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ProteomicsDevelopment of a high-pH reversed-phase well plate for peptide fractionation and deep proteome analysis of cells and exosomes
Development of a high-pH reversed-phase well plate for peptide fractionation and deep proteome analysis of cells and exosomes
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-07-21 | MSV000087860 | MassIVE
ProteomicsSite-specific O-Glycosylation Analysis of Human Blood Plasma Proteins
Here, we report on the site-specific O-glycosylation analysis of human blood plasma glycoproteins. To this end pooled human blood plasma of healthy donors was digested non-specifically using Protein-ase K, followed by a precipitation step, as well as a glycopeptide enrichment and fractionation step ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2015-06-05 | MSV000079141 | MassIVE
ProteomicsSingle amino acid variant
We performed deep proteomic profiling down to as few as 9 Panc-1 cells using sample fractionation, TMT multiplexing and carrier/reference strategy. Off line fractionation of the TMT-labeled sample pooled with TMT-labeled carrier Panc-1 whole cell proteome was achieved using alkaline reversed phase s...
ORGANISM(S): Homo sapiens (Human) 
2020-05-26 | PXD011471 | Pride
Human Panc-1 Lc-ms/ms 
ProteomicsMolecular basis of b-adrenergic regulation of cardiac calcium current
Increased cardiac contractility during fight-or-flight response is caused by b-adrenergic augmentation of CaV1.2 channels. It is assumed that this iconic regulation involves phosphorylation of CaV1.2 a1/b-subunits. In transgenic murine hearts expressing fully PKA phosphorylation-deficient mutant a1C...
ORGANISM(S): Mus musculus (Mouse) 
2019-11-19 | PXD014501 | Pride
Isobaric labeling Isoproterenol Tmt Alkaline reversed phase peptide fractionation Non-transgenic Cardiomyocytes Quantitative mass spectrometry Sps ms3 B-adrenergic regulation 
ProteomicsExpanding the cerebrospinal fluid endopeptidome
Biomarkers of neurodegenerative disorders are needed to assist in diagnosis, to monitor disease progression and therapeutic interventions, and to provide insight into disease mechanisms. One route to identify such biomarkers is by proteomic and peptidomic analysis of cerebrospinal fluid (CSF). In t...
ORGANISM(S): Homo sapiens (Human) 
2017-01-17 | PXD004863 | Pride
Peptidomics Alzheimer’s disease Cerebrospinal fluid Peptides Parkinson’s disease Biomarkers Neurodegeneration 
ProteomicsEvaluation of disposable-trap column nanoLC-FAIMS-MS/MS for the proteomic analysis of FFPE tissue
Proteomic biomarker discovery using formalin-fixed paraffin-embedded (FFPE) tissue requires robust workflows to support the analysis of large cohorts of patient samples. It also requires finding a reasonable balance between achieving high proteomic depth and limiting overall analysis time. To this e...
ORGANISM(S): Homo sapiens (Human) 
2021-11-02 | PXD028136 | Pride
Human Quantitative proteomics High field asymmetric waveform ion mobility spectrometry (faims) Ffpe Clinical proteomics Lc-faims-ms/ms 
ProteomicsA Spatiotemporal Map of Co-Receptor Signaling Networks Underlying B Cell Activation
The B cell receptor (BCR) signals together with a multi-component co-receptor complex to initiate B cell activation in response to antigen binding. This process underlies nearly every aspect of proper B cell function. Here, we take advantage of peroxidase-catalyzed proximity labeling combined with q...
ORGANISM(S): Homo sapiens (Human) 
2024-06-06 | PXD040262 | Pride
Systems immunology Proteomics Bcr signaling B cell co-receptor B cells Phosphoproteomics 
ProteomicsMolecular basis of b-adrenergic regulation of cardiac calcium current
Increased cardiac contractility during fight-or-flight response is caused by b-adrenergic augmentation of CaV1.2 channels. It is assumed that this iconic regulation involves phosphorylation of CaV1.2 a1/b-subunits. In transgenic murine hearts expressing fully PKA phosphorylation-deficient mutant a1C...
ORGANISM(S): Mus musculus (Mouse) 
2019-11-19 | PXD014500 | Pride
Streptavidin Apex Apex2 Alkaline reversed phase peptide fractionation H2o2 Denaturing purification Sps ms3 Cardiac calcium channel B-adrenergic regulation Isobaric labeling Isoproterenol Tmt Proximity labeling Biotin-phenol Quantitative mass spectrometry Cav1.2 Biotin-tyramide On-bead digest Peroxidase catalyzed 
ProteomicsDiscovery of covalent CDK14 inhibitors with pan-TAIRE family specificity
Cyclin dependent kinase 14 (CDK14) and other TAIRE family kinases (CDKs 15-18) are proteins that lack functional annotation but are frequent off-targets of clinical kinase inhibitors. In this study we develop and characterize FMF-04-159-2, the first tool compound that specifically targets CDK14 cova...
ORGANISM(S): Homo sapiens (Human) 
2019-03-29 | PXD012692 | Pride
Covalent inhibitor Druggable genome Mitosis Taire kinase Cell-cycle Cdk14 
ProteomicsPhoXplex: Combining phospho-enrichable crosslinking with isobaric labelling for quantitative proteome-wide mapping of protein interfaces
Integrating crosslinking mass spectrometry (XL-MS) into structural biology workflows provides valuable information about the spatial arrangement of amino acid stretches, which can guide elucidation of protein assembly architecture. Additionally, combination of XL-MS with peptide quantitation techniq...
ORGANISM(S): Homo sapiens (Human) 
2024-10-21 | PXD052480 | Pride
Isobaric labelling Phospho-enrichable crosslinking 
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