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ProteomicsFerritin-mediated iron detoxification mediates hypothermia survival in Caenorhabditis elegans and murine neurons.
How animals rewire cellular programs to survive cold is a fascinating problem with potential biomedical implications, ranging from emergency medicine to space travel. Studying a hibernation-like response in the free-living nematode Caenorhabditis elegans, we uncovered a regulatory axis that enhances...
ORGANISM(S): Caenorhabditis elegans 
2022-10-14 | PXD034794 | Pride
Ferritin heavy chain Ferrous iron Foxo Ets-4 Neuroprotection Ferroxidase Daf-16 Fth1 Cold Ftn-1 Pqm-1 Ros Antioxidant Hibernation Iron Fe2+ Hypothermia 
TranscriptomicsDefining soma-specific/enriched and germline-specific/enriched transcripts
To define what genes are predominantly or specifically expressed in either soma or germline in C. elegans adults, total RNA was extracted from germline-less glp-4 mutant animals or from dissected gonads, respectively. Total RNA sequencing was peformed in duplicates. Four samples in total.
ORGANISM(S): Caenorhabditis elegans 
2015-01-08 | E-GEOD-62857 | biostudies-arrayexpress
TranscriptomicsFunctional characterization of C. elegans Y-box binding proteins reveals tissue-specific functions and a critical role in the formation of polysomes (RNA-Seq)
We present a basic characterization of the function of Y-box binding proteins in C. elegans. Besides playing an important role for fertility in the germline (all four CEY proteins), we found that the presence of CEY-1 and CEY-4 is essential for the assembly of larger polysomes in the soma. We theref...
ORGANISM(S): Caenorhabditis elegans 
2015-01-08 | E-GEOD-62858 | biostudies-arrayexpress
TranscriptomicsFunctional characterization of C. elegans Y-box binding proteins reveals tissue-specific functions and a critical role in the formation of polysomes (ribosome)
We present a basic characterization of the function of Y-box binding proteins in C. elegans. Besides playing an important role for fertility in the germline (all four CEY proteins), we found that the presence of CEY-1 and CEY-4 is essential for the assembly of larger polysomes in the soma. We theref...
ORGANISM(S): Caenorhabditis elegans 
2015-01-08 | E-GEOD-62859 | biostudies-arrayexpress
TranscriptomicsGLD-1 mRNA regulation
This SuperSeries is composed of the following subset Series: GSE33081: Identification of GLD-1 target mRNAs GSE33082: mRNA expression analysis in wild-type, gld-1 and cgh-1 mutant animals GSE33083: Polysome profiling of wild-type and gld-1 mutant animals GSE36713: mRNA expression analysis in mock an...
ORGANISM(S): Caenorhabditis elegans 
2012-06-17 | E-GEOD-33084 | biostudies-arrayexpress
TranscriptomicsIdentification of GLD-1 target mRNAs
To determine which mRNAs are bound by GLD-1 we performed GLD-1 and MYC (as a negative control) immunoprecipitation experiments on wildtype animals. A total of 6 samples was analyzed (triplicates of GLD-1 IPs and MYC IPs respectively)
ORGANISM(S): Caenorhabditis elegans 
2012-06-17 | E-GEOD-33081 | biostudies-arrayexpress
TranscriptomicsmRNA expression analysis in wild-type, gld-1 and cgh-1 mutant animals
To determine mRNA expression levels in wild-type, gld-1 (q485) mutant and cgh-1(tn691) mutant animals, RNA was purified from dissected gonads of young adult worms, using the PicoPur Kit. A total of 9 samples was analyzed (triplicates of wild-type, gld-1 mutant and cgh-1 mutant animals respectively)
ORGANISM(S): Caenorhabditis elegans 
2012-06-17 | E-GEOD-33082 | biostudies-arrayexpress
TranscriptomicsmRNA expression analysis in mock and cgh-1(RNAi) animals
To determine mRNA expression levels in mock and cgh-1(RNAi) animals, RNA was purified from young adult worms, using Trizol. A total of 6 samples was analyzed (triplicates of mock and cgh-1(RNAi) animals respectively)
ORGANISM(S): Caenorhabditis elegans 
2012-06-17 | E-GEOD-36713 | biostudies-arrayexpress
TranscriptomicsPolysome profiling of wild-type and gld-1 mutant animals
To determine the translational status of mRNAs in wild-type and gld-1 mutant animals, we analyzed total and polysomal RNA levels in both strains by tiling arrays A total of 16 samples was analyzed (quadruplicates of wild-type_total, wild-type_polysomal, gld-1_total, gld-1_polysomal RNA respectively)
ORGANISM(S): Caenorhabditis elegans 
2012-06-17 | E-GEOD-33083 | biostudies-arrayexpress
TranscriptomicsFunctional characterization of C. elegans Y-box binding proteins reveals tissue-specific functions and a critical role in the formation of polysomes (RIP-Seq)
We present a basic characterization of the function of Y-box binding proteins (YBPs) in C. elegans. YBPs in other organisms are known RNA-binding proteins. Our global analysis of associated mRNAs supports a role of YBPs as general mRNA binders in C.elegans. FLAG IPs were performed on transgenic anim...
ORGANISM(S): Caenorhabditis elegans 
2015-01-08 | E-GEOD-62856 | biostudies-arrayexpress
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