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ProteomicsNIH 3T3 AHA p-c, SILAC p-c, inhibitor, and enrichment control experiments
We developed a pulse-chase method in where fully SILAC (heavy, medium-heavy and Light) labelled cells were pulsed with Azidohomoalanine (AHA) and then chased for different length of times. These experiments were performed with 0, 1, 2, 4, 8, 16 and 13 h of chase. Data from 3 biological replicates ar...
ORGANISM(S): Mus musculus (Mouse) 
2016-10-06 | PXD004929 | Pride
Azidohomoalanine Mouse Silac Degradation Aneuploidy 
ProteomicsRPE-1 and RPE-1 trisomic AHA p-c and steady state data
We developed a pulse-chase method in where fully SILAC (heavy, medium-heavy and Light) labelled cells were pulsed with Azidohomoalanine (AHA) and then chased for different length of times. In addition, steady state protein levels comparing the RPE- and RPE-1 trisomic cells by standard SILAC labeling...
ORGANISM(S): Homo sapiens (Human) 
2016-10-06 | PXD004915 | Pride
Human Aneuploidy Pre-1 
ProteomicsHEK293T cell translatome analysis upon Torin1 treatment or single amino acid withdrawal. Related to Supplementary Table 6 from An et al (2020)
HEK293T subject to Single AA starvation dataset and translatome analysis was determined using Azidohomoalanine (AHA) incorportation, Biotin-click, and streptavidin enrichment. TMTpro 16plex labels was used.
ORGANISM(S): Homo sapiens (Human) 
2020-04-30 | PXD017861 | Pride
Human Lc-msms Azidohomoalanine (aha) 
ProteomicsAnalysis of the nascent proteome during murine norovirus infection
SILAC- and AHA-labelling analysis of Raw264.7 cells infected with MNV-1 at MOI=10TCID50/cell and harvested at 6.5 and 10.5 hours post infection. AP-MS enrichment of AHA-labelled nascent polypeptides represents the first quantitative neoproteomics analysis of norovirus infected cells.
ORGANISM(S): Mus musculus (Mouse) 
2021-07-02 | PXD025169 | Pride
Raw264.7 Aha Mnv Silac 
ProteomicsHEK293T cell translatome analysis upon 6-MP treatment. Related to Supplementary Table 6 from An et al (2020)
HEK293T cells were treated with 6-MP and harvested at various time point (6, 9 and 18h). Translatome was measure by Azidohomoalanine (AHA) incorporation, Biotin-click, and streptavidin enrichment.
ORGANISM(S): Homo sapiens (Human) 
2020-04-30 | PXD017860 | Pride
Human Lc-msms Azidohomoalanine (aha) incorporation 
ProteomicsHEK293T cell degradome (pulse chase) analysis upon 12h Torin1 treatment. Related to Supplementary Table 3 from An et al (2020)
HEK293T Azidohomoalanine (AHA) Pulse Chase (degradome) experiment, were cells from various genetic background (WT, ATG7-/-, RB1CC1-/-) are treated or not with Torin1 for 12h. Samples are Biotin-click, and streptavidin enriched.
ORGANISM(S): Homo sapiens (Human) 
2020-04-30 | PXD017857 | Pride
Human Lc-msms Azidohomoalanine (aha) incorporation 
UnknownAzidohomoalanine (AHA) Metabolic Labeling Reveals Unique Proteomic Insights into Protein Synthesis and Degradation in Response to Bortezomib Treatment.
Not available
S-EPMC12737224 | biostudies-literature
Proteomicssemi-automated enrichment of AHA-containing newly-synthesized proteins with magnetic alkyne agarose beads
Development of an improved workflow for newly synthesized proteome analysis, based on combined pulsed metabolic labelling with L-azidohomoalanine (AHA) and semi-automated click chemistry-based enrichments with magnetic alkyne agarose beads.
ORGANISM(S): Homo sapiens (Human) 
2023-10-19 | PXD036886 | Pride
Automation Silac Click chemistry Nascent proteome 
ProteomicsHEK293T cell translatome analysis upon Torin1 treatment or single amino acid withdrawal. Related to Supplementary Table 2 from An et al (2020)
HEK293T subject to Torin1 treatment or amino acid withdraw dataset and translatome analysis was determined using Azidohomoalanine (AHA) incorportation, Biotin-click, and streptavidin enrichment. TMT 10plex labelling was used. Three genotypes, WT (24 fractions), ATG7 KO (HA-9-53-1 single shot), RB1CC...
ORGANISM(S): Homo sapiens (Human) 
2020-04-30 | PXD018252 | Pride
Human Lc-msms Azidohomoalanine (aha) 
ProteomicsOptimization of protocols for detection of de novo protein synthesis in whole blood samples via azide-alkyne cycloaddition
Aberrant protein synthesis and protein expression are a hallmark of many disorders ranging from cancer Aberrant protein synthesis and protein expression are a hallmark of many disorders ranging from cancer to neuropsychiatry. Blood-based biomarkers indicative of changes in proteomes have long been h...
ORGANISM(S): Rattus norvegicus (Rat) 
2020-10-08 | PXD019243 | Pride
De novo protein synthesis Blood-based biomarkers Copper(i)-catalyzed click-chemistry Whole blood Ethylenediaminetetraacetic acid 
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