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ProteomicsA simple and efficient approach for preparing cationic coating with tunable electroosmotic flow for capillary zone electrophoresis-mass spectrometry-based top-down proteomics
Capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) has become a valuable analytical technique in top-down proteomics (TDP) in recent years. CZE-MS/MS-based TDP typically employs separation capillaries with neutral coatings (i.e., linear polyacrylamide, LPA). However, issues related ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-10-15 | PXD051862 | Pride
Top-down proteomics  proteoform  capillary zone electrophoresis-mass spectrometry  covalent cationic coating  reproducibility  large proteoforms 
ProteomicsQuantitative top-down proteomics reveals significant differences in histone proteoforms between metastatic and nonmetastatic colorectal cancer cells
As one of the leading causes of cancer deaths worldwide, colorectal cancer (CRC) development is closely associated with the accumulation of both genetic and epigenetic alterations. Many efforts have been made to investigate the role of epigenetic modifications in CRC metastasis. In this work, we pre...
ORGANISM(S): Homo sapiens (Human) 
2025-11-21 | PXD060160 | Pride
Top-down proteomics Post-translational modifications Capillary electrophoresis-mass spectrometry Colorectal cancer metastasis Histone proteoforms 
ProteomicsCapillary Zone Electrophoresis-High Field Asymmetric Ion Mobility Spectrometry-Tandem Mass Spectrometry for Top-down Characterization of Histone Proteoforms
Characterization of histone proteoforms with various post-translational modifications (PTMs) is critical for a better understanding of functions of histone proteoforms in epigenetic control of gene expression. Mass spectrometry (MS)-based top-down proteomics (TDP) is a valuable approach for delineat...
ORGANISM(S): Bos taurus (Bovine) 
2023-12-07 | PXD041357 | Pride
Histone Top-down proteomics High-field asymmetric waveform ion mobility spectrometry Post-translational modification Capillary zone electrophoresis 
ProteomicsCapillary zone electrophoresis-tandem mass spectrometry for top-down proteomics of mouse brain integral membrane proteins
Mass spectrometry (MS)-based top-down characterization of integral membrane proteins (IMPs) is crucial for understanding their functions in biological processes. However, it is technically challenging due to their low solubility in typical MS-compatible buffers. In this work, for the first time, we ...
ORGANISM(S): Mus musculus (Mouse) 
2023-12-07 | PXD042298 | Pride
Top-down proteomics  integral membrane protein  proteoform  capillary zone electrophoresis-mass spectrometry  mouse brain 
ProteomicsCoupling SDS-PAGE to capillary zone electrophoresis-tandem mass spectrometry for high-resolution top-down proteomics analysis of intact histone proteoforms
Top-down mass spectrometry (TDMS) is a powerful tool to reveal the mechanism of epigenetic regulation by histones. However, the high similarity of histone variants and their highly dynamic post-translational modifications (PTMs) make them as on-going challenge analytes for separation with traditiona...
ORGANISM(S): Bos taurus (Bovine) 
2024-10-15 | PXD048061 | Pride
Sds-page Top-down proteomics Post-translational modifications Histone proteoform Cze-ms/ms 
ProteomicsPilot evaluation of long-term reproducibility of capillary zone electrophoresis-tandem mass spectrometry for top-down proteomics of a complex proteome sample
In this work, for the first time, we performed a pilot investigation of long-term reproducibility of CZE-MS for TDP of a complex sample (i.e., a yeast cell lysate) to achieve a better understanding of advantages, issues and potential solutions of CZE-MS for large-scale TDP.
ORGANISM(S): Candida albicans (Yeast) Saccharomyces cerevisiae 
2024-03-20 | PXD046651 | Pride
Yeast proteome Capillary zone electrophoresis-tandem ms (cze-ms/ms) Top-down proteomics (tdp) Capillary cleanup Long-term reproducibility 
ProteomicsA bioenergetic shift is required for spermatogonial differentiation
A bioenergetic balance between glycolysis and mitochondrial respiration is particularly important for stem cell fate specification. It however remains to be determined whether undifferentiated spermatogonia switch their preference of bioenergy production during differentiation. In this study, we fou...
ORGANISM(S): Mus musculus (Mouse) 
2021-09-09 | PXD019136 | Pride
Spermatogonial differentiation Oxphos Glycolysis 
ProteomicsLarge-scale Top-down Proteomics of the Arabidopsis thaliana Leaf and Chloroplast Proteomes
We present a large-scale top-down proteomics study of plant leaf and chloroplast proteins, achieving the identification of over 4700 unique proteoforms. Using capillary zone electrophoresis coupled with tandem mass spectrometry analysis of offline size-exclusion chromatography fractions, we identify...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-01-05 | PXD034368 | Pride
Arabidopsis thaliana Post-translational modification Proteoform Transit peptide Singlet oxygen Chloroplast 
ProteomicsNanoparticle-aided nanoreactor for large-scale proteomics of few mammalian cells
Large-scale bottom-up proteomics of few even single cells is crucial for a better understanding of the roles played by cell-to-cell heterogeneity in disease and development. Novel proteomic methodologies with extremely high sensitivity are required for few even single-cell proteomics. Sample process...
ORGANISM(S): Homo sapiens (Human) 
2022-07-11 | PXD027135 | Pride
Nanoreactor Single-cell proteomics Mass-limited sample Nanoproteomics Bottom-up proteomics Nanoparticle Few-cell proteomics 
ProteomicsCapillary Zone Electrophoresis-Electron-Capture Collision-Induced Dissociation on a Quadrupole Time-of-Flight Mass Spectrometer for Top-Down Characterization of Intact Proteins
Mass spectrometry (MS)-based denaturing top-down proteomics (dTDP) requires high-capacity separation and extensive gas-phase fragmentation of proteoforms. Herein, we coupled capillary zone electrophoresis (CZE) to electron-capture collision-induced dissociation (ECciD) on an Agilent 6545 XT quadrupo...
ORGANISM(S): Bos taurus (Bovine) Equus caballus (Horse) 
2023-01-05 | PXD030373 | Pride
Electron-capture collision-induced dissociation Top-down Capillary zone electrophoresis 
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