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ProteomicsStructural and functional differentiation of the light-harvesting protein Lhcb4 during land plant diversification
About 475 million years ago plants originated from an ancestral green alga and evolved first as non-vascular and later as vascular plants, becoming the primary producers of biomass on lands. During that time, the light harvesting complex II (LHCII), responsible for sunlight absorption and excitation...
ORGANISM(S): Pisum sativum (Garden pea) 
2019-03-25 | PXD011213 | Pride
Proteomics Light acclimation Swath Psii Land plants evolution Label-free Lhcii Light harvesting 
ProteomicsThe synergy between plastid and mitochondria modulates responses to iron limitation in two Thalassosira diatom species
Iron (Fe) is an essential element for life. Therefore, oceanic phytoplankton must adapt to the naturally low iron levels in modern oceans. Diatoms are a highly diverse and successful phytoplankton taxon that accounts for around 20% of global primary production. Previous studies have shown that they ...
ORGANISM(S): Thalassiosira oceanica Thalassiosira pseudonana 
2026-09-28 | PXD071344 | Pride
Diatoms Photosynthesis Iron limitation Mitochondria Thalassiosira Chloroplasts 
ProteomicsDeciphering Photosynthetic Protein Networks: A Crosslinking-MS Strategy for Studying Functional Thylakoid Membranes
Photosynthesis relies on a dynamic network of protein-protein interactions that adapt to fluctuating light conditions. We present an XL-MS pipeline applied directly to functional thylakoid membranes from Arabidopsis thaliana and Spinacia oleracea. By combining PhoX crosslinker with the amphiphilic s...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Spinacia sp. 
2026-05-14 | PXD068425 | Pride
Arabidopsis thaliana Crosslinking-ms Thylakoid membranes Photosynthesis Protein-protein interactions Structural proteomics Spinacia oleracea 
ProteomicsThe phytoplasma effector Phyllogen structurally mimics host plant MADS transcription factors
Phytoplasmas are obligate plant pathogens that include species causing severe developmental abnormalities in diverse plant families. Phytoplasma infections may result in dwarfism, witches’ broom, virescence and phyllody, the conversion of reproductive organs into leaf-like structures. Phyllody is du...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-09-21 | PXD074375 | Pride
Transcription factor Arabidopsis Crosslinking 
ProteomicsPhotoreceptor-induced LHL4 protects photosystem II in Chlamydomonas reinhardtii - BN-PAGE profiling
Photosynthesis, the fundamental process using light energy to convert CO2 to organic matter, is vital for life on Earth. It relies on capturing light through light-harvesting complexes in photosystems I and II and converting light energy into chemical energy. Composition and organization of photosys...
ORGANISM(S): Chlamydomonas reinhardtii 
2025-01-28 | PXD055508 | Pride
In-gel crosslikning Photosystem ii Dmtmm Light-harvesting complexes Xl-ms Thylakoids 
ProteomicsXL-MS of of influenza A NEP/NS2
The influenza nuclear export protein (NEP)/non-structural protein 2 (NS2) is a multifunctional protein, involved in viral ribonucleoprotein (vRNP) export from the nucleus, genome replication enhancement, and the adaption of avian influenza to mammals. Despite the growing attention on the importance ...
ORGANISM(S): Influenza A virus (A/Philippines/TMC10-144/2010(H1N1)) 
2026-02-12 | PXD068009 | Pride
Sda Influenza Photo-crosslinking Virus Crosslinking 
ProteomicsPhotoreceptor-induced LHL4 protects photosystem II in Chlamydomonas reinhardtii
Photosynthesis, the fundamental process using light energy to convert CO2 to organic matter, is vital for life on Earth. It relies on capturing light through light-harvesting complexes in photosystems I and II and converting light energy into chemical energy. Composition and organization of photosys...
ORGANISM(S): Chlamydomonas reinhardtii 
2025-01-28 | PXD049352 | Pride
In-gel crosslikning Photosystem ii Dmtmm Light-harvesting complexes Xl-ms Thylakoids 
ProteomicsThylakoid membrane proteome remodelling in response to variation in plant growth irradiance
Plant thylakoid membranes contain hundreds of proteins closely interplaying to cope with ever-changing environmental conditions. We investigated how P. sativum (pea) grown at different irradiances optimizes light-use efficiency through the differential accumulation of thylakoid proteins. Thylakoid m...
ORGANISM(S): Pisum sativum (Garden pea) 
2018-10-02 | PXD009448 | Pride
Thylakoid membrane Swath analysis Photosynthesis Quantitative proteomics Light acclimation 
ProteomicsHow paired PSII-LHCII supercomplexes mediate the stacking of plant thylakoid membranes unveiled by integrative structural mass-spectrometry
Photosynthesis drives all life on Earth by exploiting solar energy to split water molecules through the Photosystem (PS) II enzyme. In plant thylakoid membranes, PSII binds a modular set of light harvesting complexes (LHCII) to form different types of PSII-LHCII supercomplex (PSII-LHCIIsc). Plant PS...
ORGANISM(S): Pisum sativum (Garden pea) 
2020-03-19 | PXD017382 | Pride
Plant proteomics Top-down rp-lc-ms/ms Acetylation Dsso Psii Edc Photosystem Cx-ms Crosslinking Chlorophyll-binding proteins 
ProteomicsThriving in the heat – Lysine acetylation stabilizes the quaternary structure of a Mega-Dalton hyperthermoactive PEP-synthase
Over time structural adaptations enabled proteins and enzymes to have sufficient stability and flexibility to perform the basic functions of life under various environmental conditions. The catalytic cores of key metabolic enzymes of hyperthermophilic archaea work at a temperature range of 80-120 °C...
ORGANISM(S): Pyrococcus furiosus DSM 3638 
2022-08-08 | PXD035847 | Pride
Structural proteomics Protein complex stability Lysine acetylation Molecular dynamics Cryo-em Acylation 
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