Wild type or kinase dead TTBK1 kinase is overexpressed in HEK293 cells, IP and seperated on SDSPAGE gel. TTBK1 corresponding bands are cut and analyzed on shotgun proteomics.
TTBK1 and TTBK2 unique substrate identifications in mouse cortical neurons through global phosphoproteomics studies. shRNA for both TTBK1 or TTBK2 was used to treat mouse cortical neurons, to knockdown each kinase.
