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ProteomicsOCT1 (SLC22A1) transporter kinetics and regulation in primary human hepatocyte 3D spheroids
3D spheroids of primary human hepatocytes (3D PHH) keep a differentiated phenotype with retained metabolic function and proteome fingerprint for weeks in culture. As a result, 3D PHH are gaining ground as a model for mechanistic studies on liver homeostasis and in vitro to in vivo (IVIV)predictions...
ORGANISM(S): Homo sapiens (Human) 
2024-08-10 | PXD045733 | Pride
Human Hepatocytes Adme Liver 
ProteomicsThe intracellular free concentration of EDCs enables successful translation between cell-free and cell-based estrogenic activity assays
Many environmental toxicants can activate the estrogen receptor α (ERα), contributing to disruption of normal endocrine function. Although these activities are known from in silico, in vitro and in vivo models, transferring the active concentrations between the different models is often challenging....
ORGANISM(S): Homo sapiens (Human) 
2025-07-31 | PXD059544 | Pride
In vitro Endocrine-disrupting chemicals Estrogenic chemicals Intracellular free concentration 
ProteomicsHuman liver homogenate, liver microsomes, low-speed centrifugation pellet and hepatocytes LC-MS/MS from 15 matched donors
LC-MS/MS-based label-free proteomics of human liver homogenate, human liver microsomes, and human hepatocytes from 15 matched donors used to compare and understand differences in two major in vitro systems used for drug metabolism studies
ORGANISM(S): Homo sapiens (Human) 
2021-03-29 | PXD014131 | Pride
Human Low-speed centrifugation pellet Hepatocytes Liver Liver microsomes Lc-ms/ms 
ProteomicsLiver zonation patterns and cell size variability in isolated human hepatocytes
Human hepatocytes differ in gene expression and function across the hexagonal lobules of the tissue microarchitecture, a phenomenon referred to as liver zonation. Hepatocytes also display intra-lobular differences in cell size, but to what extent zonal expression and function correlate with cell siz...
ORGANISM(S): Homo sapiens (Human) 
2021-01-26 | PXD013142 | Pride
Human Hepatocytes Cell size variability Liver Zonation 
ProteomicsConditions for maintenance of hepatocyte differentiation and function in 3D cultures
3D spheroid cultures of primary human hepatocytes (PHH) are used in studies of hepatic drug metabolism and toxicity. However, the 3D spheroids are maintained under different conditions, with possible cofounding results. Here we performed an in-depth analysis of how various culture conditions influen...
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD024632 | Pride
Primary human hepatocyte culture Lc-ms/ms 
ProteomicsProteomic Profiling Reveals Age-Related Changes in Transporter Proteins in the Human Blood-Brain Barrier
The Blood-Brain Barrier (BBB) is a crucial, selective barrier that regulates the entry of molecules including nutrients, environmental toxins, and therapeutic medications into the brain. This function relies heavily on brain endothelial cell proteins, particularly transporters and tight junction pro...
ORGANISM(S): Homo sapiens (Human) 
2025-11-28 | PXD057192 | Pride
Human Lc-msms Brain microvessels Blood-brain barrier Transporters Maxquant Orbitrap Aging Ontogeny 
ProteomicsProteomics informed deconvolution of 3 cell lines
The project aimed to investigate the possibility to use proteomics data to deconvolute cell line proportions in mixed samples. Samples containing either HEK 293, Caco-2, or A549 cells and mixtures of the three cell lines was analysed using the total protein approach. This was then used for proteomic...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD027282 | Pride
Hek 293 A549 Caco-2 
ProteomicsIlluminating the Function of the Orphan Transporter, SLC22A10 in Humans and Other Primates
HEK293 Flp-In cells were transfected transiently with various SLC22A10 orthologs, including human, chimpanzee, and the mutations to proline or leucine at position 220. After 72 hours of transfection, cell pellets were collected. The quantification was performed on both HEK293 cells and HEK293cells e...
ORGANISM(S): Homo sapiens (Human) 
2024-04-09 | PXD047102 | Pride
Hek293 Slc22a10 Lc-ms/ms 
ProteomicsHuman Hepatocyte Proteome
Here we report a comprehensive proteomic analysis of purified human hepatocytes and a human hepatoma cell line HepG2. The complete dataset comprises 9,400 proteins and provides quantitative depiction of the proteomes of hepatocytes and HepG2 cells at the protein titer and copy number dimensions.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-03-07 | MSV000079562 | MassIVE
MetabolomicsFirst-in-class MKK4 inhibitors enhance liver regeneration and prevent liver failure

Diminished hepatocyte regeneration is a key feature of acute and chronic liver diseases and after extended liver resections, resulting in the inability to maintain or restore a sufficient functional liver mass. Therapies to restore hepatocyte regeneration are lacking, making liver transplantation...

2024-09-23 | MTBLS9434 | MetaboLights
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