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The recent development within high-throughput technologies for expression profiling has allowed for parallel analysis of transcriptomes and proteomes in biological systems such as comparative analysis of transcript and protein levels of tissue regulated genes. Until now, such studies of have only in...
ORGANISM(S): Sus scrofa 
Secreted proteases act on interstitial tissue secretomes released from multiple cell types. Thus, substrate proteins might be part of higher molecular complexes constituted by many proteins with diverse and potentially unknown cellular origin. In cell culture these might be reconstituted by mixing n...
ORGANISM(S): Mus musculus (Mouse) 
2015-05-11 | PXD001643 | Pride
Stable VPS35 knockdown HeLa cells were generated using lentiviral short hairpin RNA (shRNA) construct. Isobaric tags for relative and absolute quantitation (iTRAQ)-based mass spectrometry was used to measure the changes of nuclear protein abundance in VPS35-depleted HeLa cells.
ORGANISM(S): Homo sapiens (Human) 
2022-08-12 | PXD027166 | Pride
In this study, we used an iTRAQ-based quantitative proteomic approach to reveal the release of proteolytic fragments from the cell surface or the ECM by BMP-1. Thirty-seven extracellular proteins were found in significantly higher or lower amounts in the conditioned medium of HT1080 cells overexpres...
ORGANISM(S): Homo sapiens (Human) 
2014-09-29 | PXD000786 | Pride
the proteome of skin lesions from HHD patients was assembled and used for iTRAQ to analyze changes in skin lesions compared with unaffected individuals.
ORGANISM(S): Homo sapiens (Human) 
2018-12-10 | PXD006286 | Pride
We employed UPLC-MS/MS with iTRAQ 8-plex labeling to quantitatively analyze the supernatant produced by two Chinese hamster ovary (CHO) cell lines (CHO K1SV and CHO CAT-S). In each case, the supernatant from the host and three transfected clones were analyzed at days 5, 7, and 10 of culture. A total...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2016-07-20 | PXD003462 | Pride
AGS cells were treated with metformin then performed iTRAQ proteomics techniques.
ORGANISM(S): Homo sapiens (Human) 
2021-09-10 | PXD024996 | Pride
We present a study of hepatocellular carcinoma developed on non-fibrotic liver (nfHCC) that combines complementary quantitative iTRAQ-based proteomics and phosphoproteomics approaches. Using both approaches, we analyzed a set of 24 samples (18 nfHCC vs 6 non-tumor liver tissue).
ORGANISM(S): Homo sapiens (Human) 
2014-09-18 | PXD001253 | Pride
In the field of quantitative proteomics, the Isobaric Tags for Relative & Absolute Quanti-tation (iTRAQ) technology has demonstrated efficacy for proteome monitoring despite its lack of a consensus for data handling. In this study, after peptide and protein identification, we com-pared the widesprea...
ORGANISM(S): Sus scrofa domesticus (domestic pig) 
2019-08-19 | PXD007891 | Pride
Understanding the Immune Mechanisms of Vibrio mimicus targeted epitopes gene vaccine by iTRAQ Technology
ORGANISM(S): Ctenopharyngodon idella 
2018-12-18 | PXD012011 | Pride
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