Sort   by:  
 Page size 
UnknownDetection of ADP-Ribosylation of the Androgen Receptor Using the Recombinant Macrodomain AF1521 from Archaeoglobus fulgidus.
Not available
S-EPMC7825248 | biostudies-literature
ProteomicsADP-ribosylation site analysis
We have used the Af1521 macrodomain to enrich for ADP-ribosylated peptides in human cells and mammalian tissues, and identified the individuail modification sites by LC-MS/MS
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2016-10-04 | PXD004245 | Pride
Human cell culture and maouse liver tissue 
UnknownThe ARH and Macrodomain Families of α-ADP-ribose-acceptor Hydrolases Catalyze α-NAD<sup><b>+</b></sup> Hydrolysis.
Not available
S-EPMC8388552 | biostudies-literature
ProteomicsDeciphering cytokine-driven ADP-ribosylation signaling networks via Af1521-based mass spectrometry analysis of labile Glu/Asp-linkages
ADP-ribosylation (ADPr) is a regulatory post-translational modification targeting nine amino acid residues, but glutamate/aspartate-linked ADPr (Glu/Asp-ADPr) is labile and remains challenging to detect using conventional mass spectrometry (MS)-based workflows. Using synthetic peptides, we show that...
ORGANISM(S): Homo sapiens (Human) 
2026-05-27 | PXD070310 | Pride
ProteomicsPARP14 is a PARP with both ADP-ribosyl transferase 1 and hydrolase activities
PARP14 is a mono-ADP-ribosyl transferase involved in the control of immunity, transcription and DNA replication stress management. However, little is known about the ADP-ribosylation activity of PARP14, including its substrate specificity or how PARP14-dependent ADP-ribosylation is reversed. Here we...
ORGANISM(S): Homo sapiens (Human) 
2023-08-14 | PXD043452 | Pride
Innate immunity Dna-repair Parp13 Sars-cov Af1521-interactome Ubiquitin signaling Adpr hydrolase Parp9 Adp-ribosylation Parp14 Mono-adpr Antiviral response 
ProteomicsTemporal and site-specific ADP-ribosylation dynamics upon different genotoxic stresses
The DNA damage response revolves around transmission of information via post-translational modifications, including reversible protein ADP-ribosylation. Here, we applied a mass spectrometry-based Af1521 enrichment technology for the identification and quantification of ADP-ribosylation sites as a fu...
ORGANISM(S): Homo sapiens (Human) 
2021-11-04 | PXD028902 | Pride
Proteomics Mass spectrometry Parp Post-translational modification Dna damage Af1521 Adp-ribosylation 
ProteomicsThe regulatory landscape of the human HPF1- and ARH3-dependent ADP-ribosylome (part 1)
Despite the involvement of Poly(ADP-ribose) polymerase-1 (PARP1) in many important biological pathways, the target residues of PARP1-mediated ADP-ribosylation remain ambiguous. To explicate the ADP-ribosylation regulome, we analyze human cells depleted for key regulators of PARP1 activity, histone P...
ORGANISM(S): Homo sapiens (Human) 
2021-10-07 | PXD023835 | Pride
Parp1 Adpr Hpf1 Arh3 Par Parg Af1521 Mar Adp-ribosylation 
ProteomicsPARP1 auto-modification promotes faithful Okazaki fragment processing and limits replication fork speed
Poly(ADP-ribose) polymerase (PARP) inhibitors have proven their efficacy for treating tumors defective in homologous recombination via synthetic lethality. In response to DNA breaks, PARP1 is the primary ADP-ribosylation writer, modifying itself (auto-modification) and other proteins to facilitate r...
ORGANISM(S): Homo sapiens (Human) Xenopus laevis (African clawed frog) 
2025-10-02 | PXD052191 | Pride
Okazaki fragment processing Parp1 Dna repair Af1521 macrodomain Adp-ribosylation Xenopus egg extract Auto-modification Dna replication 
ProteomicsTankyrase-mediated ADP-ribosylation is a novel regulator of TNF-induced death
TNF is a key component of the innate immune response. Upon binding to its receptor, TNFR1, it promotes production of other cytokines via a membrane-bound complex 1, or induces cell death via a cytosolic complex 2. To understand how TNF-induced cell death is regulated we performed mass spectrometry o...
ORGANISM(S): Mus musculus (Mouse) 
2022-06-09 | PXD032131 | Pride
Tnf-induced cell death Tankyrase Adp-ribosylation 
UnknownIdentification of the Mouse T Cell ADP-Ribosylome Uncovers ARTC2.2 Mediated Regulation of CD73 by ADP-Ribosylation.
Not available
S-EPMC8421852 | biostudies-literature
Sort   by:  
 Page size