Sort   by:  
 Page size 
ProteomicsCross-linking of the Endolysosomal System Reveals Potential Flotillin Structures and Cargo
Lysosomes are well-established as the main cellular organelles for the degradation of macromolecules and emerging as regulatory centers of metabolism. They are of crucial importance for cellular homeostasis, which is exemplified by a plethora of disorders related to alterations in lysosomal function...
ORGANISM(S): Homo sapiens (Human) 
2022-10-22 | PXD030532 | Pride
Lysosomal interactome Cross-linking ms 
ProteomicsGOLPH3 and GOLPH3L maintain Golgi localization of LYSET and a functional mannose 6-phosphate transport pathway
Glycosylation plays an important role for modifications of lipids and sorting of proteins that is regulated by asymmetric intra-Golgi distribution and SPPL3-mediated cleavage of Golgi enzymes. We found that cells lacking the Golgi N-acetylglucosamine phosphotransferase (GNPT) retention factor LYSET/...
ORGANISM(S): Homo sapiens (Human) 
2024-11-27 | PXD052083 | Pride
Golgi apparatus  lysosomes  mannose 6-phophate tagging  glycosyltransferase secretion  intramembrane proteolysis  copi vesicles 
ProteomicsThe mitochondrial Hsp70 controls the assembly of the F1FO-ATP synthase
The mitochondrial F1FO-ATP synthase produces the bulk of cellular ATP. The soluble F1 domain contains the catalytic head that is linked via the central stalk and the peripheral stalk to the membrane embedded rotor of the Fo domain. The assembly of the F1 domain and its linkage to the peripheral stal...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-01-11 | PXD033024 | Pride
Mitochondria F1fo-atp synthase Pulldown Mthsp70 
ModelsSchilling2009 - ERK distributive
Schilling2009 - ERK distributive This model has been exported from PottersWheel on 2009-04-20 18:57:44.  The PottersWheel Model Definition file can be obtained from the curation tab. This model is described in the article: Theoretical and experimental analysis links ...
2024-09-02 | BIOMD0000000270 | BioModels
ProteomicsInvestigation of Oligodendrocyte Precursor Cell Differentiation by Quantitative Proteomics
Oligodendrocytes, the myelinating cells of the central nervous system, are essential for correct brain function. They originate from oligodendrocyte precursor cells (OPCs) through a differentiation process which is only incompletely understood and impaired in a variety of demyelinating diseases. Bet...
ORGANISM(S): Rattus norvegicus (Rat) 
2019-06-21 | PXD012544 | Pride
Quantification Tmt labeling Oligodendrocytes Mass spectrometry Oligodendrocyte precursor cells 
ProteomicsThe Human Disease Gene LYSET is Essential for Lysosomal Enzyme Transport and Viral Infection
Lysosomes are key degradative compartments of the cell. Transport to lysosomes ismediated by tagging soluble enzymes with mannose 6-phosphate (M6P) by GlcNAc-1-phosphotransferase whose deficiency leads to the severe lysosomal storage disorder mucolipidosisII (MLII). Several viruses require lysosomal...
ORGANISM(S): Mus musculus (Mouse) 
2022-09-15 | PXD029609 | Pride
Data independent acquisition (dia) Tmem 251 Parallel reaction monitoring (prm) Targeted ms Label free quantification Mouse embryonic fibroblasts 
ProteomicsRapiGest Precipitation Depends on Peptide Concentration
The mass spectrometry-compatible surfactant RapiGest promotes the enzymatic digestion of proteins by facilitating their unfolding while retaining enzymatic activity. RapiGest consists of a hydrophilic head and a hydrophobic tail, which can be separated by acid hydrolysis. This allows for removal of ...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) Gallus gallus (Chicken) 
2021-09-07 | PXD025982 | Pride
In-solution digestion Lc-ms Rapigest Detergent Precipitation 
ProteomicsTwo step enrichment facilitates background reduction for proteomic analysis of lysosomes
Lysosomes comprise the main degradative compartment of almost all mammalian cells and are involved in various cellular functions, most of which are catalyzed by the lysosomal proteome. Lysosomal proteins are low abundant, complicating their analysis by mass spectrometry-based proteomics. To increase...
ORGANISM(S): Homo sapiens (Human) 
2024-08-01 | PXD048696 | Pride
Lysosomes  lysosome enrichment  superparamagnetic iron oxide particles (spions)  tmem192 Mass spectrometry  proteomics  organelles Hek 293 
ProteomicsTargeted Quantification of the Lysosomal Proteome in Complex Samples
The lysosome, as the main degradative organelle of eukaryotic cells, is involved in numerous cellular processes. A defect in one of its proteins often results in lysosomal storage diseases (LSDs). For the study of lysosomal proteins, mass spectrometry (MS) has emerged as the method of choice. Lysoso...
ORGANISM(S): Mus musculus (Mouse) 
2021-03-08 | PXD023278 | Pride
Targeted proteomics Parallel reaction monitoring Label-free quantification Lysosomes Data-independent acquisition 
ProteomicsIdentification of Poly Ethylene Glycol (PEG) and PEG-based Detergents Using Peptide Search Engines
Polyethylene glycol (PEG) is one of the most common polymer contaminations in MS samples. At present, the detection of PEG and other polymers relies largely on manual inspection of raw data which is laborious and frequently difficult due to sample complexity and retention characteristics of polymer ...
ORGANISM(S): Rattus rattus (Black rat) 
2018-05-28 | PXD009376 | Pride
Automated Polymer Mass spectrometry Peptide search engine Peg Poly ethylene glycol 
Sort   by:  
 Page size