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ProteomicsThiol reactive tandem mass tags for quantitative top-down proteomics
Here, we describe the development of a labeling strategy for TDP targeting thiol groups of cysteine residues with iodoTMTsixplex. While this method inherently excludes the quantification of cysteine-free proteoforms, it provides the opportunity of sixplexing and the implementation of multidimensiona...
ORGANISM(S): Escherichia coli Saccharomyces cerevisiae (Baker's yeast) 
2021-08-06 | PXD026310 | Pride
Isobaric labeling Gelfree High ph fractionation Yeast Iodotmtsixplex Quantitative top-down proteomics Saccharomyces cerevisae Escherichia coli 
ProteomicsTop-down characterization of lunasin in a commercial soybean-derived product
The presence of lunasin was investigated in two soybean products, raw soy seeds and a commercial soybean beverage powder. Lunasin from the commercial soybean derivative was purified by reverse phase high pressure liquid chromatography and bystrong anion exchange solid phase extraction. Lunasin was ...
ORGANISM(S): Glycine max 
2016-05-24 | PXD003064 | Pride
Glycation Proteomics Soybean Top-down Advanced glycation end produts 
UnknownTop-down proteomics reveals novel protein forms expressed in Methanosarcina acetivorans.
Not available
S-EPMC2832193 | biostudies-literature
ProteomicsMonitoring mAb proteoforms in mouse plasma using an automated immunocapture combined with top-down and middle-down mass spectrometry
We propose here a workflow based on an automated immunocapture followed by top-down and middle-down LC-MS/MS approaches to characterize mAb proteoforms spiked in mouse plasma. We demonstrate the applicability of our workflow on a large concentration range using pembrolizumab as a model. We also comp...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2024-08-27 | PXD040896 | Pride
Immunocapture Monoclonal antibody Proteoforms Middle-down mass spectrometry Top-down mass spectrometry 
UnknownAn integrated top-down and bottom-up strategy for broadly characterizing protein isoforms and modifications.
Not available
S-EPMC2693490 | biostudies-literature
ProteomicsVenom proteomics of Indonesian king cobra
Complementary bottom-up MS/MS analyses contributed to complete a locus-resolved venom phenotype map for O. hannah, the world's longest venomous snake and a species of medical concern across its wide distribution range in forests from India through Southeast Asia. Its venom composition convincingly e...
ORGANISM(S): Ophiophagus hannah (King cobra) (Naja hannah) 
2015-08-20 | PXD001726 | Pride
Snake venomics  elapidae  king cobra  ophiophagus hannah  top-down proteomics  top-down venomics 
ProteomicsSalivary proteomics of sP and sNP rats
Sardinian alcohol-preferring (sP) and Sardinina alcohol-non preferring (sNP) rats have been selectively bred for opposite alcohol preference and consumption. The project proposed to identify salivary markers distinguishing the two rat lines possibly correlated to alcohol preference, by a proteomic a...
ORGANISM(S): Rattus norvegicus (Rat) 
2017-11-03 | PXD006997 | Pride
Saliva submandibular rat alcohol-preferring 
ProteomicsAn integrated strategy reveals complex glycosylation of erythropoi-etin using top-down and bottom-up mass spectrometry
The characterization of therapeutic glycoproteins is challenging due to the structural micro- and macro-heterogeneity of the protein glycosylation. This study presents an in-depth strategy for glycosylation analysis using first-generation erythropoietin (epoetin beta), including a developed top-down...
ORGANISM(S): Mus musculus (Mouse) 
2021-06-14 | PXD024835 | Pride
Erythropoietin Bottom-up Top-down Mass spectrometry Glycosylation Python scripts 
UnknownIntegrated workflow for characterizing intact phosphoproteins from complex mixtures.
Not available
S-EPMC4031921 | biostudies-literature
ProteomicsIdentification of muscle biomarkers in McArdle disease
Glycogen storage disease type V (GSDV, McArdle disease) is a rare genetic myopathy caused by deficiency of the muscle isoform of glycogen phosphorylase (PYGM). This results in a block in the use of muscle glycogen as an energetic substrate, with subsequent exercise intolerance. The GSDV pathophysiol...
ORGANISM(S): Homo sapiens (Human) 
2022-06-09 | PXD031605 | Pride
Human Rp-lc-ms/ms Muscle Proteomic Itraq 
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