Basic reverse phase fractionation

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Acidithiobacillus ferrooxidans ATCC 53993 (266)
Aerococcus urinae NBRC 15544 = CCUG 36881 (266)
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UNIPROT ID/AC

1433A_SOYBN (2)
1433B_SOYBN (2)
1433C_SOYBN (2)
1433D_SOYBN (2)
2SS_SOYBN (2)
3847.GLYMA0042S100.1 (2)
3847.GLYMA0070S50.1 (2)
3847.GLYMA01G05321.1 (2)
3847.GLYMA01G05765.1 (2)
3847.GLYMA01G09540.1 (2)
3847.GLYMA01G09640.1 (2)
3847.GLYMA01G14160.1 (2)
3847.GLYMA01G22140.1 (2)
3847.GLYMA01G23427.1 (2)
3847.GLYMA01G25116.1 (2)
3847.GLYMA01G27160.1 (2)
3847.GLYMA01G29440.1 (2)
3847.GLYMA01G33803.1 (2)
3847.GLYMA01G35730.1 (2)
3847.GLYMA01G35820.1 (2)
3847.GLYMA01G42950.2 (2)
3847.GLYMA02G01060.2 (2)
3847.GLYMA02G01170.1 (2)
3847.GLYMA02G04090.1 (2)
3847.GLYMA02G06710.1 (2)
3847.GLYMA02G07430.1 (2)
3847.GLYMA02G07740.1 (2)
3847.GLYMA02G11920.1 (2)
3847.GLYMA02G14495.1 (2)
3847.GLYMA02G14521.1 (2)

Metabolite Name

Quercetin 3-O-Xylosyl-Rutinoside (1)
4-Morpholinepropanesulfonic Acid (1)
Glucose Lactate Acetate (1)
1-(Tert-Butylamino)-3-(3-Fluorophenyl)Thiourea (1)
Desmethylmisonidazole (1)
3,5-Dihydroxy-6-Methyl-2,3-Dihydro-4H-Pyran-4-One (1)
3-Methyl-2-Butenal (1)
2-Amino-9-[(4S)-4-Hydroxy-5-(Hydroxymethyl)Oxolan-2-Yl]-1H-Purin-6-One (1)
Feruloyl Allylamine (1)
Sulfometuron-Methyl (1)
11,13-Dehydroeriolin (1)
Rose Oxide (Cis) (1)
5,8-Dihydroxy-1,4-Naphthoquinone (1)
Hydroxy Bosentan (1)
Betalamic Acid (1)
Val-Leu-Pro-Val-Pro (1)
Monnieriside G (1)
Tetrahydropterin (1)
5-(3,4-Dimethoxyphenyl)-3,4-Dimethyloxolan-2-One (1)
1-[3-(4-Chlorophenoxy)Propyl]-2-Methyl-4-Nitroimidazole (1)
Feruloyl O-Methyldopamine (1)
4-Amino-1-[(2S,4S)-2-(Hydroxymethyl)-1,3-Oxathiolan-4-Yl]Pyrimidin-2-One (1)
7-Tert-Butyl-N-(Thiophen-2-Ylmethyl)Pyrazolo[1,5-A]Pyrimidine-5-Carboxamide (1)
7-O-Methyl Morroniside (1)
Erysenegalensein J (1)
Bornyl Ferulate (1)
Prostaglandin Pge2 1-Glyceryl Ester (1)
Phenamil (1)
Kuwanon G (1)
N-Phenethyl-3-Phenyl-Acrylamide (1)

Modification

4-tetrahydrothiazine-3-carboxylic acid (1)
4x(2)H labeled dimethylated residue (1)

Study type

Unknown experiment type (1)
Transcription profiling by tiling array (1)
Transcription profiling by array (1)
RNA-seq of non coding RNA (1)

Release Date

2020 (3)
2010 (2)
2015 (2)
2013 (1)
2007 (1)
2025 (1)
2022 (1)
2021 (1)
2019 (1)
2016 (1)

Lab affiliation

Centre for Proteome Research, University of Liverpool, Liverpool, UK (9)
Medical University of Vienna (7)
Centre for Cancer Cell & Molecular Biology, Barts Cancer Institute, Queen Mary University of London, Charterhouse Square, London EC1M 6BQ, UK. (5)
Basic Immune System of Plants / Protein Mass Spectrometry Max Planck Institute for Plant Breeding Research Carl-von-Linne-Weg 10, 50829 Cologne, Germany (4)
Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, Rhode Island, USA (4)
Principal Investigator Centre for Targeted Protein Degradation, School of Life Sciences (4)
Institut für Mikrobiologie, Universität Greifswald, Felix-Hausdorff-Str. 8, 17489 Greifswald (3)
Institute of Microbiology, Department of Microbial Proteomics, Center for Functional Genomics of Microbes, University of Greifswald, 17489 Greifswald, Germany (3)
Molecular Oncology Chemical Biology and Molecular Medicine Moffitt Cancer Center (3)
National Key Laboratory of Medical Molecular Biology & Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (3)
State Key Laboratory of Genetic Engineering, Department of Biochemistry and Biophysics, School of Life Sciences, Fudan University, Shanghai 200438, China (3)
Biochemistry and Molecular Biology, Michael Smith Laboratories, University of British Columbia, Canada (2)
CRUK Beatson Institute (2)
Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA (2)
Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, Austria (2)
Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang, 50463, Republic of Korea (2)
Harvard Medical School (2)
Harvard Medical School, Department of Cell Biology (2)
Institute of Cancer Research, London (2)
LSUHSC Proteomics Core Facility Department of Biochemistry and Molecular Biology LSU Health Sciences Center New Orleans, LA 70112 (2)
Principal Investigator Centre for Targeted Protein Degradation (2)
Principal Investigator • Centre for Targeted Protein Degradation (2)
Proteomics Core Facility, University of Gothenburg, Sweden (2)
Aging $ Metabolism, Gene Translation Laboratory, Institute for Research in Biomedicine, The Barcelona Institute of Science and Technology, Barcelona, Catalonia, Spain. (1)
Assistant Professor, Department of Chemistry, The ScrippsResearch Institute (1)
Assistant Professor, RTGR (Tomato building), Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Gachibowli, Hyderabad, 500046. (1)
Assistant professor, Biochemistry and Molecular Biology, Johns Hopkin Bloomberg Schools of Public Health, USA. (1)
Associate Professor of Chemistry, University of California, Riverside (1)
Basic Immune System of Plants / Protein Mass Spectrometry Max Planck Institute for Plant Breeding Research Carl-von-Linne-Weg 10, 50829 Cologne, Germany (1)
Bavarian Center for Biomolecular Mass Spectrometry (BayBioMS) School of Life Sciences Technical University Munich (TUM) Gregor-Mendel-Straße 4 85354 Freising GERMANY Bavarian Center for Biomolecular Mass Spectrometry Technische Universität München Gregor-Mendel-Straße 4 85354 Freising DEUTSCHLAND / GERMANY (1)

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Efficient micro-scale basic reverse phase peptide fractionation for global and targeted proteomics

This work describes a micro-scale basic reverse phase liquid chromatographic (bRPLC) fractionation method that offers high reproducibility and efficiency for peptide mixtures from small (5-20 g) samples. Shotgun and targeted proteomic analysis were performed to detect differentially expressed prote...

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

2016-02-09 | MSV000079500 | MassIVE

Efficient Microscale Basic Reverse Phase Peptide Fractionation for Global and Targeted Proteomics.

Not available

S-EPMC5785084 | biostudies-literature

Reverse-phase liquid chromatography (RPLC) in Combination with Tip-Based Strong Cation Exchange (SCX-Tip) Fractionation, ReST, an Alternative Approach for Cross-linked Peptides analysis

Chemical cross-linking in combination with mass spectrometry (XL-MS) has emerged as a useful method for structural elucidation of proteins and protein complexes. Efficient enrichment procedures are necessary to analyze cross-linked products due to their relatively low abundance. Currently, strong ca...

ORGANISM(S): Homo sapiens (Human)

2022-06-16 | PXD023817 | Pride

Naive and DSS Murine Colon TMTpro bRP fractionation N-terminomics

The mammalian lysosomal protease legumain is often dysregulated in pathophysiological conditions including inflammation, neurodegeneration, and cancer, yet its proteolytic targets are poorly defined. To profile protease substrates degradomics techniques typically employ enrichment strategies to sele...

ORGANISM(S): Mus musculus (Mouse)

2025-09-29 | PXD060852 | Pride

Mouse Protease Fractionation Legumain N-terminomics Colon Colitis

Protein abundance estimation in HeLa cells

To estimate the amount of proteins in HeLa, whole cell digests were subjected to fractionation by off line reverse phase chromatography at basic pH followed by LC-MS/MS analysis

ORGANISM(S): Homo sapiens (Human)

2016-12-07 | PXD001441 | Pride

Hela proteome Lc-ms/ms

Yield and immunomodulatory effects of pneumococcal membrane vesicles change with bacterial growth phase

We investigated whether isolation of MVs during either the stationary-phase (24 h) or death-phase (48 h) affected their yield, immunogenicity and cytotoxicity in primary human immune cells. Death-phase vesicles showed higher yield than stationary-phase vesicles. Both vesicle types displayed excellen...

ORGANISM(S): Streptococcus pneumoniae (strain ATCC BAA-255 / R6)

2022-02-16 | PXD026462 | Pride

Proteomics Primary macrophages Death-phase Stationary-phase Bacterial membrane vesicles Vaccine Immunogenicity

Expression profiling data from mid-log phase cultures of wild type Vibrio campbellii BAA-1116

Small RNA and regulatory RNA discovery has been based on (i) computational predictions mainly in intergenic regions, (ii) microarray experiments, (iii) shotgun cloning of cDNA libraries, (iv) classical cloning of abundant small RNAs after size fractionation in polyacrylamide gels and (v) copurificat...

ORGANISM(S): Vibrio harveyi ATCC BAA-1116

2010-06-01 | E-GEOD-20960 | biostudies-arrayexpress

Arabidopsis Proteogenomics for identification of Ribo-seq predicted ORFs

Proteins were extracted from arabidopsis shoot or root tissue using Trypsin/LysC. 2D fractionation was performed with the first dimension being either SCX or basic reverse phase. Each fraction was then introduced to the MS using low pH reverse-phase chromatography. Resulting spectra were searched us...

ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702)

2020-03-02 | MSV000085044 | MassIVE

Employing complementary fractionation-based N-terminomics approaches enhances the identification of legumain cleavage events in naive and inflamed colon tissue.