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ProteomicsMass spectrometry analysis of unconventional kinetoplastid kinetochore proteins KKT1, KKT2, and KKT6
The kinetochore is the macromolecular protein machine that drives chromosome segregation in eukaryotes. Here we performed chemical crosslinking mass spectrometry of the Trypanosoma brucei KKT2 DPB/KKT1C complex and KKT1/KKT6 complex. We also performed immunoprecipitation and mass spectrometry of KKT...
ORGANISM(S): Trypanosoma brucei 
2022-06-10 | PXD034039 | Pride
Kkt6 Kkt2 Kkt1 Trypanosoma brucei Kinetoplastid kinetochore 
ProteomicsMass spectrometry analysis of the kinetoplastid kinetochore protein KKT14 phosphorylated by the Aurora B kinase
We performed mass spectrometry of a recombinant KKT14 N-terminal fragment that was phosphorylated by AUK1 (Aurora B homolog in Trypanosoma brucei) in vitro to identify phosphorylation sites.
ORGANISM(S): Trypanosoma brucei 
2024-01-31 | PXD048677 | Pride
Kkt14 Kinetochore Trypanosome Phosphorylation Kinetoplastid 
ProteomicsCrosslinking mass spectrometry of the unconventional kinetoplastid kinetochore proteins KKT16-KKT17-KKT18
The kinetochore is the macromolecular protein machine that drives chromosome segregation in eukaryotes. Here we performed crosslinking mass spectrometry of the Trypanosoma brucei KKT16 complex that consists of KKT16, KKT17, and KKT18 kinetochore proteins.
ORGANISM(S): Trypanosoma brucei 
2021-04-07 | PXD025220 | Pride
Trypanosoma brucei Kinetochore Kinetoplastid 
ProteomicsCrosslinking mass spectrometry of the unconventional kinetoplastid kinetochore protein KKT4
The kinetochore is the macromolecular protein machine that drives chromosome segregation by interacting with spindle microtubules. Unlike most other eukaryotes that have canonical kinetochore proteins, a group of evolutionarily divergent eukaryotes called kinetoplastids (such as Trypanosoma brucei t...
ORGANISM(S): Trypanosoma brucei 
2020-10-15 | PXD020229 | Pride
Trypanosoma brucei Kinetoplastid Kkt4 Kinetochore 
ProteomicsMass spectrometry analysis of chromosomal passenger complex proteins and kinetochore proteins in Trypanosoma brucei
We performed chemical crosslinking mass spectrometry of native protein complexes purified from Trypanosoma brucei procyclic cells to obtain protein-protein interaction information for the chromosomal passenger complex (CPC) and kinetochore complex. Crosslinks among CPC subunits were detected using Y...
ORGANISM(S): Trypanosoma brucei 
2023-10-13 | PXD045987 | Pride
Kinetochore Crosslinking mass spectrometry 
ProteomicsMass spectrometry analysis of kinetoplastid kinetochore proteins (KKT23 acetyltransferase and its interactor KKT22) and histone acetylation levels in Trypanosoma brucei
KKT23 is a kinetoplastid kinetochore protein that has structural similarity to the GCN5 acetyltransferase domain. Using a kinetoplastid parasite Trypanosoma brucei, here we performed immunoprecipitation and mass spectrometry of GFP-tagged KKT23 N-terminal fragment (2–150) to identify co-purifying pr...
ORGANISM(S): Trypanosoma brucei 
2024-08-19 | PXD052922 | Pride
Trypanosome kinetoplastid kinetochore kkt23 gcn5 
ProteomicsCrosslinking mass spectrometry analysis of the Trypanosoma brucei KKT23 complex
KKT23 is a kinetoplastid kinetochore protein that has structural similarity to the GCN5 acetyltransferase domain. We performed crosslinking mass spectrometry of the recombinant KKT3-KKT22-KKT23 complex purified from Sf9 insect cells.
ORGANISM(S): Trypanosoma brucei 
2024-08-19 | PXD052923 | Pride
Trypanosome kinetoplastid kinetochore kkt23 gcn5 
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