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ProteomicsLC-MS/MS of human MFN2 interaction proteins
LC-MS/MS of human MFN2 interaction proteins from 293T cells or 786-O cells
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD033112 | Pride
Mfn2 Human Lc-ms/ms 
ProteomicsImmunoprecipitation of PTEN coupled Nano-LC−ESI-MS/MS
Nuclear localization of PTEN is essential for its tumor suppressive role, and loss of nuclear PTEN is more prominent than cytoplasmic PTEN in many kinds of cancers. However, nuclear PTEN-specific regulatory mechanisms were rarely reported. Based on the finding that nuclear PTEN is more unstable than...
ORGANISM(S): Homo sapiens (Human) 
2020-04-22 | PXD017983 | Pride
Immunoprecipitation Pten Nano-lc−esi-ms/ms 293t 
ProteomicsHuman 293T -cGAS-MYO1F-KAT2A,LC-MS
To identify cGAS acetylation sites, 293T cells coexpressing the PCDH-EGFP-puro empty vector or PCDH-EGFP-puro-Flag-cGAS with KAT2A and MYO1F were subjected to immunoprecipitation with an anti-Flag antibody. After washing four times with IP buffer, the protein was eluted with IP buffer containing 0.1...
ORGANISM(S): Homo sapiens (Human) 
2024-12-14 | PXD057694 | Pride
Lc-ms Human 293t -cgas-myo1f-kat2a 
ProteomicsMspire-Simulator: LC-MS shotgun proteomic simulator for creating realistic gold standard data - HEK cell lysate
The most important step in any quantitative proteomic pipeline is feature detection (aka peak picking). However, generating quality hand-annotated data sets to validate the algorithms, especially for lower abundance peaks, is nearly impossible. An alternative for creating gold standard data is to si...
ORGANISM(S): Homo sapiens (Human) 
2020-01-24 | PXD000477 | Pride
Human Ms-simulator Hek-293t Fasp 
ProteomicsLC-MS/MS of Rab21 pull-down or control sample
LC-MS/MS of Rab21 pull-down or control sample from 786-O cells
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD033104 | Pride
Human Rab21 Lc-ms/ms 
ProteomicsIndentification of CaMKK2-binding proteins in 293T cells
Indentification of CaMKK2-binding proteins under cystine starvation condition in 293T cells.
ORGANISM(S): Homo sapiens (Human) 
2021-07-24 | PXD027017 | Pride
293t cells Camkk2 
ProteomicsPotential binding partners of NUDT21 in 293T and TE-1 cells
To explore the role of NUDT21 in the progression of ESCC, we performed NUDT21 immunoprecipitation in 293T and TE1 cells following LC-MS/MS analysis.
ORGANISM(S): Homo Sapiens 
2024-12-16 | PXD058885 |
ProteomicsShotgun analysis of proteins in NDUFA11 KO and WT HEK 293T cells.
The effect of the depletion of an ETC complex I protein component - NDUFA11 on the proteome in HEK 293T cells was studied. TMT-based relative quantification of protein levels were performed in NDUFA11 KO vs WT cells and in NDUFA11 KO vs WT cells that expressed an EGFP-MAPT (Tau protein) construct. I...
ORGANISM(S): Homo sapiens (Human) 
2023-07-03 | PXD038397 | Pride
Ndufa11 ko Egfp-mapt expression Etc complex i Hek 293t 
UnknownResveratrol regulates Hsp60 in HEK 293T cells during activation of SIRT1 revealed by nascent protein labeling strategy.
Not available
S-EPMC9034730 | biostudies-literature
ProteomicsHEK-293T LC-MS/MS
We established two in vitro cell-free systems to mimic the activation of mTORC1 and autophagy by monitoring the translocation of two readout proteins Raptor and ATG14. Based on the two systems, we further screened the potential regulators involved in the leucine-mediated mTORC1 and autophagy pathway...
ORGANISM(S): Homo sapiens (Human) 
2022-03-03 | PXD007811 | Pride
Human Cell-free system Lc-ms/ms 
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