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TranscriptomicsB-catenin deficiency, but not c-Myc deletion, suppresses the immediate phenotypes of Apc loss in the liver
Dysregulated Wnt signalling is seen in approximately 30% of hepatocellular cancers, thus finding pathways downstream of activation of Wnt signalling is key. Using cre lox technology we have deleted the the adenomatous polyposis coli tumour suppressor protein (Apc) within the adult mouse liver and ob...
ORGANISM(S): Mus musculus 
2015-01-31 | E-GEOD-65476 | biostudies-arrayexpress
ProteomicsProteomic profiling of Palbociclib arrested cells ± APC/C inactivation.
Cellular proliferation is highly regulated to ensure proper tissue homeostasis and to prevent diseases such as cancer. In actively proliferating cells, entry into S phase of the cell cycle and initiation of DNA replication is promoted by inactivation of the E3 ubiquitin ligase APC/C (anaphase promot...
ORGANISM(S): Homo sapiens (Human) 
2024-07-18 | PXD046783 | Pride
Cancer Palbociclib Cell cycle Apc/c 
ProteomicsSpatial control of the APC/C ensures the rapid degradation of Cyclin B1
The proper control of mitosis depends on the ubiquitin-mediated degradation of the right mitotic regulator at the right time. This is under the control of the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase that is regulated by the Spindle Assembly Checkpoint (SAC). The Checkpoint prev...
ORGANISM(S): Homo sapiens (Human) 
2025-05-07 | PXD052677 | Pride
Apc/c Cyclin b1 
ProteomicsSUMO targets the APC/C in a phosphorylation-dependent manner to regulate transition from metaphase to anaphase
Signal transduction by Small Ubiquitin-like Modifier (SUMO) regulates a myriad of nuclear processes. Here, we report on the role of SUMO in mitosis in human cell lines. Knocking down the SUMO conjugation machinery resulted in a delay in mitosis and defects in mitotic chromosome separation. Searching...
ORGANISM(S): Homo sapiens (Human) 
2018-09-19 | PXD006335 | Pride
Anaphase promoting complex Apc4 Sumo Mitosis Phosphorylation Chromatin bridges Kif18b Ubiquitin 
ProteomicsBS3 crosslinking of the Anaphase-Promoting Complex/Cyclosome (APC/C)
In order to investigate the dynamic regulation of the APC/C by intrinsically disordered regions (IDRs) upon phosphorylation, we employed cross-linking mass spectrometry (CLMS). Given that the phosphorylation of Apc1-300L (IDR in Apc1) is a key determinant in its release from the co-activator binding...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2024-04-23 | PXD050097 | Pride
Crosslinking mass spectrometry The anaphase-promoting complex/cyclosome (apc/c) 
ProteomicsSpatial control of the APC/C ensures the rapid degradation of Cyclin B1
The proper control of mitosis depends on the ubiquitin-mediated degradation of the right mitotic regulator at the right time. This is under the control of the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase that is regulated by the Spindle Assembly Checkpoint (SAC). The Checkpoint prev...
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD046458 | Pride
Ubiquitin-mediated degradation Mitosis Spindle assembly checkpoint Human 
ProteomicsBS3 crosslinking of the Anaphase-Promoting Complex/Cyclosome (APC/C)
How Apc8-LOOP affects the displacement of Apc1-300LOOP was investigated using crosslinking mass spectrometry (MS) to gain insight into the point of interaction between disordered Apc8-LOOP and the APC/C center structure.
ORGANISM(S): Xenopus laevis (African clawed frog) 
2024-09-08 | PXD044827 | Pride
Crosslinking mass spectrometry Disordered loop domains Phosphorylation Apc/c 
ProteomicsMps1(Mph1) Kinase phosphorylates Mad3 to Inhibit Cdc20Slp1 –APC/C and maintain spindle checkpoint arrests
The spindle checkpoint is a mitotic surveillance system which ensures equal segregation of sister chromatids. It delays anaphase onset by inhibiting the action of the E3 ubiquitin ligase known as the anaphase promoting complex or cyclosome (APC/C). Mads/BubR1 is a key component of the mitotic chec...
ORGANISM(S): Schizosaccharomyces pombe 
2016-03-31 | PXD003806 | Pride
Mad3 Kinase Mitosis Spindle Phosphorylation S.pombe 
ProteomicsKNL2 degradation is regulated by APC/C ubiquitin ligase
Centromeres are specialized chromosomal regions where kinetochore proteins form a protein complex that regulates the cell cycle. KNL2 is an essential protein for kinetochore assembly and plays a significant role in the loading of CENH3. Therefore, the level of KNL2 in plants and other organisms need...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2025-07-14 | PXD051741 | Pride
Kinetochore null2 Centromeres E3 ligases E2 conjugating enzymes Protein-protein interactions Apc/c D-box 
ProteomicsCdh1 Proteomic Analysis - A Synthetic Lethal Interaction between APC/C and Topoisomerase Poisons Uncovered by Proteomic Screens
The Anaphase-promoting complex/cyclosome (APC/C) cofactor Cdh1 modulates proliferation and cell cycle exit by targeting multiple cell cycle regulators for ubiquitin-dependent degradation. Lack of Cdh1 results in structural and numerical chromosome aberrations, a hallmark of genomic instability, alth...
ORGANISM(S): Mus musculus (Mouse) 
2014-02-20 | PXD000511 | Pride
Ubiquitination Cdh1 Silac Mouse Apc/c Cell cycle Itraq 
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