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ProteomicsHadBD dehydratase from Mycobacterium tuberculosis FAS-II: a singular structure for a unique function
Mycolic acids (MAs) are a unique class of lipids that are essential for viability, virulence and persistence of Mycobacterium tuberculosis (Mtb). Therefore, enzymes involved in MAs biosynthesis represent important class of drug targets. We previously showed that (3R)-hydroxyacyl-ACP dehydratase (HAD...
ORGANISM(S): Mycobacterium bovis BCG str. Pasteur 1173P2 
2024-03-21 | PXD043977 | Pride
(3r)-hydroxyacyl-acp dehydratase Structure-function relationships. Mycolic acids Hydratase 2 Mycobacterium tuberculosis Hotdog fold Crystal structure Catalytic subunit Substrate specificity Fatty acid synthase type ii system 
ProteomicsProteasome composition analysis
Proteasome complexes diversity was studied by AP-MS approaches.
ORGANISM(S): Homo sapiens (Human) 
2016-07-08 | PXD001043 | Pride
Human Proteasome 
ProteomicsProcessing of microbial glycolipids in lysosome expands the repertoire of TLR2 agonists
The goal is to demonstrate that a fraction of lysosomal proteins purified from THP1 cells contains lipolytic activities capable of digesting a cell envelope compound (a tetra-acylated PIM) to generate acyl-forms not produced by Mtb.
ORGANISM(S): Homo sapiens (Human) 
2026-09-09 | PXD051087 | Pride
Receptor Cd1b Mycobacterium Acylation Lysosome Lipase 
ProteomicsAdipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamics
Extracellular vesicles are emerging key actors in adipocyte communication. Notably, small extracellular vesicles shed by adipocytes promote melanoma aggressiveness through fatty acid oxidation, with a heightened effect in obesity. However, the vesicular actors and cellular processes involved remain ...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2020-01-09 | PXD014487 | Pride
Exosome / fatty acid oxidation / lipophagy / melanoma / obesity / lc-msms 
ProteomicsProteome of primary, CRISPR/CAS9 modified mouse CD4+ T cells
We analyzed the total proteome of CD4+ T cells isolated from WT mice, and cultured to perform a CRISPR/CAS9 edition of their genome, in order to introduce an OST sequence at the C-terminus of proteins of interest (LAT or UBASH3A, n=6 biological replicates in each case). Control CD4+ T cells , isolat...
ORGANISM(S): Mus musculus (Mouse) 
2020-09-29 | PXD018766 | Pride
Primary cd4+ t cell Crispr/cas9 Whole cell lysate Proteome 
ProteomicsAnalysis of the interactome of CD6 in CRISPR/CAS9 modified mouse CD4+ T cells
Using a CRISPR/Cas9-based approach we engineer primary CD4+ T cells in which the CD6 protein was tagged with an affinity Twin-Strep-tag (OST) with the purpose of determining by quantitative mass spectrometry the composition and dynamics of the signalosome assembling around the tagged protein prior t...
ORGANISM(S): Mus musculus (Mouse) 
2020-09-29 | PXD018527 | Pride
Ap-ms Tcr Crispr/cas9 Primary t cell Interactome Ost tag 
ProteomicsAnalysis of the interactome of LAT in CRISPR/CAS9 modified mouse CD+ T cells
Using a CRISPR/Cas9-based approach we engineer primary CD4+ T cells in which the LAT protein was tagged with an affinity Twin-Strep-tag (OST) with the purpose of determining by quantitative mass spectrometry the composition and dynamics of the signalosome assembling around LAT prior to and followin...
ORGANISM(S): Mus musculus (Mouse) 
2020-09-29 | PXD018526 | Pride
Ap-ms Tcr Crispr/cas9 Interactome Primary cd+ t cell Ost tag 
ProteomicsAnalysis of CD5 interactome in primary mouse CD4+ T cells
CD4+ T cells were isolated from gene-targeted mouse expressing a One-Strep-tag (OST) at the C terminus of the CD5 protein, briefly expanded in vitro and we analyzed the interactome of their OST-tagged CD5 protein. Affinity purification of the OST tagged protein was performed using Streptactin beads,...
ORGANISM(S): Mus musculus (Mouse) 
2020-09-29 | PXD018552 | Pride
Ap-ms Tcr Primary cd4+ t cell Interactome Signalosome 
ProteomicsHuman Cytomegalovirus modifies placental small extracellular vesicle secretion and composition to enhance infection of fetal recipient cells
Human cytomegalovirus (hCMV) primo-infection, reinfection and/or reactivation is a major issue during pregnancy and affects 1% of live births in western countries, making hCMV the most frequently transmitted virus in utero. Despite the extensive research conducted so far, the pathophysiology of this...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Homo sapiens (Human) 
2022-10-13 | PXD029146 | Pride
Neural stem cells Hcmv Congenital infection Extracellular vesicles Placenta Cytotrophoblast 
ProteomicsFranco-Swiss HPP project (Chromosomes 2 and 14)
The dataset is a concatenation of nanoLC-MS/MS analyses performed on different sample types/sample preparations of human proteins on various instrumental platforms in the laboratory. A franco-swiss collaboration to identify missing human proteins on chromosomes 2 and 14 in the frame of the Human Pro...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-10-21 | MSV000080273 | MassIVE
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