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ProteomicsEffect of TOPBEffect of TOPBP1 on BLM phosphosites
Identification of BLM phosphosites. The experiment is composed by 2 parts: i) identification of BLM phosphosites with DDA method ii) quantification of phosphosites with a targeted method.
ORGANISM(S): Homo sapiens (Human) 
2021-11-25 | PXD026460 | Pride
Phosphosites 
ProteomicsCharacterization of MPro substrate specificity by HTPS
Characterization of MPro substrate specificity by HTPS using temporal resolution (5min, 30min, 4hrs, 24hrs)
ORGANISM(S): Homo sapiens (Human) 
2025-07-02 | PXD044733 | Pride
Degradomics Htps 
ProteomicsInvestigating proteome remodeling as a function of mammalian cell size
Investigating proteome remodeling as a function of mammalian cell size Experiment design: Condition1: RPE1 cells treated with palbociclib (2days) Condition 2: RPE1 cells treated with palbociclib (6days) Condition 3: RPE1 cells treated with palbociclib (6days) +Torin1 Condition 4: RPE1 cells treated...
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD034934 | Pride
Tmt  cell size 
ProteomicsTargeted analysis of endogenous purification of YAP1 in HEK293A cell lines
Targeted analysis of endogenous purification of YAP1 in HEK293A cell lines
ORGANISM(S): Homo sapiens (Human) 
2023-03-04 | PXD032310 | Pride
Ipms Prm 
ProteomicsMS Analysis In vivo treatment of YAP1 SH tagged with phosphatase inhibitors
Investigation of YAP1 interactome plasticity via APMS
ORGANISM(S): Homo sapiens (Human) 
2023-03-04 | PXD032218 | Pride
Phospho Yap1 Apms 
ProteomicsTargeted experiment to validate genetic deletion in KO cells
Validation of genetic deletion by targeted proteomics
ORGANISM(S): Homo sapiens (Human) 
2023-03-04 | PXD032220 | Pride
Ko cells Prm 
ProteomicsTargeted analysis of PTPN14, YAP1, LATS1 and GFP SH-tagged pulldown
Targeted analysis of YAP1, LATS1 and PTPN14 interactome
ORGANISM(S): Homo sapiens (Human) 
2023-03-04 | PXD032221 | Pride
Yap1 Lats1 Ptpn14 Apms 
ProteomicsAn Amphipathic Helix in Brl1 is Required for Nuclear Pore Complex Biogenesis in S. cerevisiae - Lysate intermixing text (DIA)
The project was aimed at assessing the dynamic protein exchange of the nuclear pore complex during the affinity purification procedure with Brl1 and Nup170 bait. In brief, equal fractions of an affinity tagged strain grown in unlabeled medium and a wild-type culture grown in metabolically labeled me...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-08-17 | PXD032034 | Pride
Dia 
ProteomicsAn Amphipathic Helix in Brl1 is Required for Nuclear Pore Complex Biogenesis in S. cerevisiae – targeted analysis of cell lysate (PRM)
The project was aimed at assessing the metabolic labeling of NUPs in the full lysate upon SILAC labeling of growing yeast cultures, 90 min post labeling. This experiment was performed to exclude that the systematic labeling differences that we observed in KARMA assays with Brl1 bait were present alr...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-08-17 | PXD032016 | Pride
Cell lysate Prm 
ProteomicsAn Amphipathic Helix in Brl1 is Required for Nuclear Pore Complex Biogenesis in S. cerevisiae - KARMA analysis of Brl1 (DIA)
This project was aimed at analyzing the binding of Brl1 to the nuclear pore complex during the assembly process using the Kinetic Analysis of incorporation Rates in Macromolecular Assemblies (KARMA, Onischenko et al. 2020) method. In brief, we affinity purified Brl1 at several time points following ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-08-17 | PXD032017 | Pride
Dia Karma 
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