Sort   by:  
 Page size 
ProteomicsA systems immunology study comparing innate and adaptive immune responses in adults to COVID-19 mRNA (BNT162b2/mRNA-1273) and adenovirus vectored vaccines (ChAdOx1-S)
We longitudinally profiled plasma proteomes in 54 adults vaccinated with the BNT162b2 (Pfizer-BioNTech) or ChAdOx1-S (Oxford-AstraZeneca) vaccines. Blood was collected pre-vaccination (V0) and 1-7 days after the 1st doses (BNT162b2 or mRNA-1273, V1) to assess innate and early adaptive responses. We ...
ORGANISM(S): Homo sapiens (Human) 
2023-03-01 | PXD036608 | Pride
Sars-cov-2 Proteomics Plasma Vaccination Covid-19 vaccine 
ProteomicsProtein and peptide turnover measurements using pulsed SILAC-TMT approach
We evaluated the feasibility of a workflow combining dynamic SILAC experiments with tandem mass tag (TMT)-labeling of ten pulse time-points. Replicate analysis established that the same reproducibility of turnover rates can be obtained for peptides as for proteins facilitating proteoform resolved in...
ORGANISM(S): Homo sapiens (Human) 
2018-02-09 | PXD008579 | Pride
Degradation rates Protein turnover Proteoforms Posttranslational cleavage Synthesis rates Tmt Splice variants Pulsed silac Posttranslational modification 
ProteomicsLinking post-translational modifications and protein turnover by site-resolved protein turnover (SPOT) profiling
Proteome-wide measurements of protein turnover have largely ignored the impact of post-translational modifications (PTMs). To address this gap, we employ stable isotope labelling and mass spectrometry to measure the turnover of >120,000 peptidoforms including >33,000 phosphorylated, acetylated and u...
ORGANISM(S): Homo sapiens (Human) 
2021-11-10 | PXD023218 | Pride
Human Ptms Turnover Silac 
ProteomicsTMT labeling for the masses: A robust and cost-efficient, in-solution labeling strategy
Isobaric stable isotope labeling using, for example, tandem mass tags (TMTs) is increasingly being applied for large-scale proteomic studies. Experiments focusing on proteoform analysis in drug time course or perturbation studies or in large patient cohorts greatly benefit from the reproducible quan...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2020-01-14 | PXD012703 | Pride
Stable isotope labeling Tandem mass tags Nhs ester chemistry Labeling efficiency 
ProteomicsTyrosine-protein phophatase non-receptor type 2 (PTPN2) deficiency increases short-lived effector T-cell survival and augments autonomous proliferation of T-cells
Whole proteome and phosphoproteome analysis of T-cells isolated from PTPN2 wild-type and knock-out micestrongly linked amplified T cell numbers to increased proliferation mediated by transcriptional activation of the signal transducer and activator of transcription (STAT) protein family. Subsequent ...
ORGANISM(S): Mus musculus (Mouse) 
2020-07-29 | PXD013122 | Pride
T-cells Ms3 Tmt Ptpn2 Phosphoproteomics 
ProteomicsThe OTUD6B-LIN28B-MYC axis determines cell cycle progression in multiple myeloma
Deubiquitylases (DUBs) remove ubiquitin from proteins. In the context of cancer, their inhibition can induce the degradation of oncoproteins, that may otherwise be “undruggable”. Multiple myeloma (MM) is the second most common hematological malignancy with poor outcome and high sensitivity towards u...
ORGANISM(S): Homo sapiens (Human) 
2022-10-12 | PXD027480 | Pride
Bioid Human Flag-ip Orbitrap Otud6b 
ProteomicsThe IMiD receptor CRBN determines quality control of transmembrane proteins
The complex architecture of transmembrane proteins requires quality control (QC) for folding and membrane positioning, a prerequisite for cellular homoeostasis and intercellular communication. However, it has remained unclear whether transmembrane proteins-specific QC hubs exist. Here we specify Cer...
ORGANISM(S): Homo sapiens (Human) 
2021-02-18 | PXD018244 | Pride
Imids Chaperones Membrane proteins Lumos Human Multiple myeloma 
ProteomicsEvaluation of of kinase acitivity profiling using chemical proteomics
Evaluation of kinase binding to immobilized inhibitors upon kinase activation. Comparison of cheomproteomic binding changes to phosphoproteomic activation profiles.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-10-21 | MSV000080272 | MassIVE
ProteomicsData, reagents, assays and merits of proteomics for SARS-CoV-2 research and testing
As the SARS-CoV-2 pandemic continues to spread, thousands of scientists around the globe have changed research direction to understand better how the virus works and to find out how it may be tackled. To facilitate proteomic research on SARS-CoV-2, we presents deep-scale proteomes of common cell lin...
ORGANISM(S): Chlorocebus Sabaeus (ncbitaxon:60711) Chlorocebus Aethiops (ncbitaxon:9534) Homo Sapiens (ncbitaxon:9606) 
2020-07-17 | MSV000085774 | MassIVE
ProteomicsLinking post-translational modifications and protein turnover by site-resolved protein turnover (SPOT) profiling
Proteome-wide measurements of protein turnover have largely ignored the impact of post-translational modifications (PTMs). To address this gap, we employ stable isotope labelling and mass spectrometry to measure the turnover of >120,000 peptidoforms including >33,000 phosphorylated, acetylated, and ...
ORGANISM(S): Homo Sapiens 
2022-03-14 | PXD023234 | panorama
Sort   by:  
 Page size