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ProteomicsLC-MS/MS analysis of NAA15 and NAA10 IP
LC-MS/MS analysis of endogenous NAA15 and NAA10 immunoprecipitated from HeLa cell lysates was performed to identify cellular binding partners.
ORGANISM(S): Homo sapiens (Human) 
2025-12-17 | PXD059724 | Pride
N-terminal acetylation Nata Naa15 Naa10 
ProteomicsLC-MSMS analysis of immunoprecipitated JunB-FLAG from HAP1 ADO KO cells
Define the N-terminal acetylation status of human JunB in siCTRL, siNAA10 or siNAA20 treated HAP1 ADO KO cells.
ORGANISM(S): Homo sapiens (Human) 
2024-07-03 | PXD045695 | Pride
Lc-msms Human Junb 
ProteomicsN-terminal acetyaltion of Human JunB in yeast expressing all N-terminal acetyltransferases, no NatA enzyme or no NatB enzyne
Define the N-terminal status of overexpressed human JUNB in WT yeast strain, NAA10 Δ strain and NAA20 Δ strain.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-07-03 | PXD047612 | Pride
Junb N-terminal acetyltransferase 
ProteomicsDetermining the hydroxylation status of NAA10-Trp38
NAA10 is the major human N-terminal acetyltransferase (NAT). The KAT activity of NAA10 towards hypoxia-inducible factor 1α (HIF-1α) was recently reported to depend on the hydroxylation at Trp38 of NAA10 by factor inhibiting HIF-1α (FIH). As a consequence, Trp38 hydroxylation status was proposed to a...
ORGANISM(S): Homo sapiens (Human) 
2022-02-16 | PXD023655 | Pride
Hydroxylation Asp-oh Asn-oh Naa10 Trp-oh Nat 
ProteomicsLC-MS/MS analysis of immunoprecipitated NAA15-V5 variants
The human N-terminal acetyltransferase E (NatE) including its associated NatA co-translationally acetylates the N-terminus of about 40-60% of the proteome to mediate diverse biological processes including protein half-life, localization and protein interaction. In eukaryotes, the NatE complex contai...
ORGANISM(S): Homo sapiens (Human) 
2020-03-13 | PXD017031 | Pride
Lc-ms/ms N-terminal acetylation Nate Naa15 Naa10 Hypk Naa50 
ProteomicsNAA80-V5 interactor screen in HeLa and HAP1 cells
Actin is N-terminally acetylated by NAA80. We aimed to characterize the NAA80 interactome.
ORGANISM(S): Homo sapiens (Human) 
2020-09-25 | PXD021408 | Pride
N-terminal acetylation Cytoskeleton Acetylation Actin Protein modifications Nat Naa80 
ProteomicsLC-MS/MS shotgun proteome analysis of Saccharomyces cerevisiae lysates
N-terminal acetylation is a highly abundant protein modification in eukaryotes which impacts a wide range of cellular processes and protein properties. This modification was proven to be essential for development and viability of multiple eukaryotes. Still the molecular mechanisms at play and the pr...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2018-11-15 | PXD009214 | Pride
Tandem mass spectrometry Saccharomyces cerevisiae N-terminal acetylation 
ProteomicsNaa80 acetylates the actin N-terminus impacting the actin cytoskeleton and cell motility
Eukaryotic actins undergo several post-translational modifications during their maturation to enable a fully functional cytoskeletal network. A unique N-terminal processing is conserved in the animal kingdom and includes the removal of the first one or two amino acids as well as N-terminal acetylati...
ORGANISM(S): Homo sapiens (Human) 
2018-10-24 | PXD006856 | Pride
N-terminal acetylation Nat Actin Human 
ProteomicsmNaa10-KO liver immunoprecipitation
NAA10-mediated N-terminal acetylation is widespread and has been considered essential for viability in many model organisms. However, a Naa10 null mouse model is viable and has intact global N-terminal acetylation levels. The purpose of this project was to assess the N-terminal acetyltransferase act...
ORGANISM(S): Mus musculus (Mouse) 
2021-08-17 | PXD026684 | Pride
N-terminal acetylation Nta Naa10 Naa12 Nat 
UnknownCharacterization of hARD2, a processed hARD1 gene duplicate, encoding a human protein N-alpha-acetyltransferase.
Not available
S-EPMC1475586 | biostudies-literature
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