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In this work, we propose a new high-throughput ultrafast method for large scale proteomics approaches by speeding the classic filter aided sample preparation protocol, FASP. The new US-FASP method matches the analytical minimalism roles as time, cost, sample requirement, reagent consumption, energy ...
ORGANISM(S): Mus musculus (Mouse) Escherichia coli 
2020-06-08 | PXD018360 | Pride
10 µm formalin-fixed paraffin-embedded tissue sections were deparaffinized with xylene; homogenization was performed in lysis-buffer (20 mM Tris-HCl, pH 8.8, 200 mM glycine, 200 mM DTT, 4% SDS)in an ultrasonic bath; samples were incubated under continuous agitation first at 99 °C for 30 min, then a...
ORGANISM(S): Homo sapiens (Human) 
2020-05-18 | PXD012317 | Pride
Untargeted approaches and thus biological interpretation of metabolomics results are still hampered by the reliable assignment of the global metabolome as well as classification and (putative) identification of metabolites. In this work we present an liquid chromatography-mass spectrometry (LC-MS)-b...
2020-02-09 | MTBLS1217 | MetaboLights

This project is based on non targeted metabolomics analysis of cassava (Manihot esculenta) root samples. LC-MS/MS is used to obtain metabolite characteristic peaks and perform peak extraction, alignment, and quantification. Differential metabolites are screened, with a focus on flavonoid and phen...

2026-05-06 | MTBLS14086 | MetaboLights
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