Acute respiratory distress syndrome (ARDS) is a severe critical condition with a high mortality that is currently in focus given that it is associated with mortality caused by coronavirus induced disease 2019 (COVID-19). Neutrophils play a key role in the lung injury characteristic of non-COVID-19 A...
FACS sorted single cell proteomes of glioblastoma derived neutrophils. Brain tissue was dissociated and individual neutrophils were FACS sorted into 384 well plates into master mix containing. The cells were later processed on the CellenOne and analysed on an Orbitrap Astral with FAIMS Pro. The data...
Mini-bulk (500 cell) analysis of low density CD10+, low density CD10-, normal density CD10+ and brain neutrophils from glioblastoma patients with matched normal density CD10+ neutrophils from controls.
Analysis of low density neutrophils and normal density neutrophils derived from the peripheral blood of GBM patients as well as matched normal density neutrophils from control patients.
Hypoxemia is a defining feature of acute respiratory distress syndrome (ARDS), an often-fatal complication of pulmonary or systemic inflammation, yet the resulting tissue hypoxia, and its impact on immune responses, is often neglected. Here we showed that ARDS patients were hypoxaemic and monocytope...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse)
This project aimed to define the proteome of inflammatory lung neutrophils and determine how his is regulated by exposure to in vivo hypoxia. An acute lung injury was induced using nebulised LPS. Following LPS administration mice were housed in either normal room air or in a hypoxic chamber set at a...
The long-term impact of systemic hypoxia resulting from Acute Respiratory Distress Syndrome (ARDS) on the function of short-lived innate immune cells is unclear. We show that patients 3-6 months after recovering from ARDS have persistently impaired circulating neutrophil effector functions and an in...
The long-term impact of systemic hypoxia resulting from Acute Respiratory Distress Syndrome (ARDS) on the function of short-lived innate immune cells is unclear. We show that patients 3-6 months after recovering from ARDS have persistently impaired circulating neutrophil effector functions and an in...
