALIVARY CYSTATINS: EXPLORING NEW POST-TRANSLATIONAL MODIFICATIONS AND POLYMORPHISMS BY TOP-DOWN HIGH RESOLUTION MASS SPECTROMETRY
In the present study various proteoforms of cystatin A, cystatin B, cystatin D, cystatin S, cystatin SN and cystatin SA were detected and characterized by a top-down HPLC-ESI-MS platform integrated by bottom-up strategies in the acidic soluble fraction of human saliva. The several proteoforms derive...
ORGANISM(S): Homo sapiens (Human) 
2017-10-13 | PXD007170 | Pride
S-type cystatins Post-translational modifications Cystatin a Top-down high-resolution mass spectrometry. Cystatin d Cystatin b Human saliva 
Sort   by:  
 Page size